Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Word Map on EC 3.1.1.20

Reaction

digallate
+
H2O
= 2 gallate

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.20 tannase

Cloned

Cloned on EC 3.1.1.20 - tannase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1.4 kb purified PCR product of tanLp1 gene cloned into pURI3 vector and transformed into Escherichia coli DH5alpha cells. Recombinant plasmids isolated and overexpressed in Escherichia coli JM109 (DE3)
-
cloned in Escherichia coli JM 109 and expressed in Aspergillus oryzae strain AO1, a tannase low-producing and nitrate reductase-deficient strain
-
DNA and amino acid sequence determination and analysis, expressionin Escherichia coli strain DH5alpha
DNA and amino acid sequence determination nand analysis, sequence comparisons and phylogenetic tree, recombinant expression in Pichia pastoris strain KM71 of the tannase, without the signal sequence, but with secretion signal sequence of Saccharomyces cerevisiae instead of the original signal sequence, the enzyme is secreted
expressed in Arxula adeninivorans strain G1212
-
expressed in Escherichia coli BL21(DE3) and B834(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-AI cells
expressed in Escherichia coli DH5alpha
expressed in in Bacillus subtilis strain RIK 1285
expressed in Pichia pastoris strain GS115
expressed in Pichia pastoris strain RIB40
-
gene AotanB, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, without the signal sequence, but with secretion signal sequence of Saccharomyces cerevisiae instead of the original signal sequence, recombinant expression in Pichia pastoris strain GS115 from vector pPICZalphaA, the enzyme is secreted into the medium. Recombinant AoTanB is highly expressed by incubation at 18°C compared to 30°C
gene tan BLP, DNA and amino acid sequence determination and analysis
-
gene tan7, recombinant expression in Aspergillus niger strain Bdel4, the recombinant enzyme is secreted to the culture medium
gene tanA, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene tanA, recombinant expression of wild-type and mutant enzymes in Pichia pastoris strain GS115, recombinant AoTanA and its mutant variants are secreted using a Saccharomyces cerevisiae alpha-factor secretion signal peptide fusion, the recombinant proteins accumulate in the culture broth
gene tanLpl, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant ATAN1 gene overexpressed in the auxotrophic mutant strain Arxula adeninivorans G1212 [DELTAatrp1] under the control of the strong, constitutive TEF1 promoter. Expression module assembled in the plasmid pGH101-TEF1-ATAN1 and used to construct YIEC101-TEF1-ATAN1 for transformation of Arxula host cells
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)