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1,2-dihydroxybenzene
-
competitive
1,3-dihydroxybenzene
-
competitive
1,4-dihydroxybenzene
-
competitive
1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol
2,3-Dihydroxybenzoic acid
-
competitive
2,5-dihydroxybenzoic acid
-
competitive
2,6-dihydroxybenzoic acid
-
noncompetitive
2-hydroxybenzoic acid
-
competitive
3,4-dihydroxybenzoic acid
-
competitive
3,5-Dihydroxybenzoic acid
-
competitive
3-Hydroxybenzoic acid
-
competitive
4-hydroxybenzoic acid
-
competitive
8-hydroxyquinoline
-
47.2% residual activity at 1 mM
acetic acid
-
completely inhibits activity
AgNO3
-
98.77% residual activity at 1 mM
alpha-glutathione
-
inhibits both isozymes TAH I and TAH II
Benzene
-
98% residual activity at 5% (v/v)
benzoic acid
-
83.1% residual activity at 1 mM
carbon tetrachloride
-
complete inactivation
chloroform
-
completely inhibits activity
Cr2+
52% residual activity at 1 mM
cysteine
-
38% inhibition at 1 mM
D-glucose
-
slightly stimulating, inhibits intracellular enzyme production at concentrations above 0.05% w/v, and extracellular enzyme production above 0.1% w/v
diisopropylfluorophosphate
gallate
-
inhibits the enzymatic hydrolysis of propyl gallate
H2O2
H2O2 concentrations of less than 2% and higher than 10% lead to a decline in enzyme activity
iso-amylalcohol
inhibits by 35% at 20%
Isoamylalcohol
-
completely inhibits activity
Isopropyl alcohol
-
completely inhibits activity
Li+
3.07% residual activity at 20 mM
mercuribenzoic acid
-
48.8% residual activity at 1 mM
mercuric benzoic acid
-
-
Mo2+
22.48% residual activity at 20 mM
phenyl methyl sulphonyl fluoride
95% inhibition, at 30°C in sodium citrate buffer, pH 6.0
phenylmethylsulfonyl fluoride
sodium dodecyl sulphate
-
inhibits isozyme TAH II
soybean extract
-
inhibits at 0.05-1.0% w/v
-
Triton X 100
-
1 mM inhibits activity by 20%
Tween
-
1 mM inhibits activity by 20%
Tween 40
-
activation up to 0.05% v/v, complete inhibition at 0.6% v/v; activation up to 0.4% v/v, complete inhibition at 0.6% v/v
Tween-60
-
complete inactivation
Tween-80
-
complete inactivation
1,10-phenanthroline
-
-
1,10-phenanthroline
-
42% inhibition at 1 mM
1,10-phenanthroline
-
slight inhibition, 1 mM, 88% remaining activity
1,10-phenanthroline
-
40% residual activity at 1 mM
1-propanol
-
activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme
1-propanol
-
inhibits the enzymatic hydrolysis of propyl gallate
1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol
-
-
1-[(naphthalen-1-yl)oxy]-3-[(propan-2-yl)amino]propan-2-ol
-
49.6% residual activity at 1 mM
2-mercaptoethanol
-
-
2-mercaptoethanol
-
at 1 mM
2-mercaptoethanol
-
inhibits both isozymes TAH I and TAH II
2-mercaptoethanol
-
39% inhibition at 1 mM
2-mercaptoethanol
-
41% inhibition at 1 mM
2-mercaptoethanol
-
1 mM, 68.3% remaining activity
2-mercaptoethanol
complete inhibition at 1%
2-mercaptoethanol
-
16% residual activity at 1 mM
4-Aminobenzoic acid
-
81.9% residual activity at 1 mM
4-Aminobenzoic acid
-
25% inhibition at 1 mM
4-chloromercuribenzoate
-
inhibits both isozymes TAH I and TAH II
4-chloromercuribenzoate
-
-
acetone
-
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01%
acetone
-
at 60% of concentration, completely inhibits activity at 30°C
acetone
-
complete inactivation
Ag+
-
57% residual activity at 5 mM
Ag+
-
inhibits both isozymes TAH I and TAH II
Ag+
-
slight competitive inhibition at 1 mM, 82.4% remaining activity
Ag+
51% residual activity at 1 mM
Al3+
complete inhibition at 1 mM
Al3+
-
75.14% residual activity at 1 mM
Al3+
97% residual activity at 1 mM
Ba2+
complete inhibition at 5 mM
Ba2+
-
competitive inhibitor
Ba2+
-
27% inhibition at 1 mM
Ba2+
-
competitive inhibition at 1 mM, 5.4% remaining activity
beta-mercaptoethanol
-
beta-mercaptoethanol
-
59.4% residual activity at 1 mM
beta-mercaptoethanol
-
37.07% residual activity at 20 mM
beta-mercaptoethanol
-
1 mM inhibits activity by 85%
beta-mercaptoethanol
-
about 10% residual activity at 1 mM
beta-mercaptoethanol
63% residual activity at 1 mM
Bromoacetic acid
-
-
Bromoacetic acid
-
53.3% residual activity at 1 mM
Ca2+
0.84% residual activity at 20 mM
Ca2+
-
58% inhibition at 20 mM, noncompetitive
Ca2+
-
7% inhibition of tannase produced under submerged fermentation at 1 mM
Ca2+
-
88.7% residual activity at 1 mM
Ca2+
-
92.3% residual activity at 1 mM
Ca2+
98.3% residual activity at 1 mM
Ca2+
-
slight competitive inhibition at 1 mM, 86.6% remaining activity
Cd2+
11.82% residual activity at 20 mM
Cd2+
-
57% residual activity at 5 mM
Cd2+
-
55% inhibition at 20 mM, noncompetitive
Cd2+
45% inhibition, at 30°C in sodium citrate buffer, pH 6.0
Cd2+
-
inhibits both isozymes TAH I and TAH II
Co2+
-
at 1 mM inhibits by 71.14%
Co2+
39.06% residual activity at 20 mM
Co2+
-
87.6% residual activity at 1 mM
Co2+
25% inhibition at 10 mM
Co2+
-
competitive inhibitor
Co2+
-
inhibits both isozymes TAH I and TAH II
Co2+
-
32% inhibition at 1 mM
Co2+
-
complete inhibition at 1 mM
CO32-
-
inhibits both isozymes TAH I and TAH II
Cu2+
-
at 1 mM inhibits by 51.21%
Cu2+
10.58% residual activity at 20 mM
Cu2+
-
ca. 20% inhibition after 60 min of incubation at 30°C and pH 5
Cu2+
-
41% inhibition of tannase produced under solid-state fermentation at 1 mM
Cu2+
-
mild inhibitory effect
Cu2+
53% inhibition at 10 mM
Cu2+
51% inhibition, at 30°C in sodium citrate buffer, pH 6.0
Cu2+
-
competitive inhibitor
Cu2+
-
inhibits both isozymes TAH I and TAH II
cyanamide
-
-
cyanamide
-
61.7% residual activity at 1 mM
diisopropylfluorophosphate
-
DFP, inhibition is not immediate, but requires a period of preincubation of the enzyme. 1 mol of 32P of DFP is incorporated into 1 mol of enzyme to give complete inhibition, suggest that the enzyme contains one essential serine per mol enzyme, a typical serine enzyme
diisopropylfluorophosphate
-
83% inhibition
dithiothreitol
displays inhibitory properties at higher concentrations of 1.0% to 5.0% (v/v)
dithiothreitol
-
54.1% residual activity at 1 mM
DMSO
-
most potent inhibitor
DMSO
-
1 mM, 19% remaining activity
DMSO
inhibits by 35% at 20%
EDTA
-
complete inhibition at 5 mM
EDTA
-
5 mM EDTA is completely inhibitory for tannase activity
EDTA
-
35% inhibition at 1 mM
EDTA
-
25.89% residual activity at 20 mM
EDTA
-
at 0.01% of concentration 22% inhibition after 5 min and at 0.1% of concentration 19% inhibition after 60 min at 30°C
EDTA
-
activity is completely lost, when the enzyme is dialyzed against 0.025 M EDTA
EDTA
-
95% remaining activity after 3 days at a concentration of 10 mM, pH 7.2, 0.1 M phosphate buffer
EDTA
-
the enzyme is partially inhibited by but not significantly affected (87.8% residual activity at 1 mM)
EDTA
-
about 30% residual activity at 1 mM
EDTA
the enzyme is partially inhibited by but not significantly affected (97.6% residual activity at 1 mM)
EDTA
-
38% inhibition at 1 mM
EDTA
-
1 mM, 30% remaining activity
EDTA
15% inhibition at 1%
EDTA
-
80% residual activity at 1 mM
EDTA
86% residual activity at 1 mM
ethanol
-
inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60%
ethanol
-
80.2% residual activity at 5% (v/v)
ethanol
-
at 60% of concentration, completely inhibits activity at 30°C
ethyl gallate
-
20% inhibition at 1 mM
ethyl gallate
-
1 mM inhibits activity by 48%
Fe2+
-
-
Fe2+
-
63% inhibition of tannase produced under submerged fermentation at 1 mM
Fe2+
55% inhibition at 10 mM
Fe2+
-
24.1% residual activity at 1 mM
Fe2+
-
23.4% residual activity at 1 mM
Fe2+
22.5% residual activity at 1 mM
Fe3+
-
at 1 mM inhibits by 76.89%
Fe3+
-
85.74% residual activity at 1 mM
Fe3+
-
more than 40% inhibition after 5 min and 60 min of incubation at 30°C and pH 5
Fe3+
-
highly inhibited by Fe3+
Fe3+
35% inhibition, at 30°C in sodium citrate buffer, pH 6.0
Fe3+
-
competitive inhibitor
Fe3+
-
inhibits both isozymes TAH I and TAH II
Fe3+
-
complete inhibition at 1 mM
formaldehyde
-
51.5% inhibition at 20% after 60 min at 30°C
formaldehyde
-
complete inactivation
gallic acid
-
82.1% residual activity at 1 mM
gallic acid
-
activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction
gallic acid
-
competitive; i.e. 3,4,5-trihydroxybenzoic acid
gallic acid
competitive inhibition using gallic acid as substrate
heptane
-
at 60% of concentration 95% inhibition after 60 min at 30°C
Hg2+
53.02% residual activity at 20 mM
Hg2+
-
56% residual activity at 5 mM
Hg2+
73% inhibition at 10 mM
Hg2+
-
inhibits both isozymes TAH I and TAH II
Hg2+
-
57% inhibition at 1 mM
Hg2+
-
66% inhibition at 1 mM
Hg2+
-
1 mM inhibits activity by 78%
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
competitive inhibition at 1 mM, 13.8% remaining activity
Hg2+
-
6% residual activity at 1 mM
Hg2+
complete inhibition at 1 mM
Hg2+
-
complete inhibition of the free enzyme at 1 mM, the immobilized enzyme shows 93.7% residual activity at 1 mM
iodoacetic acid
-
-
iodoacetic acid
-
48% residual activity at 1 mM
Isopropanol
-
at 1% v/v
Isopropanol
-
96.3% residual activity at 5% (v/v)
K+
-
73.3% residual activity at 5 mM
K+
-
inhibits both isozymes TAH I and TAH II
K+
-
58% inhibition at 1 mM
K+
-
69% residual activity at 1 mM
methanol
-
initial inhibitory effect at 20% and 40%, original activity is regained at 60%
methanol
-
95.8% residual activity at 5% (v/v)
methanol
inhibits by 56% at 20%
methyl gallate
-
-
Mg2+
7.03% residual activity at 20 mM
Mg2+
-
slight reduction in activity in the presence of Mg2+
Mg2+
-
77.5% residual activity at 5 mM
Mg2+
-
33% inhibition at 20 mM, noncompetitive
Mg2+
-
ca. 20% inhibition after 5 min of incubation at 30°C and pH 5
Mg2+
-
10% inhibition at 1 mM
