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2.7.4.1: ATP-polyphosphate phosphotransferase

This is an abbreviated version!
For detailed information about ATP-polyphosphate phosphotransferase, go to the full flat file.

Word Map on EC 2.7.4.1

Reaction

ATP
+
(Phosphate)n
=
ADP
 +
(phosphate)n+1

Synonyms

AAur2811, AjPPK, ATP:polyphosphate phosphotransferase, AUJ86_09545, CHU0107, CHU_0107, class I PPK2, class II polyphosphate kinase, class III PPK2, Deipr_1912, DR0132, DR_0132, ES2, family-2 polyphosphate kinase, FTT_1564, glycogen-bound polyphosphate kinase, GmSuSy, I601_4057, K649_10090, kinase, polyphosphate (phosphorylating), More, MrH2468, Mrub_2488, MXAN_0056, NCg12620, OsIPK2, PA0141, PA3455 protein, paPpx, plyphosphate kinase 1, poly P kinase 1, poly(P) kinase 1, poly(P) kinase 2, poly-P kinase, poly-P kinase 1, polyP kinase, polyphosphate kinase, polyphosphate kinase 1, polyphosphate kinase 2, polyphosphate kinase 3, polyphosphate kinase-1, polyphosphate kinase-2, polyphosphate-dependent nucleoside diphosphate kinase, polyphosphate: ADP phosphotransferase, polyphosphate:ADP phosphotransferase, polyphosphate:nucleotide phosphotransferase, polyphosphoric acid kinase, PPK, PPK-2, PPK1, PPK2, PPK2-1, PPK2-2, PPK2-3, PPK2a, PPK2B, PPK2c, PPK2d, PPK2e, PPK3, Rv3232c, SMc02148, SMc02148 protein, SPO0224, SPO1256, SPO1727

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.1 ATP-polyphosphate phosphotransferase

Specific Activity

Specific Activity on EC 2.7.4.1 - ATP-polyphosphate phosphotransferase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
Thermosynechococcus vestitus
of the purified protein
0.00067
-
GTP formation from GDP, pH 7.0, 37°C, wild-type clone AG83
0.0012
-
GTP formation from GDP, pH 7.0, 37°C, SSG-resistant clone AG83
0.00208
-
GTP formation from GDP, pH 7.0, 37°C, PMM-resistant clone AG83
0.0055
polyphosphate kinase 2
0.0335
-
ATP formation from ADP, pH 7.0, 37°C, PMM-resistant clone AG83
0.034
recombinant polyphosphate kinase
0.0369
-
ATP formation from ADP, pH 7.0, 37°C, SSG-resistant clone AG83
0.0442
-
ATP formation from ADP, pH 7.0, 37°C, wild-type clone AG83
0.048
purified enzyme
0.13
-
polyphosphate kinase activity in crude extracts measured with a modified toluidine blue assay
0.25
synthesis of ATP, pH 8, 37°C
0.317
Thermosynechococcus vestitus
purified recombinant His-tagged Ppk
0.7
-
synthesis of ATP, reversed reaction
1.8
substrate ADP, pH 7, 70°C
14
-
purified recombinant wild-type enzyme, polyphosphate synthesis with GTP
15
-
recombinant truncated enzyme, pH 8.0, 37°C, ATP formation, in presence of 80 mM K+
18
-
purified recombinant wild-type enzyme, polyphosphate synthesis with ITP
20
-
recombinant full-length enzyme, pH 8.0, 37°C, ATP formation, in presence of 80 mM K+
26.7
-
recombinant truncated enzyme, pH 8.0, 37°C, ATP formation, in presence of 25 mM NH4+
31
-
purified recombinant wild-type enzyme, polyphosphate synthesis with ATP
3126.7
-
recombinant full-length enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+ and 80 mM K+
3150
-
recombinant full-length enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+ and 25 mM NH4+
32
-
purified recombinant wild-type enzyme, polyphosphate dephosphorylation
3223.3
-
recombinant full-length enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+
35
-
recombinant full-length enzyme, pH 8.0, 37°C, ATP formation, in presence of 25 mM NH4+
4.2
-
of the recombinant protein
474
synthesis of polyphosphate, pH 7.2, 37°C
645
-
recombinant truncated enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+ and 80 mM K+
655
-
recombinant truncated enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+
715
-
recombinant truncated enzyme, pH 8.0, 37°C, ATP formation, in presence of 5 mM Mg2+ and 25 mM NH4+
99.1
substrate AMP, pH 7, 70°C
additional information