Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 103.7% activity at 1 mM | Lactiplantibacillus pentosus | |
Urea | 119.5% activity at 1 mM | Lactiplantibacillus pentosus |
Cloned (Comment) | Organism |
---|---|
expressed in in Bacillus subtilis strain RIK 1285 | Lactiplantibacillus pentosus |
expressed in in Bacillus subtilis strain RIK 1285 | Lactiplantibacillus plantarum |
expressed in in Bacillus subtilis strain RIK 1285 | Lactiplantibacillus paraplantarum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 88.7% residual activity at 1 mM | Lactiplantibacillus paraplantarum | |
Ca2+ | 92.3% residual activity at 1 mM | Lactiplantibacillus pentosus | |
Ca2+ | 98.3% residual activity at 1 mM | Lactiplantibacillus plantarum | |
EDTA | the enzyme is partially inhibited by but not significantly affected (87.8% residual activity at 1 mM) | Lactiplantibacillus paraplantarum | |
EDTA | the enzyme is partially inhibited by but not significantly affected (97.6% residual activity at 1 mM) | Lactiplantibacillus plantarum | |
Fe2+ | 24.1% residual activity at 1 mM | Lactiplantibacillus paraplantarum | |
Fe2+ | 23.4% residual activity at 1 mM | Lactiplantibacillus pentosus | |
Fe2+ | 22.5% residual activity at 1 mM | Lactiplantibacillus plantarum | |
Mg2+ | 96.7% residual activity at 1 mM | Lactiplantibacillus pentosus | |
Mn2+ | 75.6% residual activity at 1 mM | Lactiplantibacillus pentosus | |
Mn2+ | 87.6% residual activity at 1 mM | Lactiplantibacillus plantarum | |
phenylmethylsulfonyl fluoride | the enzyme is partially inhibited by but not significantly affected (66.2% residual activity at 1 mM) | Lactiplantibacillus paraplantarum | |
phenylmethylsulfonyl fluoride | 81.2% residual activity at 1 mM | Lactiplantibacillus pentosus | |
phenylmethylsulfonyl fluoride | the enzyme is partially inhibited by but not significantly affected (83.2% residual activity at 1 mM) | Lactiplantibacillus plantarum | |
Urea | the enzyme is partially inhibited by but not significantly affected (96.9% residual activity at 1 mM) | Lactiplantibacillus paraplantarum | |
Urea | the enzyme is partially inhibited by but not significantly affected (91.4% residual activity at 1 mM) | Lactiplantibacillus plantarum | |
Zn2+ | 95.4% residual activity at 1 mM | Lactiplantibacillus paraplantarum | |
Zn2+ | 25.2% residual activity at 1 mM | Lactiplantibacillus pentosus | |
Zn2+ | 46.1% residual activity at 1 mM | Lactiplantibacillus plantarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.03 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.03 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.04 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.04 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.05 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.05 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.05 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.06 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.06 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.06 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.06 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.07 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.08 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.1 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.37 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.5 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.87 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 110.5% activity at 1 mM | Lactiplantibacillus paraplantarum | |
Mg2+ | 123.7% activity at 1 mM | Lactiplantibacillus plantarum | |
Mn2+ | 111.3% activity at 1 mM | Lactiplantibacillus paraplantarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Lactiplantibacillus pentosus |
50000 | - |
x * 50000, SDS-PAGE | Lactiplantibacillus plantarum |
50000 | - |
x * 50000, SDS-PAGE | Lactiplantibacillus paraplantarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | Lactiplantibacillus pentosus | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus plantarum | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus paraplantarum | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus pentosus 21A-3 | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus paraplantarum NSO120 | - |
gallate + D-glucose | - |
? | |
tannic acid + H2O | Lactiplantibacillus plantarum ATCC 14917T | - |
gallate + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus paraplantarum | - |
- |
- |
Lactiplantibacillus paraplantarum NSO120 | - |
- |
- |
Lactiplantibacillus pentosus | - |
- |
- |
Lactiplantibacillus pentosus 21A-3 | - |
- |
- |
Lactiplantibacillus plantarum | B3Y018 | - |
- |
Lactiplantibacillus plantarum ATCC 14917T | B3Y018 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Lactiplantibacillus pentosus |
TALON metal affinity resin column chromatography | Lactiplantibacillus plantarum |
TALON metal affinity resin column chromatography | Lactiplantibacillus paraplantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechin gallate + H2O | - |
Lactiplantibacillus pentosus | ? | - |
? | |
catechin gallate + H2O | - |
Lactiplantibacillus plantarum | ? | - |
? | |
catechin gallate + H2O | - |
Lactiplantibacillus paraplantarum | ? | - |
? | |
catechin gallate + H2O | - |
Lactiplantibacillus pentosus 21A-3 | ? | - |
? | |
catechin gallate + H2O | - |
Lactiplantibacillus paraplantarum NSO120 | ? | - |
? | |
catechin gallate + H2O | - |
