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3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Word Map on EC 3.1.1.20

Reaction

digallate
+
H2O
= 2 gallate

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.20 tannase

Storage Stability

Storage Stability on EC 3.1.1.20 - tannase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 3 months
-
4°C and 30°C, tannase in both crude and crude lyophilized forms, one year, retains more than 60% residual activity
-
4°C, free enzyme in 100 mM phosphate buffer (pH 6.5), 16 days, complete loss of activity
-
4°C, immobilized enzyme in 100 mM phosphate buffer (pH 6.5), 30 days, less than 10% loss of activity
-
4°C, in 10 mM acetate buffer (pH 5.5), 3 months, no loss of activity
-
5°C, citrate buffer, pH 5.5 or distilled water, as purified powder or as a solution, maintains activity over 6 months
-
shelf-life of crude and crude lyophilized tannase at 4°C and at 30°C, 70% remaining activity after 365 days, overview
-
the stability assays follow zero order kinetics with a reaction rate of 0.01 and 0.48%/h at 4 and 30°C, respectively, decreasing to 98.9 and 44.0 % after 120 h