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Literature summary for 4.1.3.1 extracted from

  • Tahama, H.; Shinoyama, H.; Ando, A.; Fujh, T.
    Purification and characterization of isocitrate lyase from Rhodopseudomonas sp. No. 7 (1990), Agric. Biol. Chem., 54, 3177-3183.
No PubMed abstract available
Search for more literature for 4.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 30% inhibition at 0.1 mM Rhodopseudomonas sp.
iodoacetate 70% inhibition at 10 mM Rhodopseudomonas sp.
malate
-
 Rhodopseudomonas sp.
N-ethylmaleimide 40% inhibition at 1 mM Rhodopseudomonas sp.
p-chloromercuribenzoate 70% inhibition at 0.1 mM Rhodopseudomonas sp.
succinate
-
 Rhodopseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.136
-
 DL-isocitrate
-
 Rhodopseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ Ba2+ less effective activator than Mg2+ Rhodopseudomonas sp.
Mg2+ non-essential activator Rhodopseudomonas sp.
Mn2+ Mn2+ less effective activator than Mg2+ Rhodopseudomonas sp.
Ni2+ less efficient activator than Mg2+ Rhodopseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 4 * 62000, SDS-PAGE Rhodopseudomonas sp.
250000
-
 gel filtration Rhodopseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate Rhodopseudomonas sp. glyoxylate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodopseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.73
-
-
 Rhodopseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
 Rhodopseudomonas sp. succinate + glyoxylate
-
 ?
isocitrate glyoxylate cycle Rhodopseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 62000, SDS-PAGE Rhodopseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Rhodopseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 low stability at temperatures above Rhodopseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Rhodopseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 stable Rhodopseudomonas sp.