Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sinorhizobium meliloti |
Crystallization (Comment) | Organism |
---|---|
using 0.2 M ammonium phosphate (pH 4.6), and 20% (w/v) polyethylene glycol 3350 | Sinorhizobium meliloti |
Protein Variants | Comment | Organism |
---|---|---|
F152A | the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis | Sinorhizobium meliloti |
M155A | the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis | Sinorhizobium meliloti |
N96A | the mutant has almost no activity | Sinorhizobium meliloti |
R69A | the mutant has about 25% activity for 1-naphthyl acetate hydrolysis compared to the wild type enzyme | Sinorhizobium meliloti |
S13A | the mutant shows complete loss of activity | Sinorhizobium meliloti |
W151A | the mutant has activities similar to that of wild type enzyme for 1-naphthyl acetate hydrolysis | Sinorhizobium meliloti |
Y101A | the mutant has almost no activity | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | Q92XZ6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | highest activity (100%) | Sinorhizobium meliloti | 1-naphthol + acetate | - |
? | |
1-naphthyl butyrate + H2O | 36% activity compared to 1-naphthyl acetate | Sinorhizobium meliloti | 1-naphthol + butyrate | - |
? | |
2-naphthyl acetate + H2O | about 40% activity compared to 1-naphthyl acetate | Sinorhizobium meliloti | 2-naphthol + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Sinorhizobium meliloti | 4-methylumbelliferone + acetate | - |
? | |
acetyl alginate + H2O | - |
Sinorhizobium meliloti | alginate + acetate | - |
? | |
cellulose acetate + H2O | - |
Sinorhizobium meliloti | cellulose + acetate | - |
? | |
D-glucose penta-acetate + H2O | - |
Sinorhizobium meliloti | ? | - |
? | |
additional information | no activity with 1-naphthyl phosphate, 4-methylumbelliferyl phosphate, linalyl acetate, alpha-terpinyl acetate, glyceryl trioleate, fish oil, or olive oil | Sinorhizobium meliloti | ? | - |
? | |
tert-butyl acetate + H2O | - |
Sinorhizobium meliloti | 2-methylpropane + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | - |
Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
carbohydrate acetylesterase | - |
Sinorhizobium meliloti |
Est24 | - |
Sinorhizobium meliloti |