Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain X-33 | Podospora anserina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Podospora anserina | - |
- |
- |
Podospora anserina DSM 980 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal-ion affinity chromatography and Superdex 200 gel filtration | Podospora anserina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00507 | - |
with 4-nitrophenyl butyrate as substrate, at pH 6.0 and 35°C | Podospora anserina |
2.37 | - |
with 4-nitrophenyl propionate as substrate, at pH 6.0 and 35°C | Podospora anserina |
5.64 | - |
with 4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C | Podospora anserina |
10.3 | - |
with 4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C | Podospora anserina |
13.3 | - |
with 4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside as substrate, at pH 6.0 and 35°C | Podospora anserina |
104.85 | - |
with 4-nitrophenyl acetate as substrate, at pH 6.0 and 35°C | Podospora anserina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
4-nitrophenyl 2-O-acetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina DSM 980 | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
4-nitrophenyl 3-O-acetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
4-nitrophenyl 4-O-acetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina | 4-nitrophenyl-beta-D-xylopyranoside + acetate | - |
? | |
4-nitrophenyl acetate + H2O | highest activity | Podospora anserina | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | highest activity | Podospora anserina DSM 980 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | lowest activity | Podospora anserina | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | lowest activity | Podospora anserina DSM 980 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Podospora anserina | 4-nitrophenol + propionate | - |
? | |
4-nitrophenyl propionate + H2O | - |
Podospora anserina DSM 980 | 4-nitrophenol + propionate | - |
? | |
acetylglucuronoxylan + H2O | the enzyme deacetylates singly and doubly acetylated xylopyranosyl residues in the polymer to such an extent that it results in the polymer precipitation | Podospora anserina | glucuronoxylan + acetate | - |
? | |
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + 3 H2O | all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h | Podospora anserina | methyl beta-D-xylopyranoside + 3 acetate | - |
? | |
methyl 2,3,4-O-triacetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina | methyl 3,4-O-diacetyl-beta-D-xylopyranoside + acetate | - |
? | |
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + 2 H2O | all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h | Podospora anserina | methyl beta-D-xylopyranoside + 2 acetate | - |
? | |
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O | - |
Podospora anserina | methyl 2-O-acetyl-beta-D-xylopyranoside + acetate | - |
? | |
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O | all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h | Podospora anserina | methyl beta-D-xylopyranoside + 2 acetate | - |
? | |
methyl 2-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O | - |
Podospora anserina | methyl xylotriose + acetate | - |
? | |
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + 2 H2O | all four acetylated derivatives of methyl beta-xylopyranoside are completely deacetylated after 24 h | Podospora anserina | methyl beta-D-xylopyranoside + 2 acetate | - |
? | |
methyl 3-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O | - |
Podospora anserina | methyl xylotriose + acetate | - |
? | |
methyl 4-O-acetyl-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->4)-beta-D-xylopyranoside + H2O | - |
Podospora anserina | methyl xylotriose + acetate | - |
? | |
additional information | the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2 | Podospora anserina | ? | - |
? | |
additional information | the enzyme does not attack the 3-O-acetyl group of xylopyranosyl residues carrying 4-O-methyl-D-glucuronic acid at position 2 | Podospora anserina DSM 980 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Podospora anserina |
? | x * 38400, calculated from amino acid sequence | Podospora anserina |
Synonyms | Comment | Organism |
---|---|---|
CE16 acetyl esterase | - |
Podospora anserina |
CE16A | - |
Podospora anserina |