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Literature summary for 3.1.1.20 extracted from
- Synthesis of propyl gallate by mycelium-bound tannase from Aspergillus niger in organic solvent (2007), World J. Microbiol. Biotechnol., 23, 1091-1098.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme | Aspergillus niger |  |
| acetone | - |
Aspergillus niger | |
| Benzene | optimum enzyme concentration should be 0.1 g mycelial dry weight per 10 ml benzene | Aspergillus niger | |
| gallic acid | activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction | Aspergillus niger | |
| hexane | - |
Aspergillus niger | |
| additional information | poor activating effects by octane and dodecane, tannic acid induces the enzyme, effects of organic solvents on propyl gallate production by the enzyme, and effect of water content, overview | Aspergillus niger | |
| N,N-Dimethylformamide | - |
Aspergillus niger | |
| Pyridine | - |
Aspergillus niger | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the mycelium-bound enzyme is useful as biocatalyst in a whole cell system at proper conditions to higher conversion of propyl gallate, the method could also reduce the cost and the time for the immobilization process | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | activates at a concentration of 3.6-7.3% v/v, at higher concentration it inhibits the propyl gallate synthesis reaction causing disruption of essential membrane functions and denaturation of enzyme | Aspergillus niger | |
| gallic acid | activates at a concentration up to 0.005 mM, at higher concentration it inhibits the propyl gallate synthesis reaction | Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tannic acid + H2O | Aspergillus niger | - |
gallic acid + ? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | effects of organic solvents on propyl gallate production by the enzyme, overview | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
inducible enzyme | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | mycelium-bound tannase | Aspergillus niger | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-propanol + gallic acid | the reaction takes place in organic solvents, best in benzene, effect of water content, overview | Aspergillus niger | propyl gallate + H2O | - |
r | |
| tannic acid + H2O | - |
Aspergillus niger | gallic acid + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tannin acyl hydrolase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | - |
Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
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