3.1.1.6: acetylesterase

This is an abbreviated version!
For detailed information about acetylesterase, go to the full flat file.

Reaction

Synonyms

6-O-deacetylase, ACE, AcE1, acetate ester-hydrolysing esterase, acetic acid esterase, Acetic ester hydrolase, Acetyl esterase, acetyl xylan esterase, acetyl-esterase, acetylesterase CE16, AE, AE206, Aes, Aes1, alpha/beta-acetylesterase, Axe3, BS2, C-esterase, C20orf3, carbohydrate acetylesterase, carbohydrate esterase, CE16, CE16 acetyl esterase, CE16A, CE2, CE2A, CE2B, CE2C, Chloroesterase, Citrus acetylesterase, EST-4, Est1, EST2, Est24, ESTK, GAE, geranyl acetate cleaving esterase, haemagglutinin-esterase, HE, HerE, More, non-specific acetyl esterase, p-nitrophenyl acetate esterase, PAE1, PAE11, PAE2, PAE3, PAE4, PAE5, PAE6, PAE7, PAE8, PAE9, pectin acetylesterase, ragi acetic acid esterase, TM0077, ybaC

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.6 acetylesterase

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Results	in table
17	Activating Compound
123	AA Sequence
12	Application
1	CAS Registry Number
24	Cloned(Commentary)
12	Crystallization (Commentary)
66	Disease/ Diagnostics
3	Expression
12	General Information
57	Inhibitors
21	kcat/KM [mM/s]
1	Ki Value [mM]
74	KM Value [mM]
16	Localization
24	Metals/Ions
54	Molecular Weight [Da]
5	Natural Substrates/ Products (Substrates)
10	Organic Solvent Stability
99	Organism
1	Oxidation Stability
1	Pathway
52	PDB ID
28	pH Optimum
11	pH Range
5	pH Stability
8	pI Value
7	Posttranslational Modification
28	Protein Variants
52	Purification (Commentary)
3	Reaction
14	Reaction Type
92	Reference
1	Renatured (Commentary)
38	Source Tissue
82	Specific Activity [micromol/min/mg]
3	Storage Stability
297	Substrates and Products (Substrate)
48	Subunits
112	Synonyms
1	Systematic Name
28	Temperature Optimum [°C]
5	Temperature Range [°C]
16	Temperature Stability [°C]
55	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

20 - 33

2-benzylcyclohex-1-en-1-yl acetate

2 entries

63

2-ethylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

14

2-isopropylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

77

2-methylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

26 - 42

2-n-pentylcyclohex-1-en-1-yl acetate

2 entries

79

2-n-propylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

41.6

2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

Cellvibrio japonicus

-

isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

715029

76.1

2-O-acetyl-4-nitrophenyl beta-D-xylopyranoside

Thermotoga maritima

-

in 50 mM citrate-phosphate (pH 6) at 70°C

730907

7 - 17

2-t-butylcyclohex-1-en-1-yl acetate

2 entries

2101

3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

Cellvibrio japonicus

-

isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

715029

70.1

3-O-acetyl-4-nitrophenyl beta-D-xylopyranoside

Thermotoga maritima

-

in 50 mM citrate-phosphate (pH 6) at 70°C

730907

0.82 - 57.5

4-nitrophenyl acetate

8 entries

29 - 340

4-nitrophenyl butyrate

10 entries

34.5 - 48.8

4-nitrophenyl hexanoate

2 entries

41.3

4-nitrophenyl propionate

Thermotoga maritima

-

in 50 mM citrate-phosphate (pH 6) at 70°C

730907

0.15

4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside

Aspergillus oryzae

-

pH 5.5, 45°C

680967

3.23

4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside

Aspergillus oryzae

-

pH 5.5, 45°C

680967

1.9

4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside

Aspergillus oryzae

-

pH 5.5, 45°C

680967

0.91

4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside

Aspergillus oryzae

-

pH 5.5, 45°C

680967

1.77

4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside

Aspergillus oryzae

-

pH 5.5, 45°C

680967

21 - 155

4-nitrophenyl-butanoate

3 entries

241.6 - 3210

4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

3 entries

78.6

4-O-acetyl-4-nitrophenyl beta-D-xylopyranoside

Thermotoga maritima

-

in 50 mM citrate-phosphate (pH 6) at 70°C

730907

12.6

6-acetylmorphine

Rhodococcus sp.

