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Literature summary for 3.1.1.6 extracted from

  • Levisson, M.; Han, G.W.; Deller, M.C.; Xu, Q.; Biely, P.; Hendriks, S.; Ten Eyck, L.F.; Flensburg, C.; Roversi, P.; Miller, M.D.; McMullan, D.; von Delft, F.; Kreusch, A.; Deacon, A.M.; van der Oost, J.; Lesley, S.A.; Elsliger, M.A.; Kengen, S.W.; Wilson, I.A.
    Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima (2012), Proteins, 80, 1545-1559.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain DL41 Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20% (w/v) PEG-3000, 0.1 M HEPES pH 7.5, 0.2 M NaCl (selenomethionine-substituted enzyme) or nanodrop vapor diffusion method, using 0.2 M calcium acetate hydrate, 20% (w/v) PEG 3350, pH 7.3 at 20°C (native enzyme) Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
paraoxon competitive irreversible inhibitor Thermotoga maritima
phenylmethylsulfonyl fluoride competitive irreversible inhibitor Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.137
-
 4-nitrophenyl propionate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
0.185
-
 4-nitrophenyl acetate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
3.6
-
 2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
4
-
 4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
4.2
-
 3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-chelating resin column chromatography and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Thermotoga maritima 1-naphthol + acetate
-
 ?
2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga maritima ?
-
 ?
3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga maritima ?
-
 ?
4-nitrophenyl acetate + H2O
-
 Thermotoga maritima 4-nitrophenol + acetate
-
 ?
4-nitrophenyl propionate + H2O
-
 Thermotoga maritima 4-nitrophenol + propionate
-
 ?
4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga maritima ?
-
 ?
7-aminocephalosporanic acid + H2O
-
 Thermotoga maritima ?
-
 ?
cephalosporin C + H2O
-
 Thermotoga maritima deacetylcephalosporin C + acetate
-
 ?
glucose penta-acetate + H2O high activity Thermotoga maritima ?
-
 ?
additional information no activity is detected on xylan or acetylated xylan or N-acetyl-D-glucosamine Thermotoga maritima ?
-
 ?

Synonyms

Synonyms Comment Organism
Acetyl esterase
-
 Thermotoga maritima
TM0077
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
100
-
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 the enzyme is not stable at 100°C, resulting in a half-life of less than 5 min Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
41.3
-
 4-nitrophenyl propionate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
57.5
-
 4-nitrophenyl acetate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
70.1
-
 3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
76.1
-
 2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
78.6
-
 4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.7
-
 3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
19.7
-
 4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
21.1
-
 2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
301.5
-
 4-nitrophenyl propionate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima
310.8
-
 4-nitrophenyl acetate in 50 mM citrate-phosphate (pH 6) at 70°C Thermotoga maritima