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3.1.1.6: acetylesterase

This is an abbreviated version!
For detailed information about acetylesterase, go to the full flat file.

Word Map on EC 3.1.1.6

Reaction

An acetic ester
+
H2O
=
an alcohol
 +
acetate

Synonyms

6-O-deacetylase, ACE, AcE1, acetate ester-hydrolysing esterase, acetic acid esterase, Acetic ester hydrolase, Acetyl esterase, acetyl xylan esterase, acetyl-esterase, acetylesterase CE16, AE, AE206, Aes, Aes1, alpha/beta-acetylesterase, Axe3, BS2, C-esterase, C20orf3, carbohydrate acetylesterase, carbohydrate esterase, CE16, CE16 acetyl esterase, CE16A, CE2, CE2A, CE2B, CE2C, Chloroesterase, Citrus acetylesterase, EST-4, Est1, EST2, Est24, ESTK, GAE, geranyl acetate cleaving esterase, haemagglutinin-esterase, HE, HerE, More, non-specific acetyl esterase, p-nitrophenyl acetate esterase, PAE1, PAE11, PAE2, PAE3, PAE4, PAE5, PAE6, PAE7, PAE8, PAE9, pectin acetylesterase, ragi acetic acid esterase, TM0077, ybaC

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.6 acetylesterase

Temperature Stability

Temperature Stability on EC 3.1.1.6 - acetylesterase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
the enzyme is not stable at 100°C, resulting in a half-life of less than 5 min
30 - 40
-
stable
35 - 51
the enzyme maintains more than 80% activity between 35 and 51°C
37
-
40 min, the transiently expressed respiratory viruses show significantly reduced activity, while the transiently expressed enteropathogenic viruses are stable
40
-
pH 5-6, 24 h stable
40 - 60
-
decreasing stability, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, pH 7, 1 h
45
-
the deacetylation activity is quite thermostable in the range of 20-45°C, suffering a sharp decrease above 50?C
50
-
75% activity, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, pH 7, 1 h
55
-
the wild type enzyme shows a half-life of 40 min at 55°C
61
-
denaturation of Aes mutant V20D, thermal stability analysis at 20-90°C, differential scanning calorimetry and circular dichroism measurements, the irreversible inactivation process is more complex than a two-state transition
68
-
denaturation of Aes, thermal stability analysis at 20-90°C, differential scanning calorimetry and circular dichroism measurements, the irreversible inactivation process is more complex than a two-state transition