3.1.1.6: acetylesterase
This is an abbreviated version!
For detailed information about acetylesterase, go to the full flat file.
Word Map on EC 3.1.1.6
-
3.1.1.6
-
esterases
-
xylans
-
deacetylation
-
saccharification
-
carbohydrate-active
-
glucuronoyl
-
isavs
-
receptor-destroying
-
acetylxylan
-
cazymes
-
glucuronoxylans
-
alpha-naphthyl
-
arabinoxylans
-
methylesterase
-
lignin-carbohydrate
-
arylesterase
-
feruloylated
-
hemicellulolytic
-
4-methylumbelliferyl
-
hemagglutinin-esterase
-
xylooligosaccharides
-
beta-naphthyl
-
9-o-acetylated
-
xylopyranosyl
-
orthomyxoviridae
-
molecular biology
-
synthesis
-
industry
-
paper production
-
analysis
- 3.1.1.6
- esterases
- xylans
-
deacetylation
-
saccharification
-
carbohydrate-active
-
glucuronoyl
- isavs
-
receptor-destroying
- acetylxylan
-
cazymes
- glucuronoxylans
-
alpha-naphthyl
- arabinoxylans
-
methylesterase
-
lignin-carbohydrate
- arylesterase
-
feruloylated
-
hemicellulolytic
-
4-methylumbelliferyl
- hemagglutinin-esterase
- xylooligosaccharides
-
beta-naphthyl
-
9-o-acetylated
-
xylopyranosyl
- orthomyxoviridae
- molecular biology
- synthesis
- industry
- paper production
- analysis
Reaction
Synonyms
6-O-deacetylase, ACE, AcE1, acetate ester-hydrolysing esterase, acetic acid esterase, Acetic ester hydrolase, Acetyl esterase, acetyl xylan esterase, acetyl-esterase, acetylesterase CE16, AE, AE206, Aes, Aes1, alpha/beta-acetylesterase, Axe3, BS2, C-esterase, C20orf3, carbohydrate acetylesterase, carbohydrate esterase, CE16, CE16 acetyl esterase, CE16A, CE2, CE2A, CE2B, CE2C, Chloroesterase, Citrus acetylesterase, EST-4, Est1, EST2, Est24, ESTK, GAE, geranyl acetate cleaving esterase, haemagglutinin-esterase, HE, HerE, More, non-specific acetyl esterase, p-nitrophenyl acetate esterase, PAE1, PAE11, PAE2, PAE3, PAE4, PAE5, PAE6, PAE7, PAE8, PAE9, pectin acetylesterase, ragi acetic acid esterase, TM0077, ybaC
ECTree
3.1.1.6 acetylesteraseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.1.1.6 - acetylesterase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
(R)-1,2-O-isopropylidene glycol acetate + H2O
(R)-1,2-O-isopropylidene glycol + acetate
-
-
-
-
?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O
(R)-1,2-O-isopropylidene glycol + butyrate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)
-
?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O
(R)-1,2-O-isopropylidene glycol + caproate
-
fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells
-
?
1-naphthyl butyrate + H2O
1-naphthol + butyrate
36% activity compared to 1-naphthyl acetate
-
-
?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
-
determination of enantiopreference, wild-type R enantioselectivity is 42
spectrophotometric assay coupling released acetate to NADH generation
-
?
2-ethylcyclohex-1-en-1-yl acetate + H2O
2-ethylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-isopropylcyclohex-1-en-1-yl acetate + H2O
2-isopropylcyclohexanone + acetate
-
isoenzyme Est I, 10% conversion of substrate with R-configuration of product
-
-
?
2-methylcyclohex-1-en-1-yl acetate + H2O
(S)-2-methylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-n-propylcyclohex-1-en-1-yl acetate + H2O
2-n-propylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
4-methylumbelliferyl acetate + H2O
4-methylumbelliferone + acetate
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + vinyl acetate
4-nitrophenyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 70.2%
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenyl D-xylopyranose
4 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate
beta-xylosidase-coupled assay
-
?
4-nitrophenyl formate + H2O
4-nitrophenol + formate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
preferred substrate
-
-
?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
-
-
-
?
