Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acetivibrio thermocellus |
expressed in Escherichia coli BL21(DE3) cells | Cellvibrio japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
1.55 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
2.46 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
4.31 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
4.42 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Cellvibrio japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography | Acetivibrio thermocellus |
immobilized metal affinity chromatography | Cellvibrio japonicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
2 | - |
isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
3 | - |
isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
3 | - |
isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
4 | - |
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
5 | - |
isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
8 | - |
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
35 | - |
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
60 | - |
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
183 | - |
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
183 | - |
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
328 | - |
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
395 | - |
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
3-O-methyl-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol | - |
? | |
3-O-methyl-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol | - |
? | |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
6-O-acetyl-D-mannopyranose + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
6-O-acetyl-D-mannopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
alpha,alpha-trehalose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
cellobiose + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
cellobiose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
D-galactose + vinyl acetate | isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion | Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
D-galactose + vinyl acetate | isozyme CE2B shows 89% transacetylation conversion | Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
D-glucose + vinyl acetate | isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion | Cellvibrio japonicus | 6-O-acetyl-D-glucopyranose + vinyl alcohol | - |
? | |
D-glucose + vinyl acetate | isozyme CE2B shows 86% transacetylation conversion | Acetivibrio thermocellus | 6-O-acetyl-D-glucopyranose + vinyl alcohol | - |
? | |
D-mannose + vinyl acetate | isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion | Cellvibrio japonicus | 6-O-acetyl-D-mannopyranose + vinyl alcohol | - |
? | |
D-mannose + vinyl acetate | isozyme CE2B shows 71% transacetylation conversion | Acetivibrio thermocellus | 6-O-acetyl-D-mannopyranose + vinyl alcohol | - |
? | |
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
methyl alpha-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
methyl alpha-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
methyl beta-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
methyl beta-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
additional information | no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside | Acetivibrio thermocellus | ? | - |
? | |
additional information | no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside | Cellvibrio japonicus | ? | - |
? | |
additional information | the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors | Acetivibrio thermocellus | ? | - |
? | |
additional information | the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors | Cellvibrio japonicus | ? | - |
? | |
xylobiose + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
xylobiose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-O-deacetylase | - |
Acetivibrio thermocellus |
6-O-deacetylase | - |
Cellvibrio japonicus |
ACE | - |
Acetivibrio thermocellus |
ACE | - |
Cellvibrio japonicus |
Acetyl esterase | - |
Acetivibrio thermocellus |
Acetyl esterase | - |
Cellvibrio japonicus |
CE2 | - |
Acetivibrio thermocellus |
CE2A | isozyme | Cellvibrio japonicus |
CE2B | isozyme | Cellvibrio japonicus |
CE2C | isozyme | Cellvibrio japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41.6 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
241.6 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
1331 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
2101 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
3210 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.42 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
56.08 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
541.5 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
1356 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
2599 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus |