Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Aspergillus foetidus | |
Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Rhizopus arrhizus | |
Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Aspergillus foetidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Aspergillus foetidus | |
1,10-phenanthroline | - |
Rhizopus arrhizus | |
2-mercaptoethanol | - |
Aspergillus foetidus | |
2-mercaptoethanol | - |
Rhizopus arrhizus | |
Ba2+ | - |
Aspergillus foetidus | |
Ba2+ | - |
Rhizopus arrhizus | |
Ca2+ | - |
Aspergillus foetidus | |
Ca2+ | - |
Rhizopus arrhizus | |
Cu2+ | - |
Aspergillus foetidus | |
Cu2+ | - |
Rhizopus arrhizus | |
EDTA | - |
Aspergillus foetidus | |
EDTA | - |
Rhizopus arrhizus | |
Fe3+ | - |
Aspergillus foetidus | |
Fe3+ | - |
Rhizopus arrhizus | |
Hg2+ | - |
Aspergillus foetidus | |
Hg2+ | - |
Rhizopus arrhizus | |
Mn2+ | - |
Aspergillus foetidus | |
Mn2+ | - |
Rhizopus arrhizus | |
Triton X-100 | - |
Aspergillus foetidus | |
Triton X-100 | - |
Rhizopus arrhizus | |
Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Aspergillus foetidus | |
Urea | slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M | Rhizopus arrhizus | |
Zn2+ | - |
Aspergillus foetidus | |
Zn2+ | - |
Rhizopus arrhizus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
Tannic acid | pH 5.0, 40°C | Rhizopus arrhizus | |
0.21 | - |
Tannic acid | pH 5.0, 40°C | Aspergillus foetidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Rhizopus arrhizus | - |
gallate + ? | - |
? | |
tannic acid + H2O | Aspergillus foetidus | - |
gallate + ? | - |
? | |
tannic acid + H2O | Rhizopus arrhizus RO IIT RB-13 | - |
gallate + ? | - |
? | |
tannic acid + H2O | Aspergillus foetidus GMRB 013 MTCC 3557 | - |
gallate + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus foetidus | - |
- |
- |
Aspergillus foetidus GMRB 013 MTCC 3557 | - |
- |
- |
Rhizopus arrhizus | - |
- |
- |
Rhizopus arrhizus RO IIT RB-13 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by acetone precipitation and anion exchange chromatography | Rhizopus arrhizus |
native enzyme by acetone precipitation and anion exchange chromatography | Aspergillus foetidus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | co-culture with Aspergillus foetidus, solid-state fermentation on tannin rich substrates | Rhizopus arrhizus | - |
additional information | co-culture with Rhizopus oryzae, solid-state fermentation on tannin rich substrates | Aspergillus foetidus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | - |
Rhizopus arrhizus | gallate + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus foetidus | gallate + ? | - |
? | |
tannic acid + H2O | - |
Rhizopus arrhizus RO IIT RB-13 | gallate + ? | - |
? | |
tannic acid + H2O | - |
Aspergillus foetidus GMRB 013 MTCC 3557 | gallate + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
enzyme from co-culture with Aspergillus foetidus | Rhizopus arrhizus |
40 | - |
enzyme from co-culture with Rhizopus oryzae | Aspergillus foetidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 55 | activity range | Rhizopus arrhizus |
5 | 55 | activity range | Aspergillus foetidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivation of purified enzyme after 255 min | Rhizopus arrhizus |
40 | - |
inactivation of purified enzyme after 255 min | Aspergillus foetidus |
60 | - |
inactivation of purified enzyme after 105 min | Rhizopus arrhizus |
60 | - |
inactivation of purified enzyme after 105 min | Aspergillus foetidus |
65 | - |
inactivation of purified enzyme after 75 min | Rhizopus arrhizus |
65 | - |
inactivation of purified enzyme after 75 min | Aspergillus foetidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
enzyme from co-culture with Aspergillus foetidus, the tannase needs an acidic environment to be active | Rhizopus arrhizus |
5 | - |
enzyme from co-culture with Rhizopus oryzae, the tannase needs an acidic environment to be active | Aspergillus foetidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | activity range | Rhizopus arrhizus |
3 | 6 | activity range | Aspergillus foetidus |