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0.005 - 0.006
(phosphate)45
-
0.0063
(phosphate)46
pH 8.5, temperature not specified in the publication
-
0.0054 - 0.0063
(phosphate)65
-
0.006
(phosphate)66
pH 8.5, temperature not specified in the publication
-
0.011 - 0.29
(phosphate)n+1
29.39
Hexametaphosphate
pH 8.0, 30°C
1.67
phosphate
-
pH 7.0, 30°C
15.2 - 19
polyphosphate(4)
-
0.00311 - 0.00336
polyphosphate25
0.00249 - 0.00311
polyphosphate65
-
3.6
UDP
pH 8.0, 30°C, recombinant isozyme PPK2-3
additional information
additional information
-
0.005
(phosphate)45
pH 8.5, temperature not specified in the publication
-
0.005
(phosphate)45
-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
0.006
(phosphate)45
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.0054
(phosphate)65
pH 8.5, temperature not specified in the publication
-
0.0054
(phosphate)65
-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
0.0063
(phosphate)65
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.011
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
0.013
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
0.29
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
0.03
ADP
-
pH 8.0, 30°C, wild-type enzyme
0.033
ADP
wild-type enzyme, pH and temperature not specified in the publication
0.075
ADP
mutant enzyme N121D, pH and temperature not specified in the publication
0.1
ADP
-
in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C
0.16
ADP
-
isoform PPK1, pH and temperature not specified in the publication
0.176
ADP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
0.18
ADP
-
pH 6.5-8.5, 25°C
0.18
ADP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
0.2
ADP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
0.246
ADP
wild-type, pH not specified in the publication, temperature not specified in the publication
0.358
ADP
mutant N121D, pH not specified in the publication, temperature not specified in the publication
0.38
ADP
pH 8.5, temperature not specified in the publication
0.38
ADP
-
50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
0.546
ADP
wild-type, pH not specified in the publication, temperature not specified in the publication
2
ADP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
2.3
ADP
pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T
2.47
ADP
mutant D62A, pH not specified in the publication, temperature not specified in the publication
2.47
ADP
mutant enzyme D62A, pH and temperature not specified in the publication
2.5
ADP
pH 8.0, 30°C, recombinant isozyme PPK2-2
3.21
ADP
mutant D192A, pH not specified in the publication, temperature not specified in the publication
3.21
ADP
mutant enzyme D192A, pH and temperature not specified in the publication
3.25
ADP
mutant enzyme R178A, pH and temperature not specified in the publication
3.25
ADP
mutant R178A, pH not specified in the publication, temperature not specified in the publication
4.03
ADP
mutant D117N, pH not specified in the publication, temperature not specified in the publication
4.03
ADP
mutant enzyme D117N, pH and temperature not specified in the publication
6.18
ADP
mutant enzyme R118A, pH and temperature not specified in the publication
6.18
ADP
mutant R118A, pH not specified in the publication, temperature not specified in the publication
6.8
ADP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
8
ADP
-
isoform PPK2, pH and temperature not specified in the publication
9.546
ADP
wild-type enzyme, pH and temperature not specified in the publication
12.3
ADP
mutant enzyme K66A, pH and temperature not specified in the publication
12.3
ADP
mutant K66A, pH not specified in the publication, temperature not specified in the publication
0.033
AMP
wild-type, pH not specified in the publication, temperature not specified in the publication
0.075
AMP
mutant N121D, pH not specified in the publication, temperature not specified in the publication
0.246
AMP
wild-type enzyme, pH and temperature not specified in the publication
0.358
AMP
mutant enzyme N121D, pH and temperature not specified in the publication
0.075
ATP
-
pH 7.0, 75°C
0.075
ATP
-
pH 7.0, 75°C, glycogen-complexed enzyme
0.17
ATP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.33
ATP
pH 8.5, temperature not specified in the publication
0.33
ATP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
0.53
ATP
-
ATP in form of MnATP2-
0.8
ATP
-
in 50 mM Tris-HCl pH-7.5, 10 mM NH4SO4,10 mM KPO4, pH 7.0, 4 mM MgCl2, 50 mM NaCl, at 37°C
0.83
ATP
-
pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, detrmined with Robinson and Wood method
1.18
ATP
-
pH 7.0, 20°C, polyphosphate kinase activity in cell extracts, kinetic analysis
1.2
ATP
pH 7.2, room temperature, recombinant polyphosphate kinase
1.35
ATP
-
isoform PPK1, pH and temperature not specified in the publication
1.38
ATP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
1.47
ATP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
1.5 - 2
ATP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
2.06
ATP
pH 7.5, 37°C, mutant enzyme D230N
2.07
ATP
pH 7.5, 37°C, wild-type enzyme
2.08
ATP
pH 7.5, 37°C, mutant enzyme E245K
5.2
ATP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
13
ATP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
0.11
dADP
pH 8.0, 30°C
0.71
dAMP
pH 8.0, 30°C
0.16
GDP
-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
1.2
GDP
pH 8.0, 30°C, recombinant isozyme PPK2-1
11.3
GDP
-
isoform PPK1, pH and temperature not specified in the publication
2.88
GMP
pH 8.0, 30°C
0.14
GTP
-
-
0.14
GTP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.66
GTP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
1
ITP
-
-
1
ITP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.035
Polyphosphate
-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
15
Polyphosphate
-
pH 7.2, 30°C, recombinant wild-type enzyme
15.2
polyphosphate(4)
-
pH 8.0, 30°C, wild-type enzyme
-
19
polyphosphate(4)
pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T
-
0.00311
polyphosphate25
-
pH 8.0, 37°C, full-length enzyme
0.00336
polyphosphate25
-
pH 8.0, 37°C, truncated enzyme
0.00249
polyphosphate65
-
pH 8.0, 37°C, full-length enzyme
-
0.00311
polyphosphate65
-
pH 8.0, 37°C, truncated enzyme
-
additional information
additional information
-
exponential kinetics
-
additional information
additional information
Michalis-Menten steady state kinetic analysis of FtPPK2 substrate specificity, overview
-
additional information
additional information
-
kinetic analysis of recombinant wild-type and truncated mutant enzymes, polyphosphatase activity (EC 3.6.1.11) and polyphosphate:ADP phosphotransferase activity, overview
-