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4.1.3.1: isocitrate lyase

This is an abbreviated version!
For detailed information about isocitrate lyase, go to the full flat file.

Word Map on EC 4.1.3.1

Reaction

isocitrate
=
succinate
 +
glyoxylate

Synonyms

AceA, acuD, citrate lyase, D-threo-isocitrate: glyoxylate lyase, EgGCE, FPICL1, ICL, ICL1, ICL2, isocitrase, isocitratase, isocitrate lyase, isocitrate lyase 1, isocitrate lyase 2, isocitric lyase, isocitritase, lyase, isocitrate, MtbIcl, PDP, petal death protein, SSO1333, threo-DS-Isocitrate glyoxylate-lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.1 isocitrate lyase

Temperature Stability

Temperature Stability on EC 4.1.3.1 - isocitrate lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
no loss of activity after 30 min
35
-
no enzyme activity
40 - 50
-
stable for 1 h
45
purified enzyme, half-life of the wild-type enzyme is 40 min, half-lives of mutants E219A, Q211H, and Q221K are 25 min, 12 min, and 15 min
51
-
native enzyme, apparent melting temperature (Tm), far-UV CD (222 nm), major part of enzyme resistant to thermal unfolding despite almost complete loss of activity above 50°C, catalytic domain unfolded
55.5
-
50% activity lost after 43 min, in presence of 0.4 M KCl 50% activity lost after 129 min
56
-
1 mM MgCl2, first Tm of biphasic thermal denaturation curve (far-UV CD (222 nm)), loss of secondary structure similar to native enzyme, catalytic domain unfolded
65
-
40% activity lost after 15 min
66
-
2.5 mM MgCl2, second Tm of biphasic thermal denaturation curve (far-UV CD (222 nm)), almost complete loss of secondary structure, noncatalytic alpha/beta barrel unfolded
90
-
1 mM MgCl2, second Tm of biphasic thermal denaturation curve (far-UV CD (222 nm)), almost complete loss of secondary structure, noncatalytic alpha/beta barrel unfolded
additional information