Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzene | increases activity by 2fold at 60% v/v concentration | Aspergillus awamori | |
butanol | increases activity by 2fold at 60% v/v concentration | Aspergillus awamori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetic acid | completely inhibits activity | Aspergillus awamori | |
acetone | gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 55.01% | Aspergillus awamori | |
chloroform | completely inhibits activity | Aspergillus awamori | |
Co2+ | at 1 mM inhibits by 71.14% | Aspergillus awamori | |
Cu2+ | at 1 mM inhibits by 51.21% | Aspergillus awamori | |
ethanol | inhibits by 48.84% initially, thereafter complete loss in the enzyme activity at 40% and 60% | Aspergillus awamori | |
Fe3+ | at 1 mM inhibits by 76.89% | Aspergillus awamori | |
Isoamylalcohol | completely inhibits activity | Aspergillus awamori | |
Isopropyl alcohol | completely inhibits activity | Aspergillus awamori | |
methanol | initial inhibitory effect at 20% and 40%, original activity is regained at 60% | Aspergillus awamori | |
additional information | 1 mM Zn2+ has no effect | Aspergillus awamori | |
Toluene | gradual decrease in activity with increasing concentration, at 60%, activity is reduced to 28.49% | Aspergillus awamori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 1 mM stimulates by 16% | Aspergillus awamori | |
K+ | at 1 mM stimulates by 9% | Aspergillus awamori | |
Mg2+ | at 1 mM stimulates by 23.9% | Aspergillus awamori | |
Mn2+ | at 1 mM stimulates by 15.23% | Aspergillus awamori | |
Na+ | at 1 mM stimulates by 11% | Aspergillus awamori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
x * 101000, SDS-PAGE | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
MTCC9299 | - |
Aspergillus awamori MTCC9299 | - |
MTCC9299 | - |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation followed by ion-exchange chromatography, 19.5folf with 13.5% yield, to homogeneity | Aspergillus awamori |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
489 | - |
crude extract | Aspergillus awamori |
9550 | - |
19.5fold purified enzyme | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tannic acid + H2O | - |
Aspergillus awamori | gallic acid + D-glucose | - |
? | |
tannic acid + H2O | - |
Aspergillus awamori MTCC9299 | gallic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 101000, SDS-PAGE | Aspergillus awamori |
Synonyms | Comment | Organism |
---|---|---|
tannase | - |
Aspergillus awamori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Aspergillus awamori |