KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.76 | - |
4-nitrophenyl acetate | at pH 8.0 and 35°C | Lichtheimia corymbifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, SDS-PAGE | Lichtheimia corymbifera |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lichtheimia corymbifera | - |
- |
- |
Lichtheimia corymbifera AS2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, phenyl Sepharose column chromatography, ceramic hydroxy-apatite column chromatography, and Resource Q column chromatography | Lichtheimia corymbifera |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.52 | - |
crude enzyme, at pH 8.0 and 35°C | Lichtheimia corymbifera |
221.9 | - |
after 423.39fold purification, at pH 8.0 and 35°C | Lichtheimia corymbifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Lichtheimia corymbifera | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | - |
Lichtheimia corymbifera AS2 | 4-nitrophenol + acetate | - |
? | |
astragaloside I + H2O | - |
Lichtheimia corymbifera | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
astragaloside I + H2O | - |
Lichtheimia corymbifera AS2 | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
astragaloside II + H2O | - |
Lichtheimia corymbifera | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
astragaloside II + H2O | - |
Lichtheimia corymbifera AS2 | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
isoastragaloside I + H2O | - |
Lichtheimia corymbifera | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
isoastragaloside I + H2O | - |
Lichtheimia corymbifera AS2 | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
isoastragaloside II + H2O | - |
Lichtheimia corymbifera | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? | |
isoastragaloside II + H2O | - |
Lichtheimia corymbifera AS2 | astragaloside IV + ? | i.e. 3-O-beta-D-xylopyranosyl-6-O-beta-D-glucopyranosyl-cycloastragenol | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, SDS-PAGE | Lichtheimia corymbifera |
Synonyms | Comment | Organism |
---|---|---|
Acetyl esterase | - |
Lichtheimia corymbifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Lichtheimia corymbifera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | within the temperature range of 30-45°C, the enzyme exhibits more than 95% of optimum activity, whereas at temperatures higher than 45°C, the activity sharply declines | Lichtheimia corymbifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the deacetylation activity is quite thermostable in the range of 20-45°C, suffering a sharp decrease above 50?C | Lichtheimia corymbifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lichtheimia corymbifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | the enzyme retains about 90% and 88% of its maximal activity at pH 7.0 and 8.5, respectively | Lichtheimia corymbifera |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | the enzyme is quite pH-stable over a broad range of pH 7.0-9.5, exhibiting a sharp decrease in activity below pH 4.5 | Lichtheimia corymbifera |