Cloned (Comment) | Organism |
---|---|
gene tanLpl, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Lactiplantibacillus plantarum |
recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces sviceus |
Protein Variants | Comment | Organism |
---|---|---|
D453A | site-directed mutagenesis | Streptomyces sviceus |
D455A | site-directed mutagenesis | Streptomyces sviceus |
E385A | site-directed mutagenesis | Streptomyces sviceus |
G384P | site-directed mutagenesis, the mutant shows 138.1% esterase activity compared to wild-type, and a lower depsidase activity (61.1%) | Streptomyces sviceus |
H485A | site-directed mutagenesis | Streptomyces sviceus |
K371A | site-directed mutagenesis | Streptomyces sviceus |
additional information | an enzyme variant lacking the flap-like domain shows reduced esterase (68%) and highly increased depsidase (244%) activity compared to the wild-type enzyme | Lactiplantibacillus plantarum |
additional information | an enzyme variant lacking the flap-like domain shows slightly reduced esterase and reduced depsidase activity compared to the wild-type enzyme | Streptomyces sviceus |
P356G | site-directed mutagenesis, the mutant P356G only has 48.2% esterase activity compared to wild-type, while it shows a higher depsidase activity (131.7%) than the wild-type enzyme | Lactiplantibacillus plantarum |
S210A | site-directed mutagenesis | Streptomyces sviceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk kinetics | Lactiplantibacillus plantarum | |
additional information | - |
additional information | Lineweaver-Burk kinetics | Streptomyces sviceus | |
0.21 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus | |
0.41 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant P356G | Streptomyces sviceus | |
0.53 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum | |
0.58 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus | |
0.67 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
0.94 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
1.1 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum | |
1.23 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
1.3 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
2.4 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant G384P | Streptomyces sviceus | |
3.3 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus | |
3.5 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | B3Y018 | - |
- |
Streptomyces sviceus | A0A077YGC3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Lactiplantibacillus plantarum |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Streptomyces sviceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl gallate + H2O | - |
Lactiplantibacillus plantarum | methanol + gallic acid | - |
? | |
methyl gallate + H2O | - |
Streptomyces sviceus | methanol + gallic acid | - |
? | |
additional information | wild-type enzyme LP-tan has a higher esterase activity than depsidase activity | Lactiplantibacillus plantarum | ? | - |
? | |
additional information | wild-type enzyme SS-tan has a higher depsidase activity than esterase activity | Streptomyces sviceus | ? | - |
? | |
tannic acid + H2O | - |
Lactiplantibacillus plantarum | gallic acid + ? | - |
? | |
tannic acid + H2O | - |
Streptomyces sviceus | gallic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, C-terminally His-tagged enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
? | x * 51000, C-terminally His-tagged enzyme, SDS-PAGE | Streptomyces sviceus |
More | the enzyme LP-tan displays alpha/beta structure, featured by a large cap domain inserted into the classical serine hydrolase fold, which is familiar with the feruloyl esterase and lipases. Structure comparisons, overview | Lactiplantibacillus plantarum |
Synonyms | Comment | Organism |
---|---|---|
depsidase | - |
Lactiplantibacillus plantarum |
depsidase | - |
Streptomyces sviceus |
LP-tan | - |
Lactiplantibacillus plantarum |
TanLpl | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactiplantibacillus plantarum |
50 | - |
- |
Streptomyces sviceus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60.7 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus | |
62.6 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
64.7 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus | |
66.9 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
69.2 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum | |
69.9 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus | |
70.3 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant P356G | Streptomyces sviceus | |
79.8 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
85.1 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant G384P | Streptomyces sviceus | |
99.4 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
104.6 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus | |
115.5 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Lactiplantibacillus plantarum |
8 | - |
- |
Streptomyces sviceus |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme LP-tan forms a flap domain (amino acids 225-247) and a sandwich structure (Ile206-substrate-Pro356), functional role of sandwich structure and the flap (flap-like) domain in the substrate specificity of tannase, overview. The sandwich and the flap domain can help in catalytic hydrolysis of ester bonds | Lactiplantibacillus plantarum |
additional information | the enzyme SS-tan forms a sandwich structure (Ile253-substrate-Gly384), but no flap domain, it forms a flap-like domain (amino acids: 93-143) instead, functional role of sandwich structure and the flap (flap-like) domain in the substrate specificity of tannase, overview. The sandwich and the flap domain can help in catalytic hydrolysis of ester bonds, while the flap-like domain in SS-tan mainly works on depside bonds | Streptomyces sviceus |
physiological function | tannases can catalyze the hydrolysis of galloyl ester and depside bonds of hydrolysable tannins to release gallic acid and glucose, but tannases from different species have different substrate specificities. The enzymes can also show depsidase activity | Lactiplantibacillus plantarum |
physiological function | tannases can catalyze the hydrolysis of galloyl ester and depside bonds of hydrolysable tannins to release gallic acid and glucose, but tannases from different species have different substrate specificities. The enzymes can also show depsidase activity | Streptomyces sviceus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17.3 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus | |
21.2 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus | |
29.3 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant P356G | Streptomyces sviceus | |
50.9 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
61.4 | - |
methyl gallate | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
71.2 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant P356G | Lactiplantibacillus plantarum | |
105 | - |
methyl gallate | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum | |
130.6 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain-lacking enzyme mutant | Lactiplantibacillus plantarum | |
148.4 | - |
methyl gallate | pH 7.0, 37°C, recombinant wild-type enzyme | Lactiplantibacillus plantarum | |
180.4 | - |
Tannic acid | pH 7.0, 37°C, recombinant flap domain lacking enzyme mutant | Streptomyces sviceus | |
207.6 | - |
Tannic acid | pH 7.0, 37°C, recombinant mutant G384P | Streptomyces sviceus | |
308.1 | - |
Tannic acid | pH 7.0, 37°C, recombinant wild-type enzyme | Streptomyces sviceus |