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2.7.4.1: ATP-polyphosphate phosphotransferase

This is an abbreviated version!
For detailed information about ATP-polyphosphate phosphotransferase, go to the full flat file.

Word Map on EC 2.7.4.1

Reaction

ATP
+
(Phosphate)n
=
ADP
 +
(phosphate)n+1

Synonyms

AAur2811, AjPPK, ATP:polyphosphate phosphotransferase, AUJ86_09545, CHU0107, CHU_0107, class I PPK2, class II polyphosphate kinase, class III PPK2, Deipr_1912, DR0132, DR_0132, ES2, family-2 polyphosphate kinase, FTT_1564, glycogen-bound polyphosphate kinase, GmSuSy, I601_4057, K649_10090, kinase, polyphosphate (phosphorylating), More, MrH2468, Mrub_2488, MXAN_0056, NCg12620, OsIPK2, PA0141, PA3455 protein, paPpx, plyphosphate kinase 1, poly P kinase 1, poly(P) kinase 1, poly(P) kinase 2, poly-P kinase, poly-P kinase 1, polyP kinase, polyphosphate kinase, polyphosphate kinase 1, polyphosphate kinase 2, polyphosphate kinase 3, polyphosphate kinase-1, polyphosphate kinase-2, polyphosphate-dependent nucleoside diphosphate kinase, polyphosphate: ADP phosphotransferase, polyphosphate:ADP phosphotransferase, polyphosphate:nucleotide phosphotransferase, polyphosphoric acid kinase, PPK, PPK-2, PPK1, PPK2, PPK2-1, PPK2-2, PPK2-3, PPK2a, PPK2B, PPK2c, PPK2d, PPK2e, PPK3, Rv3232c, SMc02148, SMc02148 protein, SPO0224, SPO1256, SPO1727

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.1 ATP-polyphosphate phosphotransferase

Crystallization

Crystallization on EC 2.7.4.1 - ATP-polyphosphate phosphotransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor-diffusion method at 18°C, crystal structures of CHU0107 in complex with AMP, ADP, or guanosine 5'-tetraphosphate. The structures of CHU0107-inhibitor complexes reveales at least two binding pockets for aryl phosphonate inhibitors
sitting-drop vapor-diffusion method at 18°C
full-length polyphosphate kinase and its complex with beta-gamma-imidoadenosine 5-phosphate
hanging drop vapor diffusion method at room temperatur, long rod crystals appear after 3 days in a mixture of 0.005 ml protein and 0.005 ml reservoir solution consisting of 12-14% hexandiol, 10 mM dithiothreitol, 100 mM Tris-HCl, pH 7.5, crystals of polyphosphate kinase complexed with AMP-PNP diffract to 2.5 A resolution
-
purified recombinant enzyme, X-ray diffraction structure determination and analysis at 2.23 A resolution, molecular replacement using the Sinorhizobium meliloti PPK2 structure (SMc02148, PDB ID 3CZQ) as a model
structure to 2.23 A resolution, class I PPK2. Comparison with the structure of Meiothermus ruber PKK2, a class III PKK2
structure to 2.23 resolution. The structure consists of a six-stranded parallel beta-sheet surrounded by 12 alpha-helices, with a high degree of similarity to other members of the PPK2 family
vapor diffusion method, enzyme complexed with polyphosphate and/or nucleotide substrate
structure to 1.9 A resolution, as the archetypal class III PPK2. PPK2 forms a crystallographic tetramer, each protomer consists of a five-stranded parallel beta-sheet surrounded by 10 alpha-helices. Comparison with the structure of Francisella tularensis PKK2, a class I PKK2
vapor diffusion method, enzyme complexed with polyphosphate and/or nucleotide substrate
sitting-drop vapor-diffusion method at 18°C
hanging drop vapor diffusion method, using 0.1 M HEPES-K (pH 7.0), 5% tacsimate, and 10% PEG MME 5K
-
hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 7.5), 0.1 M Na-formate, 0.1 M Li-sulfate, and 0.3 M NDSB 211
sitting-drop vapor-diffusion method at 18°C