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2.7.4.1: ATP-polyphosphate phosphotransferase

This is an abbreviated version!
For detailed information about ATP-polyphosphate phosphotransferase, go to the full flat file.

Word Map on EC 2.7.4.1

Reaction

ATP
+
(Phosphate)n
=
ADP
 +
(phosphate)n+1

Synonyms

AAur2811, AjPPK, ATP:polyphosphate phosphotransferase, AUJ86_09545, CHU0107, CHU_0107, class I PPK2, class II polyphosphate kinase, class III PPK2, Deipr_1912, DR0132, DR_0132, ES2, family-2 polyphosphate kinase, FTT_1564, glycogen-bound polyphosphate kinase, GmSuSy, I601_4057, K649_10090, kinase, polyphosphate (phosphorylating), More, MrH2468, Mrub_2488, MXAN_0056, NCg12620, OsIPK2, PA0141, PA3455 protein, paPpx, plyphosphate kinase 1, poly P kinase 1, poly(P) kinase 1, poly(P) kinase 2, poly-P kinase, poly-P kinase 1, polyP kinase, polyphosphate kinase, polyphosphate kinase 1, polyphosphate kinase 2, polyphosphate kinase 3, polyphosphate kinase-1, polyphosphate kinase-2, polyphosphate-dependent nucleoside diphosphate kinase, polyphosphate: ADP phosphotransferase, polyphosphate:ADP phosphotransferase, polyphosphate:nucleotide phosphotransferase, polyphosphoric acid kinase, PPK, PPK-2, PPK1, PPK2, PPK2-1, PPK2-2, PPK2-3, PPK2a, PPK2B, PPK2c, PPK2d, PPK2e, PPK3, Rv3232c, SMc02148, SMc02148 protein, SPO0224, SPO1256, SPO1727

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.1 ATP-polyphosphate phosphotransferase

Purification

Purification on EC 2.7.4.1 - ATP-polyphosphate phosphotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and Mono-Q column chromatography
-
ammonium sulfate, DE52, hydroxylapatite
-
ammonium sulfate, DEAE-cellulose, Sephadex G-200
ammonium sulfate, phosphocellulose, DEAE-cellulose, Mono S
-
ammonium sulfate, phosphocellulose, DEAE-Sepharose
-
ammonium sulfate, Sephadex G-200
-
heparin-Sepharose, GTP agarose, Mono Q, Mono S
native enzyme 480fold by heparin affinity chromatography and two different steps of anion exchange chromatography
native enzyme as glycogen complex partially from crude extract by isopycnic centrifugation in CsCl, followed by dialysis
-
Ni-NTA column chromatography
-
Ni2+-NTA agarose column chromatography
nickel affinity resin column chromatography
-
nickel-charged His-Trap chelating column chromatography
-
of the overexpessed protein
-
of the recombinant His x6-tagged protein
partial purification
protamine sulfate, alumina gel, acetone
-
purified 1300fold to homogeneity from lysates
recombinant His-tagged polyphosphate kinase, Ni-NTA affinity column
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta pLysS (DE3) by nickel affinity chromatography and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged Ppk 21fold from Escherichia coli strain BL21(DE3) by His-Trap chromatography
Thermosynechococcus vestitus
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus by nickel affinity chromatography, dialysis, tag cleavage through thrombin, and again dialysis
-
recombinant protein, Ni-chelating affinity chromatography
recombinant Strep-tagged isozyme PPK2-1 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography
recombinant Strep-tagged isozyme PPK2-2 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography
recombinant Strep-tagged isozyme PPK2-3 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography
streptomycin sulfate, ammonium sulfate
-