Application | Comment | Organism |
---|---|---|
food industry | tannin acyl hydrolase catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid | Aspergillus niger |
synthesis | tannin acyl hydrolase catalyzes the hydrolysis of hydrolyzable tannins. It is used in the manufacture of instant tea and in the production of gallic acid | Aspergillus niger |
Cloned (Comment) | Organism |
---|---|
gene tan7, recombinant expression in Aspergillus niger strain Bdel4, the recombinant enzyme is secreted to the culture medium | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Aspergillus niger | |
Ca2+ | - |
Aspergillus niger | |
Cu2+ | - |
Aspergillus niger | |
EDTA | - |
Aspergillus niger | |
Mg2+ | - |
Aspergillus niger | |
Ni2+ | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates by about 30% | Aspergillus niger | |
Mn2+ | activates by about 30% | Aspergillus niger | |
additional information | K+, Na+ and Cd2+ do not impact the tannase activity | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A2QIR3 | - |
- |
Aspergillus niger CBS 513.88 | A2QIR3 | - |
- |
Aspergillus niger FGSC A1513 | A2QIR3 | - |
- |
Aspergillus niger SH-2 | A2QIR3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant extracellular enzyme is glycosylated, deglycosylation by PNGase F | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme 1.3fold from Aspergillus niger strain Bdel4 culture medium by desalting gel filtration, and gel filtration | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
40.7 | - |
purified recombinant extracellular enzyme, pH 7.0, 30°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propyl gallate + H2O | enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) | Aspergillus niger | gallic acid + propanol | - |
? | |
propyl gallate + H2O | enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) | Aspergillus niger SH-2 | gallic acid + propanol | - |
? | |
propyl gallate + H2O | enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) | Aspergillus niger FGSC A1513 | gallic acid + propanol | - |
? | |
propyl gallate + H2O | enzyme activity detection using a method based on the formation of chromogen between enzyme released gallic acid and rhodanine (2-thio-4-ketothiazolidine) | Aspergillus niger CBS 513.88 | gallic acid + propanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61700, about, sequence calculation, x * 70000-100000, recombinant glycosylated enzyme, SDS-PAGE, x * 60000-70000, recombinant deglycosylated enzyme, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
An04g04430 | locus name | Aspergillus niger |
tan7 | - |
Aspergillus niger |
tannin acyl hydrolase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | activity range, tannase activity increases at 20°C through 40°C and displays maximum at 40°C, at temperatures above 40°C, the tannase activity decreases sharply | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant enzyme | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | puriifed recombinant extracellular enzyme, stable in the pH range from pH 6.0-7.5 | Aspergillus niger |