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Literature summary for 3.1.1.6 extracted from
- Purification and characterization of acetyl esterase from Candida guilliermondii (2000), Lett. Appl. Microbiol., 30, 167-171.
Application
| Application | Comment | Organism |
|---|---|---|
| paper production | the enzyme can be a potential source for pretreatment of pulps and biobleaching in paper manufacture due to its activity with acetylated xylan leading to complete hydrolysis | Meyerozyma guilliermondii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.63 | - |
1-acetoxynaphthalene | pH 7.8, 45°C | Meyerozyma guilliermondii |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Meyerozyma guilliermondii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Meyerozyma guilliermondii |
| 67000 | - |
1 * 67000, SDS-PAGE | Meyerozyma guilliermondii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meyerozyma guilliermondii | - |
- |
- |
| Meyerozyma guilliermondii NRRL Y-17257 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 8.6fold, to homogeneity | Meyerozyma guilliermondii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 71.4 | - |
purified enzyme | Meyerozyma guilliermondii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl acetate + H2O | - |
Meyerozyma guilliermondii | 1-naphthol + acetate | - |
? | |
| 1-naphthyl acetate + H2O | - |
Meyerozyma guilliermondii NRRL Y-17257 | 1-naphthol + acetate | - |
? | |
| acetylxylan + H2O | - |
Meyerozyma guilliermondii | ? | - |
? | |
| acetylxylan + H2O | - |
Meyerozyma guilliermondii NRRL Y-17257 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67000, SDS-PAGE | Meyerozyma guilliermondii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Acetyl esterase | - |
Meyerozyma guilliermondii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Meyerozyma guilliermondii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Meyerozyma guilliermondii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Meyerozyma guilliermondii |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Meyerozyma guilliermondii | isoelectric focusing | - |
7.6 |
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Release 2023.1 (January 2023)
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