Activating Compound | Comment | Organism | Structure |
---|---|---|---|
benzethonium chloride | i.e. (diisobutyl phenoxyethoxyethyl) dimethyl benzylammonium chloride, up to 2.5fold enhancement of activity | Acinetobacter sp. | |
bis(2-ethylhexyl) sodium sulfosuccinate | 138% of initial activity | Acinetobacter sp. | |
bis(octadecyl) ammonium chloride | 246% of initial activity | Acinetobacter sp. | |
cetyltrimethyl ammonium bromide | 184% of initial activity | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Acinetobacter sp. | |
Fe3+ | - |
Acinetobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32.5 | - |
p-nitrophenyl acetate | pH 7.0, 25°C, presence of benzethonium chloride | Acinetobacter sp. | |
385 | - |
p-nitrophenyl acetate | pH 7.0, 25°C, absence of benzethonium chloride | Acinetobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | stimulation | Acinetobacter sp. | |
Ba2+ | stimulation | Acinetobacter sp. | |
Ca2+ | up to 150% stimulation | Acinetobacter sp. | |
Co2+ | stimulation | Acinetobacter sp. | |
Mg2+ | stimulation | Acinetobacter sp. | |
Mn2+ | stimulation | Acinetobacter sp. | |
Pb2+ | stimulation | Acinetobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE | Acinetobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Acinetobacter sp. CGMCC 0789 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.082 | - |
substrate p-nitrophenyl laurate, pH 7.0, 25°C | Acinetobacter sp. |
0.251 | - |
substrate p-nitrophenyl acetate, pH 7.0, 25°C | Acinetobacter sp. |
0.297 | - |
substrate p-nitrophenyl decanoate, pH 7.0, 25°C | Acinetobacter sp. |
0.352 | - |
substrate p-nitrophenyl hexanoate, pH 7.0, 25°C | Acinetobacter sp. |
0.369 | - |
substrate p-nitrophenyl propionate, pH 7.0, 25°C | Acinetobacter sp. |
0.474 | - |
substrate p-nitrophenyl butyrate, pH 7.0, 25°C | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O | highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride | Acinetobacter sp. | ? | - |
? | |
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O | highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride | Acinetobacter sp. CGMCC 0789 | ? | - |
? | |
p-nitrophenyl acetate + H2O | - |
Acinetobacter sp. | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl acetate + H2O | - |
Acinetobacter sp. CGMCC 0789 | p-nitrophenol + acetate | - |
? | |
p-nitrophenyl butyrate + H2O | - |
Acinetobacter sp. | p-nitrophenol + butyrate | - |
? | |
p-nitrophenyl decanoate + H2O | - |
Acinetobacter sp. | p-nitrophenol + decanoate | - |
? | |
p-nitrophenyl hexanoate + H2O | - |
Acinetobacter sp. | p-nitrophenol + hexanoate | - |
? | |
p-nitrophenyl hexanoate + H2O | - |
Acinetobacter sp. CGMCC 0789 | p-nitrophenol + hexanoate | - |
? | |
p-nitrophenyl laurate + H2O | - |
Acinetobacter sp. | p-nitrophenol + laurate | - |
? | |
p-nitrophenyl laurate + H2O | - |
Acinetobacter sp. CGMCC 0789 | p-nitrophenol + laurate | - |
? | |
p-nitrophenyl propionate + H2O | - |
Acinetobacter sp. | p-nitrophenol + propionate | - |
? | |
p-nitrophenyl propionate + H2O | - |
Acinetobacter sp. CGMCC 0789 | p-nitrophenol + propionate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 20000, SDS-PAGE | Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, 60% residual activity | Acinetobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Acinetobacter sp. |