Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.000109
4-nitrophenyl butyrate, purified recombinant enzyme
0.000213
4-nitrophenyl formate, purified recombinant enzyme
0.000225
-
mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000236
-
mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.000342
-
wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000347
-
wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.00507
-
with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35°C
0.021
Candida bororiensis
-
-
0.027
-
purified native enzyme, substrate ragi
0.061
-
purified native enzyme, substrate larch wood xylan
0.082
-
substrate p-nitrophenyl laurate, pH 7.0, 25°C
0.251
-
substrate p-nitrophenyl acetate, pH 7.0, 25°C
0.282
-
purified native enzyme, substrate gum karaya
0.297
-
substrate p-nitrophenyl decanoate, pH 7.0, 25°C
0.352
-
substrate p-nitrophenyl hexanoate, pH 7.0, 25°C
0.369
-
substrate p-nitrophenyl propionate, pH 7.0, 25°C
0.474
-
substrate p-nitrophenyl butyrate, pH 7.0, 25°C
0.52
-
crude enzyme, at pH 8.0 and 35°C
0.85
recombinant enzyme, after CM Sepharose FF column
1.12
recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate
1.21
4-nitrophenyl acetate, purified recombinant enzyme
10.3
-
with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
1027
-
alpha-naphthyl acetate, pH 6.5
104.85
-
with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35°C
13.3
-
with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
2.37
-
with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35°C
20.5
4-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30°C, pH 5.8
221.9
-
after 423.39fold purification, at pH 8.0 and 35°C
250
-
pH 7.1, 65°C, purified recombinant wild-type enzyme
270
-
pH 7.1, 65°C, purified recombinant mutant T74A
3.38
-
purified native enzyme, substrate alpha-naphthyl acetate
324
-
(R,S)-1,2-O-isopropylidene glycol acetate, EST1, after preparative electrophoresis
328
-
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
333
-
wild-type, using 4-nitrophenyl acetate as substrate
35
-
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
395
-
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
4
-
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
4.44
-
purified native enzyme, substrate wheat
46.5
-
mutant E188W/M193C, using 4-nitrophenyl acetate as substrate
5.39
-
purified native enzyme, substrate 4-nitrophenyl acetate
5.64
-
with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
60
-
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
71
-
p-nitrophenyl acetate, pH 6.5
8
-
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
9.63
-
p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5
0.21
4-nitrophenyl acetate, over-expression culture supernatant
0.21
recombinant enzyme, culture supernatant
183
-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
183
-
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
2
-
isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
2
-
isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
3
-
isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
3
-
isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
5
-
-
5
-
isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
additional information
-
substrate specificity
additional information
-
-
additional information
-
-
additional information
-
isoforms with pI-values 4.2 and 5.0 are the most active ones in all samples
additional information
-
(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
(S)-1,2-O-isopropylidene glycol acetate, EST1, 1.2 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
triacetin as substrate for EST1, 4.06 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
-
tributyrin as substrate for EST1, 2.8 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1
additional information
extremly low activities against 4-nitrophenyl formate, butyrate, or canoate
additional information
-
mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain
additional information
-
mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain