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3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Word Map on EC 3.1.1.20

Reaction

digallate
+
H2O
= 2 gallate

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.20 tannase

Purification

Purification on EC 3.1.1.20 - tannase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1910fold to apparent homogeneity
-
2 isoenzymes
-
acetone precipitation
-
acetone precipitation and G25 Sephadex gel filtration
-
ammonium sulfate precipitation
ammonium sulfate precipitation and DEAE cellulose column chromatography
-
ammonium sulfate precipitation and DEAE-cellulose column chromatography (overall purification of 39.74fold with a yield of 19.29%)
-
ammonium sulfate precipitation and ultrafiltration
-
ammonium sulfate precipitation followed by Sephadex G-75 gel filtration
-
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-100 gel filtration
ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and Mono-Q column chromatography
ammonium sulfate precipitation, Q-Sepharose column chromatography, hydroxylapatite column chromatography, and MonoQ column chromatography
-
aquaeous two-phase extraction with 17% (w/v) sodium citrate and 18.18% (w/v) PEG 1000 at pH 7.0
-
best at pH 5.5, overview, 51fold
-
by ammonium sulfate precipitation followed by ion-exchange chromatography, 19.5folf with 13.5% yield, to homogeneity
-
DEAE-cellulose chromatography followed by Sephadex G-100 gel filtration
-
DEAE-cellulose column chromatography and Sephacryl S-200 gel filtration
-
DEAE-Sepharose column chromatography and Q-Sepharose column chromatography
-
endogenous and recombinant tannases purified by ion-exchange chromatography and gel filtration
evaluation of influence of PEG concentration, molar mass, pH, and citrate concentrations on aqueous biphasic systems based on 24 factorial designs and their capacity for purification of tannase secreted by Aspergillus tamarii strain URM 7115. Purification is best through a biphasic system composed of 600 g/mol MPEG, 24% w/w CPEG, 15% w/w CCIT, at pH 6.0, and resulting in 6.33 enzyme partition, 131.25% yield, 19.80 purification factor and 195.08 selectivity
-
intracellular tannase partially
-
isoelectric focusing, High Q column chromatography, High Trap Q column chromatography, High S column chromatography, and Superdex G-200 gel filtration
-
native enzyme 135fold by ultrafiltration using 100 kDa molecular weight cut off and gel filtration
-
native enzyme 24.21fold by ammonium sulfate fractionation and gel filtration
-
native enzyme by acetone precipitation and anion exchange chromatography
native enzyme by ammonium sulfate fractionation, SDS-PAGE, and anion exchange chromatography 19.3fold to homogeneity
-
native enzyme from production in a bioreactor by organic solvent precipitation and anion exchange chromatography
-
native enzyme partially 10fold by ammonium sulfate precipitation followed by anion exchange chromatography
-
native extracellular enzyme 4.9fold from submerged fermentation broth by anion exchange chromatography, dialysis, and gel filtration, followed by another step of dialysis
-
native extracellular enzyme partially about 2fold from fermentation broth by ammonium sulfate fractionation and dialysis
-
native intracellular enzyme 36.7fold from submerged culture by ammonium sulfate fractionation, dialysis and desalting gel filtration, and two steps of gel filtration
native isozymes TAH I and TAH II 7.9fold and 10.5fold, respectively, to homogeneity
-
Ni-NTA His Bind column chromatography
Ni-NTA His-Bind resin column chromatography
-
Ni2+ affinity column chromatography
nickel-affinity HisTrap column chromatography and Superdex 200 gel filtration
on affinity column
-
partial purification by ammonium sulfate precipitation and aqueous two phase extraction with PEG6000
-
partial purification by HiTrap G25 gel filtration (5.4fold purification and 5.6fold concentration with a recovery yield higher than 90%)
-
partially
-
partially purified by acetone precipitation, 2.33fold with 79.06% recovery
Hyalopus sp.
-
partially purified by DEAE-Sepharose column chromatography (21times with 62.5% recovery)
-
poorly activated Ni-IDA 6% agarose gels column chromatography
-
purified from the strain AO1
-
recombinant extracellular enzyme 1.3fold from Aspergillus niger strain Bdel4 culture medium by desalting gel filtration, and gel filtration
recombinant extracellular enzyme from Pichia pastoris strain KM71 culture supernatant by anion exchange chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
recombinant secreted enzyme from Pichia pastoris strain GS115 by anion exchange chromatography and gel filtration
recombinant wild-type and mutant enzymes from Pichia pastoris strain GS115 by anion exchange chromatography, ultrafiltration, and gel filtration
Sephadex G-200 gel filtration
-
TALON metal affinity resin column chromatography
Talon Superflow resin column chromatography
-
to apparent homogeneity, by ultrafiltration, anion-exchange chromatography and gel filtration, with a final yield of 0.3%, 46fold
-
to electrophoretic homogeneity through two-step column chromatography
-
to homogeneity from culture supernatant
-
TSKgel DEAE-5PW column chromatography and G3000-SWXL gel filtration
-
ultrafiltration and Sephadex G-200 gel filtration