-

recombinant enzyme, pH 8.0

652394

3

phenyl acetate

Rhodococcus sp.

-

recombinant enzyme, pH 6.4

652394

6.2 - 17.5

tributyrin

5 entries

additional information

4-nitrophenyl-beta-D-xylopyranoside monoacetate

Trichoderma reesei

A7J2C6

initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7

695758

20

2-benzylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

33

2-benzylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est II, pH 7.0, 35°C

666089

26

2-n-pentylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est II, pH 7.0, 35°C

666089

42

2-n-pentylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

7

2-t-butylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est I, pH 7.0, 35°C

666089

17

2-t-butylcyclohex-1-en-1-yl acetate

Marchantia polymorpha

-

isoenzyme Est II, pH 7.0, 35°C

666089

0.82

4-nitrophenyl acetate

Escherichia coli

A0A0A7HLG5

at pH 7.0 and 39°C

752328

5.5

4-nitrophenyl acetate

Pseudomonas putida

-

at pH 8.0 and 20°C

750119

21.8

4-nitrophenyl acetate

Talaromyces pinophilus

-

mutant enzyme S10A, at pH 5.5 and 50°C

750537

26.2

4-nitrophenyl acetate

Talaromyces pinophilus

-

mutant enzyme C16A, at pH 5.5 and 50°C

750537

36.3

4-nitrophenyl acetate

Talaromyces pinophilus

-

mutant enzyme D179A, at pH 5.5 and 50°C

750537

38.8

4-nitrophenyl acetate

Talaromyces pinophilus

-

mutant enzyme H182A, at pH 5.5 and 50°C

750537

49.2

4-nitrophenyl acetate

Talaromyces pinophilus

-

wild type enzyme, at pH 5.5 and 50°C

750537

57.5

4-nitrophenyl acetate

Thermotoga maritima

-

in 50 mM citrate-phosphate (pH 6) at 70°C

730907

29

4-nitrophenyl butyrate

Escherichia coli

P23872

mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

29

4-nitrophenyl butyrate

Escherichia coli

P23872

wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

29

4-nitrophenyl butyrate

Escherichia coli

-

wild type enzyme, in 20 mM phosphate buffer, at pH 7.1 and 30°C

729428

29.8

4-nitrophenyl butyrate

Talaromyces pinophilus

-

wild type enzyme, at pH 5.5 and 50°C

750537

37

4-nitrophenyl butyrate

Escherichia coli

-

mutant enzyme L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C

729428

78

4-nitrophenyl butyrate

Escherichia coli

P23872

mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

86

4-nitrophenyl butyrate

Escherichia coli

P23872

mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

113

4-nitrophenyl butyrate

Escherichia coli

P23872

mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

240

4-nitrophenyl butyrate

Escherichia coli

-

mutant enzyme L97F, in 20 mM phosphate buffer, at pH 7.1 and 30°C

729428

340

4-nitrophenyl butyrate

Escherichia coli

-

mutant enzyme L97F/L209F, in 20 mM phosphate buffer, at pH 7.1 and 30°C

729428

34.5

4-nitrophenyl hexanoate

Escherichia coli

-

pH 7.1, 65°C, recombinant wild-type enzyme

682718

48.8

4-nitrophenyl hexanoate

Escherichia coli

-

pH 7.1, 65°C, recombinant mutant T74A

682718

21

4-nitrophenyl-butanoate

Escherichia coli

-

pH 7.1, 25°C, recombinant wild-type enzyme

682718

145

4-nitrophenyl-butanoate

Escherichia coli

-

pH 7.1, 65°C, recombinant wild-type enzyme

682718

155

4-nitrophenyl-butanoate

Escherichia coli

-

pH 7.1, 65°C, recombinant mutant T74A

682718

241.6

4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

Cellvibrio japonicus

-

isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

715029

1331

4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

Acetivibrio thermocellus

-

isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

715029

3210

4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside

Cellvibrio japonicus

-

isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C

715029

6.2

tributyrin

Escherichia coli

P23872

wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

6.9

tributyrin

Escherichia coli

P23872

mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

9.3

tributyrin

Escherichia coli

P23872

mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

17.4

tributyrin

Escherichia coli

P23872

mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950

17.5

tributyrin

Escherichia coli

P23872

mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C

715950