Mg2+
-
96.7% residual activity at 1 mM
Mg2+
-
16% inhibition at 1 mM
Mg2+
-
1 mM inhibits activity by 12%
Mg2+
-
65% residual activity at 1 mM
Mn2+
10.18% residual activity at 20 mM
Mn2+
-
79% residual activity at 1 mM
Mn2+
-
54% inhibition at 20 mM, noncompetitive
Mn2+
-
ca. 20% inhibition after 5 min of incubation at 30°C and pH 5
Mn2+
-
inhibits both isozymes TAH I and TAH II
Mn2+
-
60% inhibition at 1 mM
Mn2+
-
75.6% residual activity at 1 mM
Mn2+
87.6% residual activity at 1 mM
Mn2+
90% residual activity at 1 mM
N-bromosuccinimide
-
73.9% residual activity at 1 mM
N-bromosuccinimide
-
29% inhibition at 1 mM
N-ethylmaleimide
-
7% residual activity at 1 mM
Na+
-
89.1% residual activity at 5 mM
Na+
-
30% inhibition at 1 mM
Na+
activates by 10% at 1 mM, and inhibits at 5-10 mM
NaCl
-
inhibits above 3 M
NaCl
the enzyme retains 80% activity at 5 M
o-phenanthroline
-
-
Pb2+
-
inhibits both isozymes TAH I and TAH II
phenyl boronic acid
-
-
phenyl boronic acid
-
67.1% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
86.1% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
38% inhibition after 5 min of incubation at 30°C and pH 5, indicating the presence of a serine or cysteine residue in the catalytic site
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
-
the enzyme is partially inhibited by but not significantly affected (66.2% residual activity at 1 mM)
phenylmethylsulfonyl fluoride
-
81.2% residual activity at 1 mM
phenylmethylsulfonyl fluoride
the enzyme is partially inhibited by but not significantly affected (83.2% residual activity at 1 mM)
phenylmethylsulfonyl fluoride
-
28% residual activity at 1 mM
phenylmethylsulfonyl fluoride
-
-
phenylmethylsulfonyl fluoride
32% residual activity at 1 mM
PMSF
-
-
propyl gallate
-
85.1% residual activity at 1 mM
pyrogallol
-
95% residual activity at 1 mM
pyrogallol
-
competitive; i.e. pyrogallol
SDS
-
2% (w/v) SDS is completely inhibitory for tannase activity
SDS
-
at 0.01% of concentration 15% inhibition after 60 min at 30°C
SDS
-
complete inactivation
Sn2+
-
inhibits both isozymes TAH I and TAH II
Sodium azide
-
82.8% residual activity at 1 mM
Sodium azide
-
36% inhibition at 1 mM
Sodium bisulfite
-
81.3% residual activity at 1 mM
Sodium bisulfite
-
25% inhibition at 1 mM
sodium cholate
-
-
sodium cholate
-
52.6% residual activity at 1 mM
sodium lauryl sulfate
-
above 2%
sodium lauryl sulfate
-
-
Sodium thioglycolate
-
-
Sodium thioglycolate
-
83.1% residual activity at 1 mM
tetrahydrofuran
-
at 60% of concentration, completely inhibits activity at 30°C
tetrahydrofuran
-
complete inactivation
Toluene
-
gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49%
Triton X-100
-
-
Triton X-100
-
21.9% inhibition at 1% (v/v)
Triton X-100
-
inhibits both isozymes TAH I and TAH II
Triton X-100
-
32% inhibition at 1 mM
Triton X-100
-
48% inhibition at 1% v/v
Triton X-100
-
78% residual activity at 1 mM
Tween 20
-
30.8% inhibition at 1% (v/v)
Tween 20
-
inhibits both isozymes TAH I and TAH II
Tween 20
-
30% inhibition at 1% v/v
Tween 60
-
-
Tween 60
-
inhibits both isozymes TAH I and TAH II
Tween 60
-
complete inhibition at 1% v/v
Tween 80
-
27.4% inhibition at 1% (v/v)
Tween 80
-