7-aminocephalosporanic acid + H2O
deacetylated 7-aminocephalosporanic acid + acetate
-
-
-
-
?
acetyl xylan + H2O
xylan + acetate
-
from beech wood or oat spelt
-
?
acetylated hemicellulose + H2O
? + acetate
-
the best substrate of the enzyme is 3-O-acetylated Xylp residue followed by 4-O-acetylated Xylp residue with a free vicinal hydroxyl group
-
-
?
acetylglucuronoxylan + H2O
glucuronoxylan + acetate
-
the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation
-
-
?
acetylxylan + H2O
xylan + acetate
-
60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan
-
-
?
bovine submaxillary mucin + H2O
deacetylated bovine submaxillary mucin + acetate
-
-
-
?
geraniol acetate + H2O
geraniol + acetate
-
enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development
-
?
isoamyl acetate + H2O
isoamyl alcohol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
kojic acid + vinyl acetate
7-O-acetyl-kojic acid + vinyl alcohol
-
yield is 30.9%
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O
methyl beta-D-xylopyranoside + 3 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O
methyl 2-O-acetyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O
methyl beta-D-xylopyranoside + 2 acetate
-
all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h
-
-
?
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O
methyl xylotriose + acetate
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
methyl 3-O-acetyl-beta-D-glucopyranoside + vinyl alcohol
-
yield is 56.4%
-
-
?
methyl-beta-D-xylopyranoside + vinylacetate
methyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside)
analysis by thin-layer chromatography
-
?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
penta-O-acetyl-alpha-D-glucose + H2O
?
-
complete and regioselective removal of primary acetyl-group in 6-position
-
-
?
racemic 1-phenylethyl acetate + H2O
(1R)-phenylethanol + (1S)-phenylethanol + acetate
-
-
-
-
?
triacetin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O
[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
-
fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol
high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O
?
-
highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
(acetyloxy)(phenyl)acetic acid + H2O
(S)-mandelic acid + (2R)-(acetyloxy)(phenyl)ethanoic acid
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
-
-
-
?
1-naphthyl acetate + H2O
1-naphthol + acetate
highest activity (100%)
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
-
isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product
-
-
?
2-benzylcyclohex-1-en-1-yl acetate + H2O
2-benzylcyclohexanone + acetate
-
isoenzyme Est II, 11% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
-
isoenzyme Est I, 20% conversion of substrate with R-configuration of product
-
-
?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O
2-n-pentylcyclohexanone + acetate
-
isoenzyme Est II, 16% conversion of substrate with S-configuration of product
-
-
?
2-naphthyl acetate + H2O
2-naphthol + acetate
about 40% activity compared to 1-naphthyl acetate
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
-
isoenzyme Est I, 21% conversion of substrate with S-configuration of product
-
-
?
2-t-butylcyclohex-1-en-1-yl acetate + H2O
2-t-butylcyclohexanone + acetate
-
isoenzyme Est II, 20% conversion of substrate with R-configuration of product
-
-
?
3,6-diacetylmorphine + H2O
morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3,6-diacetylmorphine + H2O
morphine + acetate
-
i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
3-O-methyl-D-glucopyranoside + vinyl acetate
6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O
4-nitrophenyl-beta-D-xylopyranoside + acetate
-
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
serine esterase activity
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
determination of specific activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
product determination by NMR and mass spectroscopy
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
-
highest activity
-
-
?
4-nitrophenyl acetate + H2O
4-nitrophenol + acetate
5 min, 30°C, pH 5.8, for kinetic analyses: 0.05-5 mM substrate in 100 mM sodium phosphate buffer
analysis by spectrophotometry at 410 nm
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
-
lowest activity
-
-
?
4-nitrophenyl butyrate + H2O
4-nitrophenol + butyrate
5 min, 30°C, pH 5.8
-
-
?
4-nitrophenyl propionate + H2O
4-nitrophenol + propionate
-
-
-
-
?
6-acetylmorphine + H2O
morphine + acetate
-
substrate specificity
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
EST1, non-denaturating PAGE, pH7.4
zymogram analyses using Fast Blue PR salt
-
?
alpha-naphthyl acetate + H2O
alpha-naphthol + acetate
-
15 min, 50°C, pH 6.5
-
-
?
astragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
astragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
beta-naphthyl acetate + H2O
beta-naphthol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8
SDS-PAGE and in gel-zymography
-
?
cellulose acetate + H2O
cellulose + acetate
-
also in form of acetylated starch, water-soluble and water-insoluble substrate, specific for acetyl substituents at the C2- and C3-positions, C6-acetyl is not hydrolyzed
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
-
isozyme CE2B shows 89% transacetylation conversion
-
-
?
D-galactose + vinyl acetate
? + vinyl alcohol
-
isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 86% transacetylation conversion
-
-
?
