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Literature summary for 3.1.1.6 extracted from
- Specificity of an Aspergillus niger esterase deacetylating cellulose acetate (2003), Cellulose, 10, 85-95.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.1 | - |
Cellulose acetate | pH 5.0, 45°C | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from the crude commercial enzyme preparation, 27fold | Aspergillus niger |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Aspergillus niger | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Aspergillus niger |
| 16050 | - |
purified enzyme | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl xylan + H2O | from beech wood or oat spelt | Aspergillus niger | xylan + acetate | - |
? |  |
| cellobiose octaacetate + H2O | low activity | Aspergillus niger | ? | - |
? | |
| cellulose acetate + H2O | also in form of acetylated starch, water-soluble and water-insoluble substrate, specific for acetyl substituents at the C2- and C3-positions, C6-acetyl is not hydrolyzed | Aspergillus niger | cellulose + acetate | - |
? | |
| glucose pentaacetate + H2O | good substrate | Aspergillus niger | ? | - |
? | |
| additional information | substrate specificity and regioselectivity, deacetylation activity of the enzyme is not restricted to polymers with beta-1,4 glycosidic bonds, no activity with cellulose sulfate, the enzyme enhances endoglucanase, but is itself not influenced by endoglucanase | Aspergillus niger | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Acetyl esterase | - |
Aspergillus niger |
| AE | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | below, isoelectric focusing | - |
3 |
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Release 2023.1 (January 2023)
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