Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heptane | at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 97% after 6 min | Aspergillus niger | |
additional information | Tween 20 and Triton X-100 have no effect | Aspergillus niger | |
petroleum ether | at 20% of concentration increases activity by 19% after 60 min, and at 60% of concentration by 73% after 6 min | Aspergillus niger | |
Tween 80 | at 0.1% of concentration increases activity by 25% after 60 min | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetone | at 60% of concentration, completely inhibits activity at 30°C | Aspergillus niger | |
Cu2+ | ca. 20% inhibition after 60 min of incubation at 30°C and pH 5 | Aspergillus niger | |
EDTA | at 0.01% of concentration 22% inhibition after 5 min and at 0.1% of concentration 19% inhibition after 60 min at 30°C | Aspergillus niger | |
ethanol | at 60% of concentration, completely inhibits activity at 30°C | Aspergillus niger | |
Fe3+ | more than 40% inhibition after 5 min and 60 min of incubation at 30°C and pH 5 | Aspergillus niger | |
formaldehyde | 51.5% inhibition at 20% after 60 min at 30°C | Aspergillus niger | |
heptane | at 60% of concentration 95% inhibition after 60 min at 30°C | Aspergillus niger | |
Mg2+ | ca. 20% inhibition after 5 min of incubation at 30°C and pH 5 | Aspergillus niger | |
Mn2+ | ca. 20% inhibition after 5 min of incubation at 30°C and pH 5 | Aspergillus niger | |
additional information | at 0.1% and 0.01% of concentration, Ca2+, Tween 20 and Triton X-100 have no effect at 30°C | Aspergillus niger | |
phenylmethylsulfonyl fluoride | 38% inhibition after 5 min of incubation at 30°C and pH 5, indicating the presence of a serine or cysteine residue in the catalytic site | Aspergillus niger | |
SDS | at 0.01% of concentration 15% inhibition after 60 min at 30°C | Aspergillus niger | |
tetrahydrofuran | at 60% of concentration, completely inhibits activity at 30°C | Aspergillus niger | |
Tween 80 | at 0.01% of concentration 35% inhibition after 5 min at 30°C | Aspergillus niger | |
Zn2+ | ca. 10% inhibition after 5 min and 60 min of incubation at 30°C and pH 5 | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
Tannic acid | at 60°C | Aspergillus niger | |
0.41 | - |
Methyl 3,4,5-trihydroxybenzoate | at 60°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE | Aspergillus niger |
75000 | - |
1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE | Aspergillus niger |
100000 | - |
1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger GH1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity, by ultrafiltration, anion-exchange chromatography and gel filtration, with a final yield of 0.3%, 46fold | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude extract initially has a specific activity of 5.2 IU/mg. The 46fold purified enzyme has a specific activity of 238.14 IU/mg | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl 3,4,5-trihydroxybenzoate + H2O | - |
Aspergillus niger | methanol + 3,4,5-trihydroxybenzoate | - |
? | |
methyl 3,4,5-trihydroxybenzoate + H2O | - |
Aspergillus niger GH1 | methanol + 3,4,5-trihydroxybenzoate | - |
? | |
tannic acid + H2O | - |
Aspergillus niger | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus niger GH1 | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 50000 + 1 * 75000 + 1 * 100000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
tannase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
with 0.1 M citrate buffer, pH 5.5 | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | at 20°C exhibits a relative activity of 10% | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | half-lives of the enzyme at 50, 60, and 70°C are 281, 25, and 4 min, respectively | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
at 30°C, using methyl 3,4,5-trihydroxybenzoate as substrate | Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | at pH 2.0 exhibits a relative activity of about 45% | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | in the pH range 3.0-5.0, tannase proves to be significantly stable, showing half-lives ranging from 131 to 372 h. At pH 6.0 and 5.5 half-lives of 632 h and 607 h, respectively | Aspergillus niger |