Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.6 extracted from

  • Puchart, V.; Vrsanska, M.; Mastihubova, M.; Topakas, E.; Vafiadi, C.; Faulds, C.B.; Tenkanen, M.; Christakopoulos, P.; Biely, P.
    Substrate and positional specificity of feruloyl esterases for monoferuloylated and monoacetylated 4-nitrophenyl glycosides (2007), J. Biotechnol., 127, 235-243.
    View publication on PubMed
Search for more literature for 3.1.1.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.28
-
 4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae
6.78
-
 4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae
9.44
-
 4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae
16.4
-
 4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C Aspergillus oryzae
44.6
-
 4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity by SDS-PAGE and isoelectric focusing Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus oryzae 4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
 ?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O preferred substrate Aspergillus oryzae 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O
-
 Aspergillus oryzae 4-nitrophenyl-beta-D-xylopyranoside + acetate
-
 ?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus oryzae 4-nitrophenyl-alpha-L-arabinofuranoside + acetate
-
 ?
additional information the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters Aspergillus oryzae ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the carbohydrate esterase family 1 Aspergillus oryzae
non-specific acetyl esterase
-
 Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C Aspergillus oryzae
0.91
-
 4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae
1.77
-
 4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside pH 5.5, 45°C Aspergillus oryzae
1.9
-
 4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae
3.23
-
 4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside pH 5.5, 45°C Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 6
-
 Aspergillus oryzae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus oryzae isoelectric focusing
-
 3.6