at 0.01% of concentration 35% inhibition after 5 min at 30°C
Tween 80
-
inhibits both isozymes TAH I and TAH II
Tween 80
-
30% inhibition at 1% v/v
Tween 80
47% residual activity at 1 mM
Urea
-
above 3 M
Urea
-
urea concentrations higher than 3 M are completely inhibitory for tannase activity
Urea
-
slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M
Urea
-
the enzyme is partially inhibited by but not significantly affected (96.9% residual activity at 1 mM)
Urea
-
1 mM inhibits activity by 52%
Urea
the enzyme is partially inhibited by but not significantly affected (91.4% residual activity at 1 mM)
Urea
-
at 1.5 M maximal enhancement of activity, at 3 M 50% inhibition
Urea
-
slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M
Urea
-
35% residual activity at 1 mM
Zn2+
30.54% residual activity at 20 mM
Zn2+
-
slight reduction in activity in the presence of Zn2+
Zn2+
-
80% residual activity at 5 mM
Zn2+
-
41% inhibition at 20 mM, noncompetitive
Zn2+
-
ca. 10% inhibition after 5 min and 60 min of incubation at 30°C and pH 5
Zn2+
-
mild inhibitory effect
Zn2+
11% inhibition at 10 mM
Zn2+
-
competitive inhibitor
Zn2+
-
inhibits both isozymes TAH I and TAH II
Zn2+
-
22% inhibition at 1 mM
Zn2+
-
95.4% residual activity at 1 mM
Zn2+
-
25.2% residual activity at 1 mM
Zn2+
-
1 mM inhibits activity by 18%
Zn2+
-
about 65% residual activity at 1 mM
Zn2+
46.1% residual activity at 1 mM
Zn2+
-
competitive inhibition at 1 mM, 21.3% remaining activity
Zn2+
-
10% residual activity at 1 mM
additional information
-
no inhibition of the extracellular enzyme by PMSF
-
additional information
-
1 mM Zn2+ has no effect
-
additional information
-
no or poor inhibition by Ca2+, CuCl2, Hg2+, Mg2+, and Zn2+
-
additional information
-
not inhibited by iodoacetamide
-
additional information
-
not affected by EDTA
-
additional information
-
induction and repression patterns, overview
-
additional information
-
at 0.1% and 0.01% of concentration, Ca2+, Tween 20 and Triton X-100 have no effect at 30°C
-
additional information
-
not inhibited by o-phenanthroline, PMSF, EDTA, 2-mercaptomethanol, sodium thioglycolate
-
additional information
-
not inhibited by benzoic acid and 3-methyl-benzoic acid
-
additional information
-
the enzyme is not affected by EDTA
-
additional information
EDTA, Mg2+, Mn2+, Ca2+, Zn2+ and EDTA have no or only weak effects
-
additional information
-
EDTA, Mg2+, Mn2+, Ca2+, Zn2+ and EDTA have no or only weak effects
-
additional information
-
not inhibited by EDTA
-
additional information
-
no effect at 1 mM by K+, Ca2+, Zn2+, Tween 80, urea, DMSO, and EDTA
-
additional information
-
1 mM DMSO has no significant effect
-
additional information
-
no inhibition at 1 mM iodoacetamide
-
additional information
-
-
-
additional information
non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity
-
additional information
-
non-polar organic solvents increase the tannase activity and polar solvents inhibit the tannase activity
-
additional information
-
no inhibition by Triton X-100
-
additional information
the enzyme is not inhibited by 1-4 M NaCl
-
3.1.1.20 tannase
results (
results (
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