Literature summary for 2.7.4.1 extracted from

Shi, T.; Ge, Y.; Zhao, N.; Hu, X.; Yuan, Z.
Polyphosphate kinase of Lysinibacillus sphaericus and its effects on accumulation of polyphosphate and bacterial growth (2015), Microbiol. Res., 172, 41-47.

Search for more literature for 2.7.4.1

Cloned(Commentary)

Cloned (Comment)	Organism
gene ppk, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha	Lysinibacillus sphaericus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a ppk null mutant and the ppk-complemented strain, DELTAppk and DELTAppk (pHT304-ppk)	Lysinibacillus sphaericus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	complete inhibition	Lysinibacillus sphaericus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (phosphate)n	Lysinibacillus sphaericus	-	ADP + (phosphate)n+1	-	r

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	B1HP60	formerly Bacillus sphaericus , gene ppk	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (phosphate)n	-	Lysinibacillus sphaericus	ADP + (phosphate)n+1	-	r

Synonyms

Synonyms	Comment	Organism
PPK	-	Lysinibacillus sphaericus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Lysinibacillus sphaericus

General Information

General Information	Comment	Organism
malfunction	deletion of gene ppk results in a deficiencyin polyphosphate accumulation rather than cell growth in Luria-Bertani medium. The cell growth is evidently retarded and the ilvBHC expression is significantly reduced when the ppk mutant is transferred from LB to limited-amino acid medium. These phenotypes are significantly restored in the ppk-complemented strain	Lysinibacillus sphaericus
metabolism	PPK is a primary enzyme involved in polyphosphate biosynthesis	Lysinibacillus sphaericus
physiological function	polyphosphate is essential for cell growth and responses to nutritional stringen-cies and environmental stresses and branched-chain amino acids are useful intracellular signals for bacterial adaption to nutritional environments. Enzyme PPK plays a rolein polyphosphate accumulation and expression of genes involved in branched-chain amino acids biosynthesis	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)