Lactiplantibacillus plantarum ATCC 14917T | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus pentosus | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus plantarum | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus paraplantarum | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus pentosus 21A-3 | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus paraplantarum NSO120 | ? | - |
? | |
epicatechin gallate + H2O | - |
Lactiplantibacillus plantarum ATCC 14917T | ? | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus pentosus | gallate + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus paraplantarum | gallate + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus pentosus 21A-3 | gallate + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus paraplantarum NSO120 | gallate + epigallocatechin | - |
? | |
epigallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum ATCC 14917T | gallate + epigallocatechin | - |
? | |
epigallocatechin-3-O-(3-O-methyl) gallate + H2O | - |
Lactiplantibacillus pentosus | ? | - |
? | |
epigallocatechin-3-O-(3-O-methyl) gallate + H2O | - |
Lactiplantibacillus plantarum | ? | - |
? | |
epigallocatechin-3-O-(3-O-methyl) gallate + H2O | - |
Lactiplantibacillus paraplantarum | ? | - |
? | |
gallocatechin gallate + H2O | - |
Lactiplantibacillus pentosus | ? | - |
? | |
gallocatechin gallate + H2O | - |
Lactiplantibacillus plantarum | ? | - |
? | |
gallocatechin gallate + H2O | - |
Lactiplantibacillus paraplantarum | ? | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus pentosus | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus plantarum | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus paraplantarum | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus pentosus 21A-3 | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus paraplantarum NSO120 | gallate + methanol | - |
? | |
methyl gallate + H2O | - |
Lactiplantibacillus plantarum ATCC 14917T | gallate + methanol | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus pentosus | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus paraplantarum | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus pentosus 21A-3 | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus paraplantarum NSO120 | gallate + D-glucose | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum ATCC 14917T | gallate + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Lactiplantibacillus pentosus |
? | x * 50000, SDS-PAGE | Lactiplantibacillus plantarum |
? | x * 50000, SDS-PAGE | Lactiplantibacillus paraplantarum |
Synonyms | Comment | Organism |
---|---|---|
tannin acyl hydrolase | - |
Lactiplantibacillus pentosus |
tannin acyl hydrolase | - |
Lactiplantibacillus plantarum |
tannin acyl hydrolase | - |
Lactiplantibacillus paraplantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactiplantibacillus pentosus |
40 | - |
- |
Lactiplantibacillus plantarum |
40 | - |
- |
Lactiplantibacillus paraplantarum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.26 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
0.35 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
0.42 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.44 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
0.89 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
1.12 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
1.12 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
1.48 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
1.49 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
2.41 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
8.1 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
9.2 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
11.08 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
14.29 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
15.95 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
46.02 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
72.73 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Lactiplantibacillus pentosus |
8.5 | - |
- |
Lactiplantibacillus plantarum |
8.5 | - |
- |
Lactiplantibacillus paraplantarum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 | Lactiplantibacillus pentosus |
8 | 10 | the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 | Lactiplantibacillus plantarum |
8 | 10 | the enzyme shows more than 80% of activities at a pH range of 8.0-10.0 | Lactiplantibacillus paraplantarum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.04 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
7.25 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
8.63 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
9.02 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
10.57 | - |
epigallocatechin-3-O-(3-O-methyl) gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
11.68 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
14.32 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
18.79 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
19.22 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus pentosus | |
27.19 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
52.23 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
53.65 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
125 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus plantarum | |
145.1 | - |
methyl gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
154.7 | - |
gallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
181.5 | - |
catechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
195.3 | - |
epicatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum | |
260.8 | - |
epigallocatechin gallate | in 50 mM Tris-HCl (pH 8.0), at 37°C | Lactiplantibacillus paraplantarum |