3.1.1.6: acetylesterase

This is an abbreviated version!
For detailed information about acetylesterase, go to the full flat file.

Word Map on EC 3.1.1.6

Reaction

An acetic ester
+
H2O
=
an alcohol
+
acetate

Synonyms

More, Acetic ester hydrolase, Acetyl esterase, C-esterase, Chloroesterase, Citrus acetylesterase, p-nitrophenyl acetate esterase, HE, ACE, CE2, EST2, Est1, haemagglutinin-esterase, AE, GAE, geranyl acetate cleaving esterase, HerE, acetyl xylan esterase, Aes, EST-4, alpha/beta-acetylesterase, acetyl-esterase, acetic acid esterase, ragi acetic acid esterase, non-specific acetyl esterase, acetate ester-hydrolysing esterase, carbohydrate esterase, Aes1, BS2, C20orf3, 6-O-deacetylase, CE2B, CE2C, CE2A, Axe3, ESTK, TM0077, pectin acetylesterase, PAE1, CE16, carbohydrate acetylesterase, AcE1, Est24, CE16 acetyl esterase, CE16A, AE206, ybaC, acetylesterase CE16, PAE2, PAE3, PAE4, PAE5, PAE6, PAE7, PAE8, PAE9, PAE11

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.6 acetylesterase

Cloned

Cloned on EC 3.1.1.6 - acetylesterase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA of 1248 bp in pGEM-T-easy for sequencing (polymorphic residues at position 65, 282, 374, in middle European population homozygosity for R282 and R347) and subcloning, cDNA coding for residues 45-416 in pDEST15 or residues 58-416 in pGEX-1gammaT (yielding glutathione transferase-tag) for expression in Escherichia coli BL21, cDNA coding for residues 16-416 in pDEST10 for baculovirus-mediated expression in insect cell lines Sf9 and HighFive with N-terminal hexa-His-tag
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli JM110 cells
expressed in Escherichia coli Rosetta 2(DE3) cells
expressed in Escherichia coli strain DL41
-
expressed in Escherichia coli strains BL21(DE3), NiCo21(DE3), ArcticExpress and BL21-Gold(DE3)
expressed in Nicotiana tabacum
-
expressed in Pichia pastoris strain X-33
-
expression in Escherichia coli
-
expression in Escherichia coli DH5 alpha
expression of the N-terminally His-tagged enzyme in Escherichia coli BL21(DE3)
-
from genomic DNA in pT3C to generate expression plasmid pTSP-TAE and transformation of Hypocrea jecorina Rut-C30
gene ybaC, overexpression of wild-type and mutant enzymes
-
overexpression in Streptomyces lividans IAF10-164
-
overexpression of wild-type and mutant enzymes in strain BL21(DE3)
-
respiratory and enteropathogenic coronaviruses, DNA and amino acid sequence determination and analysis, transient and functional expression in COS-7 cells
-