Cloned (Comment) | Organism |
---|---|
gene icl, expression of N-terminally His6-tagged enzyme in Escherichia coli strains XL-1 Blue, KM22 (a DELTArecBCD mutant), and ACA421, the latter is defective in isocitrate lyase | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | Azotobacter vinelandii | - |
succinate + glyoxylate | - |
? | |
isocitrate | Azotobacter vinelandii IAM1078 | - |
succinate + glyoxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | A8C781 | gene icl | - |
Azotobacter vinelandii IAM1078 | A8C781 | gene icl | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Azotobacter vinelandii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | cell growth is similar in glucose- or sodium acetate-medium, but the enzyme activity is 43fold higher in acetate-grown cells | Azotobacter vinelandii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate | - |
Azotobacter vinelandii | succinate + glyoxylate | - |
? | |
isocitrate | - |
Azotobacter vinelandii IAM1078 | succinate + glyoxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ICL | - |
Azotobacter vinelandii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azotobacter vinelandii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the amino acid residue at position 504 is involved in thermostability of the ICL enzymes | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Azotobacter vinelandii |
Organism | Comment | Expression |
---|---|---|
Azotobacter vinelandii | enzyme expression is induced by acetate | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the isocitrate lyase ICL subfamily 3, one of two eubacterial groups, it has a substitution of M504I compared to the cold-adapted ICLs, rendering is less thermostable, phylogenetic analysis, overview | Azotobacter vinelandii |