3.1.1.20: tannase

This is an abbreviated version!
For detailed information about tannase, go to the full flat file.

Reaction

Synonyms

An04g04430, AoTanA, AotanB, ATAN1, depsidase, fungal tannase, gallotannin-degrading esterase, GALLO_1609, LP-tan, plant tannase, TAH, TAH I, TAH II, tan A, Tan410, tan7, TanA, TanB, tanBLP, TanLpl, tannase, tannase I, tannase II, tannin acyl hydrolase, tannin acyl-hydrolase, tannin acylhydrolase, tannin-acyl-hydrolase, TanSg1, yeast tannase

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.20 tannase

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Results	in table
83	Activating Compound
49	AA Sequence
107	Application
1	CAS Registry Number
27	Cloned(Commentary)
4	Crystallization (Commentary)
35	Expression
24	General Information
8	General Stability
499	Inhibitors
69	kcat/KM [mM/s]
7	Ki Value [mM]
159	KM Value [mM]
55	Localization
141	Metals/Ions
99	Molecular Weight [Da]
269	Natural Substrates/ Products (Substrates)
73	Organic Solvent Stability
333	Organism
1	Oxidation Stability
2	PDB ID
149	pH Optimum
42	pH Range
46	pH Stability
12	pI Value
38	Posttranslational Modification
47	Protein Variants
80	Purification (Commentary)
1	Reaction
27	Reaction Type
289	Reference
62	Source Tissue
69	Specific Activity [micromol/min/mg]
8	Storage Stability
828	Substrates and Products (Substrate)
112	Subunits
239	Synonyms
1	Systematic Name
141	Temperature Optimum [°C]
34	Temperature Range [°C]
97	Temperature Stability [°C]
65	Turnover Number [1/s]

Organic Solvent Stability

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Organic Solvent Stability on EC 3.1.1.20 - tannase

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ORGANIC SOLVENT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

1-propanol

Aspergillus nidulans

-

the free purified enzyme is stable for 96 h with 30% (v/v) and 50% (v/v) 1-propanol at 25°C

729469

2-mercaptomethanol

Talaromyces variabilis

-

39.6% residual activity at 1 mM

691358

Acetone

5 entries

acetonitrile

3 entries

benzene

2 entries

Brij-35

Aspergillus awamori

C7F6Y1

the enzyme is not stable in the presence of Brij-35, with less than 82% residual activity at 0.1-1% (v/v)

715876

Butanol

4 entries

carbon tetrachloride

Talaromyces variabilis

-

57% residual activity after 60 min at 60% (v/v)

691358

chloroform

Aspergillus awamori

C7F6Y1

about 60% activity after 1 h at 10% (v/v)

715876

DMSO

2 entries

Ethanol

8 entries

Ethylene glycol

Aspergillus nidulans

-

in the presence of 50% (v/v) ethylene glycol, the enzyme immobilized on glyoxyl-agarose and aminoglyoxyl-agarose maintains about 75-95% of activity at 75 and 80°C for 3 h, at pH 7.0

729469

formaldehyde

Talaromyces variabilis

-

12% residual activity after 5 min at 20% (v/v)

691358

Glycerol

4 entries

heptane

Talaromyces variabilis

-

62% residual activity after 60 min at 60% (v/v)

691358

hexane

Aspergillus awamori

C7F6Y1

about 80% activity after 1 h at 10% (v/v)

715876

isoamyl alcohol

Bacillus subtilis

-

the activity of tannase increases in presence of isoamyl alcohol

729424

isopropanol

4 entries

Methanol

6 entries

n-Butanol

Aspergillus nidulans

-

140.95% activity at 1% (v/v)

715957

petroleum ether

Talaromyces variabilis

-

33% residual activity after 60 min at 60% (v/v)

691358

SDS

4 entries

tetrahydrofuran

Talaromyces variabilis

-

no activity after 5 min at 20% (v/v)

691358

toluene

4 entries

Triton X-100

3 entries

Tween 60

3 entries

Tween 80

2 entries

additional information

6 entries

Acetone

Aspergillus awamori

C7F6Y1

about 70% activity after 1 h at 10% (v/v)

715876

Acetone

Aspergillus awamori BTMFW032

-

about 70% activity after 1 h at 10% (v/v)

-

Acetone

Aspergillus nidulans

-

130.37% activity at 1% (v/v)

715957

Acetone

Talaromyces variabilis

-

49% residual activity after 60 min at 20% (v/v)

691358

Acetone

Talaromyces variabilis IARI 2031

-

49% residual activity after 60 min at 20% (v/v)

-

acetonitrile

Aspergillus awamori

C7F6Y1

about 85% activity after 1 h at 10% (v/v)

715876

acetonitrile

Aspergillus awamori BTMFW032

-

about 85% activity after 1 h at 10% (v/v)

-

acetonitrile

Aspergillus nidulans

-

149.85% activity at 1% (v/v)

715957

benzene

Aspergillus awamori

C7F6Y1

about 45% activity after 1 h at 10% (v/v)

715876

benzene

Aspergillus heteromorphus

-

116.7% activity at 1% (v/v)

714559

Butanol

Aspergillus awamori

C7F6Y1

about 70% activity after 1 h at 10% (v/v)

715876

Butanol

Aspergillus awamori BTMFW032

-

about 70% activity after 1 h at 10% (v/v)

-

Butanol

Aspergillus heteromorphus

-

115.5% residual activity at 5% (v/v)

714559

Butanol

Aspergillus heteromorphus MTCC 8818

-

115.5% residual activity at 5% (v/v)