D-glucose + vinyl acetate
6-O-acetyl-D-glucopyranose + vinyl alcohol
-
isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion
-
-
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 71% transacetylation conversion
-
-
?
D-mannose + vinyl acetate
6-O-acetyl-D-mannopyranose + vinyl alcohol
-
isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
ethyl acetate + H2O
ethanol + acetate
-
25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts
-
-
?
isoastragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside I + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
isoastragaloside II + H2O
astragaloside IV + ?
-
-
i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl alpha-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
methyl beta-D-glucopyranoside + vinyl acetate
? + vinyl alcohol
-
-
-
-
?
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
-
-
-
ir
O-acetyl-1,4-beta-xylooligosaccharide + H2O
1,4-beta-xylooligosaccharide + acetic acid
-
-
-
-
ir
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
-
?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O
4-O-methyl-D-glucurono-D-xylan + acetic acid
-
-
-
ir
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
EST1, pH 7.4, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
spectrophotometry, 400 nm
-
?
p-nitrophenyl acetate + H2O
p-nitrophenol + acetate
-
10 min, 50°C, pH 6.5
-
-
?
p-nitrophenyl hexanoate + H2O
p-nitrophenol + hexanoate
-
-
-
-
?
p-nitrophenyl propionate + H2O
p-nitrophenol + propionate
-
-
-
-
?
phenyl acetate + H2O
phenol + acetate
-
sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA
spectrophotometry, absorbance at 270 nm
-
?
tributyrin + H2O
?
-
EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum
titration assay
-
?
xanthan + H2O
?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
xanthan + H2O
?
the enzyme removes 57% of all acetyl groups present in xanthan after a 72 h incubation which corresponds to the removal of about 70% of all acetyl groups positioned on the inner mannose unit
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
enzyme has receptor-destroying enzyme function
-
?
additional information
?
-
-
substrate specificity and regioselectivity, deacetylation activity of the enzyme is not restricted to polymers with beta-1,4 glycosidic bonds, no activity with cellulose sulfate, the enzyme enhances endoglucanase, but is itself not influenced by endoglucanase
-
?
additional information
?
-
-
the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters
-
-
?
additional information
?
-
-
1-(4-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-chlorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-methylphenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3,4-difluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate are substrates of double mutant E188W/M193C, conversion with inversed enantioselectivity
-
-
?
additional information
?
-
-
1-phenyl-1-(trifluoromethyl)but-2-yn-1-yl acetate and 1-phenyl-1-(trifluoromethyl)prop-2-en-1-yl acetate are no substrates of neither wild-type nor any mutant
-
-
?
additional information
?
-
-
size of residue at position 188 (from Asp to Trp) determines enantioselectivity and -preference
-
-
?
additional information
?
-
-
no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside
-
-
?
additional information
?
-
-
the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors
-
-
?
additional information
?
-
-
removal of acetyl group in 3-position of beta-lactams with 98% conversion and 91-93% product yield
-
-
?
additional information
?
-
-
the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties
-
-
?
additional information
?
-
-
activated Sepharose CL6B-bound activity, pH 8
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
activity of EST1 only on short chain triglyceride substrates
-
-
?
additional information
?
-
-
EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1
-
-
?
additional information
?
-
-
no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate
-
-
?
additional information
?
-
-
no activity of EST1 on triacylglyceride esters tricaprylin and triolein
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2
-
-
?
additional information
?
-
-
the enzyme has no activity toward 4-nitrophenyl valerate, 4-nitrophenyl caproate, 4-nitrophenyl palmitate, 4-nitrophenyl benzoate, 4-nitrophenyl phosphate, methyl benzoate, methyl 4-hydroxybenzoate, or methyl cyclohexane-1-carboxylate
-
-
?
additional information
?
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil
-
-
?
additional information
?
-
-
no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity
-
-
?
additional information
?
-
-
no activity with 4-nitrophenyl octanoate. The enzyme does not exhibit de-esterification activity toward an acetylated xylooligosaccharide extracted from hot-water treated eucalyptus
-
-
?
additional information
?
-
-
the enzyme has no activity with acetylated xylans
-
-
?
additional information
?
-
-
no activity is detected on xylan or acetylated xylan or N-acetyl-D-glucosamine
-
-
?
additional information
?
-
new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1
-
-
?
additional information
?
-
-
acetylesterases generally prefer aliphatic substrates involving acetic acid
-
-
?
additional information
?
-
-
isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities
-
-
?
