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Literature summary for 3.1.1.20 extracted from

  • Liu, F.; Wang, B.; Ye, Y.; Pan, L.
    High level expression and characterization of tannase tan7 using Aspergillus niger SH-2 with low-background endogenous secretory proteins as the host (2018), Protein Expr. Purif., 144, 71-75 .
    View publication on PubMed

Application

Application Comment Organism
food industry tannin acyl hydrolase catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid Aspergillus niger
synthesis tannin acyl hydrolase catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid Aspergillus niger

Cloned(Commentary)

Cloned (Comment) Organism
gene tan7, recombinant expression in Aspergillus niger strain Bdel4, the recombinant enzyme is secreted to the culture medium Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Aspergillus niger
Ca2+
-
Aspergillus niger
Cu2+
-
Aspergillus niger
EDTA
-
Aspergillus niger
Mg2+
-
Aspergillus niger
Ni2+
-
Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates by about 30% Aspergillus niger
Mn2+ activates by about 30% Aspergillus niger
additional information K+, Na+ and Cd2+ do not impact the tannase activity Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger A2QIR3
-
-
Aspergillus niger CBS 513.88 A2QIR3
-
-
Aspergillus niger FGSC A1513 A2QIR3
-
-
Aspergillus niger SH-2 A2QIR3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the recombinant extracellular enzyme is glycosylated, deglycosylation by PNGase F Aspergillus niger

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 1.3fold from Aspergillus niger strain Bdel4 culture medium by desalting gel filtration, and gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40.7
-
purified recombinant extracellular enzyme, pH 7.0, 30°C Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propyl gallate + H2O enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) Aspergillus niger gallic acid + propanol
-
?
propyl gallate + H2O enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) Aspergillus niger SH-2 gallic acid + propanol
-
?
propyl gallate + H2O enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) Aspergillus niger FGSC A1513 gallic acid + propanol
-
?
propyl gallate + H2O enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) Aspergillus niger CBS 513.88 gallic acid + propanol
-
?

Subunits

Subunits Comment Organism
? x * 61700, about, sequence calculation, x * 70000-100000, recombinant glycosylated enzyme, SDS-PAGE, x * 60000-70000, recombinant deglycosylated enzyme, SDS-PAGE Aspergillus niger

Synonyms

Synonyms Comment Organism
An04g04430 locus name Aspergillus niger
tan7
-
Aspergillus niger
tannin acyl hydrolase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant enzyme Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 activity range, tannase activity increases at 20°C through 40°C and displays maximum at 40°C, at temperatures above 40°C, the tannase activity decreases sharply Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
recombinant enzyme Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 puriifed recombinant extracellular enzyme, stable in the pH range from pH 6.0-7.5 Aspergillus niger