-

DMSO

Aspergillus awamori

C7F6Y1

at a 60% concentration of DMSO, the enzyme retains a 61% activity after 24 h

715876

DMSO

Aspergillus heteromorphus

-

122.4% residual activity at 5% (v/v)

714559

Ethanol

Aspergillus awamori

C7F6Y1

about 80% activity after 1 h at 10% (v/v)

715876

Ethanol

Aspergillus awamori BTMFW032

-

about 80% activity after 1 h at 10% (v/v)

-

Ethanol

Aspergillus heteromorphus

-

104.4% residual activity at 1% (v/v)

714559

Ethanol

Aspergillus heteromorphus MTCC 8818

-

104.4% residual activity at 1% (v/v)

-

Ethanol

Aspergillus nidulans

-

148.47% activity at 1% (v/v)

715957

Ethanol

Bacillus subtilis

-

the activity of tannase increases in presence of ethanol

729424

Ethanol

Talaromyces variabilis

-

35.7% residual activity after 60 min at 60% (v/v)

691358

Ethanol

Talaromyces variabilis IARI 2031

-

35.7% residual activity after 60 min at 60% (v/v)

-

Glycerol

Aspergillus heteromorphus

-

118.2% residual activity at 1% (v/v)

714559

Glycerol

Aspergillus heteromorphus MTCC 8818

-

118.2% residual activity at 1% (v/v)

-

Glycerol

Aspergillus nidulans

-

138.5% activity at 1% (v/v)

715957

Glycerol

Bacillus subtilis

-

the activity of tannase increases in presence of glycerol

729424

isopropanol

Aspergillus awamori

C7F6Y1

about 70% activity after 1 h at 10% (v/v)

715876

isopropanol

Aspergillus heteromorphus

-

106.6% activity at 1% (v/v)

714559

isopropanol

Aspergillus nidulans

-

150% activity at 1% (v/v)

715957

isopropanol

Bacillus subtilis

-

the activity of tannase increases in presence of isopropanol

729424

Methanol

Aspergillus awamori

C7F6Y1

about 80% activity after 1 h at 10% (v/v)

715876

Methanol

Aspergillus awamori BTMFW032

-

about 80% activity after 1 h at 10% (v/v)

-

Methanol

Aspergillus heteromorphus

-

103.2% residual activity at 1% (v/v)

714559

Methanol

Aspergillus heteromorphus MTCC 8818

-

103.2% residual activity at 1% (v/v)

-

Methanol

Aspergillus nidulans

-

139.57% activity at 1% (v/v)

715957

Methanol

Bacillus subtilis

-

the activity of tannase increases in presence of methanol

729424

SDS

Aspergillus foetidus

-

the enzyme shows considerable stability in the presence of SDS

691971

SDS

Aspergillus foetidus MTCC 6322

-

the enzyme shows considerable stability in the presence of SDS

-

SDS

Talaromyces variabilis

-

complete inactivation at 1% (w/v)

691358

SDS

Talaromyces variabilis IARI 2031

-

complete inactivation at 1% (w/v)

-

toluene

Aspergillus heteromorphus

-

119.4% residual activity at 1% (v/v)

714559

toluene

Aspergillus heteromorphus MTCC 8818

-

119.4% residual activity at 1% (v/v)

-

toluene

Talaromyces variabilis

-

44% residual activity after 60 min at 60% (v/v)

691358

toluene

Talaromyces variabilis IARI 2031

-

44% residual activity after 60 min at 60% (v/v)

-

Triton X-100

Aspergillus awamori

C7F6Y1

at high concentrations of between 0.6% (v/v) (79.06% residual activity) to 1.0% (v/v) (54.26% residual activity), lead to a loss of enzyme stability, although the enzyme is stable at a 0.2% (v/v) concentration (112.2% residual activity) with an enhanced enzyme activity at a 0.4% concentration (171.36% residual activity)

715876

Triton X-100

Aspergillus foetidus

-

considerable loss in activity in the presence of Triton X-100

691971

Triton X-100

Aspergillus foetidus MTCC 6322

-

considerable loss in activity in the presence of Triton X-100

-

Tween 60

Aspergillus foetidus

-

considerable loss in activity in the presence of Tween 60

691971

Tween 60

Aspergillus foetidus MTCC 6322

-

considerable loss in activity in the presence of Tween 60

-

Tween 60

Talaromyces variabilis

-

complete inactivation at 1% (v/v)

691358

Tween 80

Aspergillus awamori

C7F6Y1

Tween 80 leads to an enhanced enzyme activity at up to a 0.6% (v/v) concentration (157.89% residual activity), but displays inhibitory properties at a high concentration of 1.0% (v/v) (83.5% residual activity)

715876

Tween 80

Talaromyces variabilis

-

complete inactivation at 1% (v/v)

691358

additional information

Aspergillus foetidus

-

the enzyme shows considerable stability in the presence of commercial detergents such as Sunlight, Rinshakthi and Wheel

691971

additional information

Aspergillus foetidus MTCC 6322

-

the enzyme shows considerable stability in the presence of commercial detergents such as Sunlight, Rinshakthi and Wheel

-

additional information

Aspergillus niger

-

effects of organic solvents on propyl gallate production by the enzyme, overview

682999

additional information

Talaromyces variabilis

-

not affected by Triton X-100

691358

additional information

Talaromyces variabilis IARI 2031

-

not affected by Triton X-100

-

additional information

uncultured bacterium

E7D7J5

the enzyme is not influenced by Triton X-100

715232