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3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Word Map on EC 3.1.1.20

Reaction

digallate
+
H2O
= 2 gallate

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.20 tannase

General Stability

General Stability on EC 3.1.1.20 - tannase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
a mixture of tannic acid and PEG 6000 protect the enzyme during purification
-
salt-tolerant enzyme, stable up to 2 M NaCl, and 82% remaining activity in presence of 3 M NaCl
-
the enzyme is salt tolerant up to 3 M NaCl
-
the enzyme is salt tolerant, stable up to 2 M of NaCl and retains 82% original activity in 3 M
-
the glyoxyl agarose gel-immobilized enzyme is stabilized 500-1000fold with regard to one-point covalent immobilized derivatives
-
the immobilisation of tannase by multipoint covalent attachment on glyoxyl agarose strongly increases the stability of the enzyme in the presence of 1-propanol (e.g. 10fold more stable than a tannase derivative obtained by a very mild immobilisation on CNBr-activated agarose)
-
the immobilized enzyme retains its initial activity over 10 cycles and retains 80% of its initial activity after 26 cycles. After that, the activity of the immobilized enzyme is quickly lost, retaining about 40% of its initial activity after 46 cycles
-
the tannase can successfully be immobilized on Amberlite IR where it retains about 85% of the initial catalytic activity even after the ninth cycle of its use
-