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3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Word Map on EC 3.1.1.20

Reaction

digallate
+
H2O
= 2 gallate

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.20 tannase

Specific Activity

Specific Activity on EC 3.1.1.20 - tannase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.031
-
crude extract, pH 5.0, 45°C
0.084
crude extract, pH and temperature not specified in the publication
0.1602
-
6 step purified enzyme
0.18
-
purified enzyme, substrate catechin gallate
0.31
-
crude extract, at pH 5.5, 37°C
0.43
-
purified enzyme, substrate epicatechin gallate
0.575
-
crude extract, pH 5.5, 50°C
0.64
-
purified enzyme, substrate epigallocatechin gallate
0.84
-
purified enzyme, substrate tannic acid
0.87
-
purified enzyme, substrate gallic acid methyl ester
1.13
-
purified enzyme, substrate gallocatechin gallate
1.6
-
pH 6.0
1.91
-
after 61.61fold purification, pH 5.0, 45°C
1026.12
-
purified native enzyme, pH 5.5, 30°C
118.8
-
3 step purification
13.63
after 162fold purification, pH and temperature not specified in the publication
135
-
5 step purification
14.65
-
immobilized enzyme
15.27
-
crude filtrate, in 0.5 M citrate phosphate buffer, pH 5.0, at 50°C
170
-
purified enzyme
2.7
Hyalopus sp.
-
fermented broth, in 0.2 M acetate buffer, pH 5.5, at 40°C, for 30 min
20
-
purified enzyme, in 100 mM sodium acetate, pH 5.0, 30% (w/v) diglyme, at 4°C
2067
-
enzyme produced under submerged fermentation, after 477fold purification, at pH 7.0, 30°C
22.85
-
after 39.74fold purification, pH 5.5, 50°C
2453.9
purified enzyme, pH 5.0, 37°C, substrate tannic acid
250
-
commercial preparation
264.5
-
enzyme produced under solid-state fermentation, after 153fold purification, at pH 7.0, 30°C
2762
after 6.32fold purification, pH 2.0, 30°C
33.7
-
crude extract
355.6
-
purified enzyme
40.7
purified recombinant extracellular enzyme, pH 7.0, 30°C
436.7
crude extract, pH 2.0, 30°C
489
-
crude extract
49330
-
pH 5.0, 30°C
5.6
-
purified native enzyme
50
purified recombinant enzyme, pH 5.0, 30°C
6.3
Hyalopus sp.
-
2.33fold purified enzyme, in 0.2 M acetate buffer, pH 5.5, at 40°C, for 30 min
6.49
-
after 21fold purification, at pH 5.5, 37°C
6.8
-
methyl gallate, activity increases 17fold, when the cells are grown in the presence of methyl gallate and 36fold in the presence of tannic acid
61.8
-
purified isozyme TAH I
7.5
crude extract, at pH 8.0
716
recombinant His6-tagged enzyme, pH 7.0, 37°C
72.2
-
free enzyme
78
-
purified enzyme
82.2
-
purified isozyme TAH II
84.34
after 11.25fold purification, at pH 8.0
86
-
3 step purified enzyme
88.3
-
after 2.62fold purification
92
-
8 step purified enzyme
9550
-
19.5fold purified enzyme
additional information