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Literature summary for 3.1.1.6 extracted from
- Carbohydrate esterases of family 2 are 6-O-deacetylases (2010), FEBS Lett., 584, 543-548.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Acetivibrio thermocellus |
| expressed in Escherichia coli BL21(DE3) cells | Cellvibrio japonicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.24 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus |  |
| 1.55 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 2.46 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
| 4.31 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 4.42 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Cellvibrio japonicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal affinity chromatography | Acetivibrio thermocellus |
| immobilized metal affinity chromatography | Cellvibrio japonicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
| 2 | - |
isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 3 | - |
isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 3 | - |
isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 4 | - |
isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 5 | - |
isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 8 | - |
isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
| 35 | - |
isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
| 60 | - |
isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 183 | - |
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Acetivibrio thermocellus |
| 183 | - |
isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 328 | - |
isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
| 395 | - |
isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C | Cellvibrio japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| 2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| 3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| 3-O-methyl-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol | - |
? | |
| 3-O-methyl-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | 6-O-acetyl-3-O-methyl-D-glucopyranoside + vinyl alcohol | - |
? | |
| 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| 4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| 6-O-acetyl-D-mannopyranose + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| 6-O-acetyl-D-mannopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| alpha,alpha-trehalose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| cellobiose + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| cellobiose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| D-galactose + vinyl acetate | isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion | Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| D-galactose + vinyl acetate | isozyme CE2B shows 89% transacetylation conversion | Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| D-glucose + vinyl acetate | isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion | Cellvibrio japonicus | 6-O-acetyl-D-glucopyranose + vinyl alcohol | - |
? | |
| D-glucose + vinyl acetate | isozyme CE2B shows 86% transacetylation conversion | Acetivibrio thermocellus | 6-O-acetyl-D-glucopyranose + vinyl alcohol | - |
? | |
| D-mannose + vinyl acetate | isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion | Cellvibrio japonicus | 6-O-acetyl-D-mannopyranose + vinyl alcohol | - |
? | |
| D-mannose + vinyl acetate | isozyme CE2B shows 71% transacetylation conversion | Acetivibrio thermocellus | 6-O-acetyl-D-mannopyranose + vinyl alcohol | - |
? | |
| D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| methyl 6-O-acetyl-beta-D-glucopyranoside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
| methyl 6-O-acetyl-beta-D-glucopyranoside + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| methyl alpha-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| methyl alpha-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| methyl beta-D-glucopyranoside + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| methyl beta-D-glucopyranoside + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
| additional information | no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside | Acetivibrio thermocellus | ? | - |
? | |
| additional information | no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside | Cellvibrio japonicus | ? | - |
? | |
| additional information | the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors | Acetivibrio thermocellus | ? | - |
? | |
| additional information | the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors | Cellvibrio japonicus | ? | - |
? | |
| xylobiose + vinyl acetate | - |
Acetivibrio thermocellus | ? + vinyl alcohol | - |
? | |
| xylobiose + vinyl acetate | - |
Cellvibrio japonicus | ? + vinyl alcohol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-O-deacetylase | - |
Acetivibrio thermocellus |
| 6-O-deacetylase | - |
Cellvibrio japonicus |
| ACE | - |
Acetivibrio thermocellus |
| ACE | - |
Cellvibrio japonicus |
| Acetyl esterase | - |
Acetivibrio thermocellus |
| Acetyl esterase | - |
Cellvibrio japonicus |
| CE2 | - |
Acetivibrio thermocellus |
| CE2A | isozyme | Cellvibrio japonicus |
| CE2B | isozyme | Cellvibrio japonicus |
| CE2C | isozyme | Cellvibrio japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.6 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 241.6 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 1331 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
| 2101 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 3210 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.42 | - |
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 56.08 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 541.5 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Acetivibrio thermocellus | |
| 1356 | - |
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
| 2599 | - |
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside | isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C | Cellvibrio japonicus | |
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