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Literature summary for 4.1.3.1 extracted from
- Enzyme catalysis in microgravity: steady-state kinetic analysis of the isocitrate lyase reaction (2003), Biophys. Chem., 103, 169-177.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.039 | - |
isocitrate | pH 7, standard g, cleavage reaction | Pinus pinea |  |
| 0.043 | - |
isocitrate | pH 7, microgravity, cleavage reaction | Pinus pinea | |
| 0.053 | - |
succinate | pH 7, microgravity, condensation reaction | Pinus pinea | |
| 0.063 | - |
succinate | pH 7, standard g, condensation reaction | Pinus pinea | |
| 0.088 | - |
glyoxylate | pH 7, microgravity, condensation reaction | Pinus pinea | |
| 0.092 | - |
glyoxylate | pH 7, standard g, condensation reaction | Pinus pinea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | Pinus pinea | - |
succinate + glyoxylate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pinus pinea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pinus pinea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| isocitrate = succinate + glyoxylate | kinetic mechanism is the same in microgravity and in standard g controls | Pinus pinea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinating | Pinus pinea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate | - |
Pinus pinea | succinate + glyoxylate | - |
r | |
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