Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.6 extracted from

  • Shimoda, K.; Kubota, N.; Hirata, T.; Kawano, T.
    Asymmetric transformation of enol acetates with esterases from Marchantia polymorpha (2004), J. Mol. Catal. B, 29, 123-127.
No PubMed abstract available
Search for more literature for 3.1.1.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
0.09
-
 2-methylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
0.11
-
 2-ethylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
0.3
-
 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
0.54
-
 2-n-propylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
0.72
-
 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
0.97
-
 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
1.87
-
 2-isopropylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
2.01
-
 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
2.54
-
 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22500
-
 2 * 27000, isoenzyme EstI, 2 * 22500, isoenzyme Est II, SDS-PAGE Marchantia polymorpha
27000
-
 2 * 27000, isoenzyme EstI, 2 * 22500, isoenzyme Est II, SDS-PAGE Marchantia polymorpha
45000
-
 gel filtration, isoenzyme Est II Marchantia polymorpha
54000
-
 gel filtration, isoenzyme Est I Marchantia polymorpha

Organism

Organism UniProt Comment Textmining
Marchantia polymorpha
-
 two isoenzymes
-

Purification (Commentary)

Purification (Comment) Organism
isoenzymes Est I and Est II Marchantia polymorpha

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-benzylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product Marchantia polymorpha 2-benzylcyclohexanone + acetate
-
 ?
2-benzylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est II, 11% conversion of substrate with R-configuration of product Marchantia polymorpha 2-benzylcyclohexanone + acetate
-
 ?
2-ethylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product Marchantia polymorpha 2-ethylcyclohexanone + acetate
-
 ?
2-isopropylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 10% conversion of substrate with R-configuration of product Marchantia polymorpha 2-isopropylcyclohexanone + acetate
-
 ?
2-methylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product Marchantia polymorpha (S)-2-methylcyclohexanone + acetate
-
 ?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 20% conversion of substrate with R-configuration of product Marchantia polymorpha 2-n-pentylcyclohexanone + acetate
-
 ?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est II, 16% conversion of substrate with S-configuration of product Marchantia polymorpha 2-n-pentylcyclohexanone + acetate
-
 ?
2-n-propylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product Marchantia polymorpha 2-n-propylcyclohexanone + acetate
-
 ?
2-t-butylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est I, 21% conversion of substrate with S-configuration of product Marchantia polymorpha 2-t-butylcyclohexanone + acetate
-
 ?
2-t-butylcyclohex-1-en-1-yl acetate + H2O isoenzyme Est II, 20% conversion of substrate with R-configuration of product Marchantia polymorpha 2-t-butylcyclohexanone + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 27000, isoenzyme EstI, 2 * 22500, isoenzyme Est II, SDS-PAGE Marchantia polymorpha

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
14
-
 2-isopropylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
17
-
 2-t-butylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
20
-
 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
26
-
 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
33
-
 2-benzylcyclohex-1-en-1-yl acetate isoenzyme Est II, pH 7.0, 35°C Marchantia polymorpha
42
-
 2-n-pentylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
63
-
 2-ethylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
77
-
 2-methylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha
79
-
 2-n-propylcyclohex-1-en-1-yl acetate isoenzyme Est I, pH 7.0, 35°C Marchantia polymorpha