3.1.1.6: acetylesterase

This is an abbreviated version!
For detailed information about acetylesterase, go to the full flat file.

Word Map on EC 3.1.1.6

Reaction

An acetic ester
+
H2O
=
an alcohol
+
acetate

Synonyms

More, Acetic ester hydrolase, Acetyl esterase, C-esterase, Chloroesterase, Citrus acetylesterase, p-nitrophenyl acetate esterase, HE, ACE, CE2, EST2, Est1, haemagglutinin-esterase, AE, GAE, geranyl acetate cleaving esterase, HerE, acetyl xylan esterase, Aes, EST-4, alpha/beta-acetylesterase, acetyl-esterase, acetic acid esterase, ragi acetic acid esterase, non-specific acetyl esterase, acetate ester-hydrolysing esterase, carbohydrate esterase, Aes1, BS2, C20orf3, 6-O-deacetylase, CE2B, CE2C, CE2A, Axe3, ESTK, TM0077, pectin acetylesterase, PAE1, CE16, carbohydrate acetylesterase, AcE1, Est24, CE16 acetyl esterase, CE16A, AE206, ybaC, acetylesterase CE16, PAE2, PAE3, PAE4, PAE5, PAE6, PAE7, PAE8, PAE9, PAE11

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.6 acetylesterase

Specific Activity

Specific Activity on EC 3.1.1.6 - acetylesterase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000109
4-nitrophenyl butyrate, purified recombinant enzyme
0.000213
4-nitrophenyl formate, purified recombinant enzyme
0.000225
-
mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000236
-
mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.000342
-
wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity
0.000347
-
wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity
0.00507
-
with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35°C
0.021
Candida bororiensis
-
-
0.027
-
purified native enzyme, substrate ragi
0.061
-
purified native enzyme, substrate larch wood xylan
0.082
-
substrate p-nitrophenyl laurate, pH 7.0, 25°C
0.251
-
substrate p-nitrophenyl acetate, pH 7.0, 25°C
0.282
-
purified native enzyme, substrate gum karaya
0.297
-
substrate p-nitrophenyl decanoate, pH 7.0, 25°C
0.352
-
substrate p-nitrophenyl hexanoate, pH 7.0, 25°C
0.369
-
substrate p-nitrophenyl propionate, pH 7.0, 25°C
0.474
-
substrate p-nitrophenyl butyrate, pH 7.0, 25°C
0.52
-
crude enzyme, at pH 8.0 and 35°C
0.85
recombinant enzyme, after CM Sepharose FF column
1.12
recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate
1.21
4-nitrophenyl acetate, purified recombinant enzyme
10.3
-
with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
1027
-
alpha-naphthyl acetate, pH 6.5
104.85
-
with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35°C
1104
-
-
13.3
-
with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
16050
-
purified enzyme
2.37
-
with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35°C
20.5
4-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30°C, pH 5.8
221.9
-
after 423.39fold purification, at pH 8.0 and 35°C
250
-
pH 7.1, 65°C, purified recombinant wild-type enzyme
270
-
pH 7.1, 65°C, purified recombinant mutant T74A
3.38
-
purified native enzyme, substrate alpha-naphthyl acetate
324
-
(R,S)-1,2-O-isopropylidene glycol acetate, EST1, after preparative electrophoresis
328
-
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
333
-
wild-type, using 4-nitrophenyl acetate as substrate
35
-
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
376
-
-
395
-
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C
4
-
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
4.44
-
purified native enzyme, substrate wheat
46.5
-
mutant E188W/M193C, using 4-nitrophenyl acetate as substrate
5.39
-
purified native enzyme, substrate 4-nitrophenyl acetate
5.64
-
with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C
60
-
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C
71
-
p-nitrophenyl acetate, pH 6.5
71.4
-
purified enzyme
8
-
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C
9.63
-
p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5
additional information