Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) | Pseudomonas aeruginosa |
expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) | Sinorhizobium meliloti |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 7.5), 0.1 M Na-formate, 0.1 M Li-sulfate, and 0.3 M NDSB 211 | Sinorhizobium meliloti |
hanging drop vapor diffusion method, using 0.1 M HEPES-K (pH 7.0), 5% tacsimate, and 10% PEG MME 5K | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D148A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
D223A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
D307A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D320A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D323A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D362A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D437A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
D93A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
E304A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
E90A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
K311A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
K473A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
K97A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
Q202A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
Q416A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R149A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
R205A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
R209A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
R263A | the mutant shows almost wild type activity | Sinorhizobium meliloti |
R316A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R325A | the mutant shows reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R363A | the mutant shows almost no activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R419A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R423A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
R477A | the mutant shows increased activity compared to the wild type enzyme | Pseudomonas aeruginosa |
W194A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
W408A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Y233A | the mutant shows strongly reduced activity compared to the wild type enzyme | Sinorhizobium meliloti |
Y447A | the mutant shows strongly reduced activity compared to the wild type enzyme | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ | Pseudomonas aeruginosa | |
Mg2+ | no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ | Sinorhizobium meliloti |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
gel filtration | Pseudomonas aeruginosa |
124500 | - |
gel filtration | Sinorhizobium meliloti |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Sinorhizobium meliloti | Q92SA6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
- |
Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | - |
Pseudomonas aeruginosa | ADP + (phosphate)n+1 | - |
? | |
ATP + (phosphate)n | - |
Sinorhizobium meliloti | ADP + (phosphate)n+1 | - |
? | |
additional information | PA3455 protein is also active with (phosphate)3 as a phosphodonor, but its activity and substrate affinity are lower than those with (phosphate)12-13 | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Pseudomonas aeruginosa |
homotetramer | x-ray crystallography | Sinorhizobium meliloti |
Synonyms | Comment | Organism |
---|---|---|
family-2 polyphosphate kinase | - |
Pseudomonas aeruginosa |
family-2 polyphosphate kinase | - |
Sinorhizobium meliloti |
PA3455 protein | - |
Pseudomonas aeruginosa |
PPK2 | - |
Pseudomonas aeruginosa |
PPK2 | - |
Sinorhizobium meliloti |
SMc02148 protein | - |
Sinorhizobium meliloti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Pseudomonas aeruginosa |
8 | 9.5 | - |
Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Pseudomonas aeruginosa | |
ATP | - |
Sinorhizobium meliloti |