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0.02 - 50
(phosphate)45
-
53
(phosphate)46
pH 8.5, temperature not specified in the publication
-
0.02 - 53
(phosphate)65
-
50
(phosphate)66
pH 8.5, temperature not specified in the publication
-
5.53 - 132
(phosphate)n+1
43
ITP
-
pH 7.2, 30°C, recombinant wild-type enzyme
5.6 - 15
polyphosphate(4)
-
0.31 - 4.28
polyphosphate25
0.29 - 3.93
polyphosphate65
-
0.02
(phosphate)45
pH 8.5, temperature not specified in the publication
-
0.02
(phosphate)45
-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
50
(phosphate)45
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
0.02
(phosphate)65
pH 8.5, temperature not specified in the publication
-
0.02
(phosphate)65
-
polyphosphate synthesis reaction, in 50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
-
53
(phosphate)65
-
polyphosphate utilization reaction, in 50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
-
5.53
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
29.63
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
132
(phosphate)n+1
pH 8.0, 30°C, cosubstrate: ADP
0.0015
ADP
mutant D192A, pH not specified in the publication, temperature not specified in the publication
0.0022
ADP
mutant R118A, pH not specified in the publication, temperature not specified in the publication
0.0031
ADP
mutant R178A, pH not specified in the publication, temperature not specified in the publication
0.0053
ADP
mutant K66A, pH not specified in the publication, temperature not specified in the publication
0.015
ADP
mutant N121D, pH not specified in the publication, temperature not specified in the publication
0.016
ADP
mutant D62A, pH not specified in the publication, temperature not specified in the publication
0.032
ADP
wild-type, pH not specified in the publication, temperature not specified in the publication
0.072
ADP
mutant D117N, pH not specified in the publication, temperature not specified in the publication
0.15
ADP
mutant enzyme D192A, pH and temperature not specified in the publication
0.22
ADP
mutant enzyme R118A, pH and temperature not specified in the publication
0.246
ADP
wild-type, pH not specified in the publication, temperature not specified in the publication
0.31
ADP
mutant enzyme R178A, pH and temperature not specified in the publication
0.53
ADP
mutant enzyme K66A, pH and temperature not specified in the publication
1.59
ADP
mutant enzyme D62A, pH and temperature not specified in the publication
2.9
ADP
pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T
5.22
ADP
pH 8.0, 30°C, recombinant isozyme PPK2-2
7.24
ADP
mutant enzyme D117N, pH and temperature not specified in the publication
9.1
ADP
-
pH 8.0, 30°C, wild-type enzyme
41.9
ADP
pH 8.5, temperature not specified in the publication
41.9
ADP
-
50 mM Tris-HCl (pH 8.5), 30 mM MgCl2, at 25°C
210
ADP
-
isoform PPK2, pH and temperature not specified in the publication
317
ADP
wild-type enzyme, pH and temperature not specified in the publication
332
ADP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
622
ADP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
943
ADP
-
isoform PPK1, pH and temperature not specified in the publication
950
ADP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
971
ADP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
994
ADP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
2.49
AMP
mutant N121D, pH not specified in the publication, temperature not specified in the publication
7.1
AMP
wild-type, pH not specified in the publication, temperature not specified in the publication
0.05
ATP
pH 8.5, temperature not specified in the publication
0.05
ATP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
44.6
ATP
-
mutant H510Q of isoform PPK1, pH and temperature not specified in the publication
45
ATP
-
mutant H480Q of isoform PPK1, pH and temperature not specified in the publication
45.6
ATP
-
mutant H480A of isoform PPK1, pH and temperature not specified in the publication
46.9
ATP
-
isoform PPK1, pH and temperature not specified in the publication
55
ATP
-
mutant Y524A of isoform PPK1, pH and temperature not specified in the publication
55.4
ATP
-
mutant H510A of isoform PPK1, pH and temperature not specified in the publication
74
ATP
-
pH 7.2, 30°C, recombinant wild-type enzyme
0.55
dADP
pH 8.0, 30°C
0.06
dAMP
pH 8.0, 30°C
0.0883
GDP
-
pH 7.2, 37°C
0.482
GDP
-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
4.18
GDP
pH 8.0, 30°C, recombinant isozyme PPK2-1
645
GDP
-
isoform PPK1, pH and temperature not specified in the publication
0.8
GMP
pH 8.0, 30°C
0.08
GTP
-
50 mM Tris-HCl (pH 8.5), 1 mM MnCl2, at 25°C
34
GTP
-
pH 7.2, 30°C, recombinant wild-type enzyme
1.04
Polyphosphate
-
pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis
77
Polyphosphate
-
pH 7.2, 30°C, recombinant wild-type enzyme
5.6
polyphosphate(4)
-
pH 8.0, 30°C, wild-type enzyme
-
15
polyphosphate(4)
pH 8.0, 30°C, mutant enzyme H102K/A106E/V115T
-
0.31
polyphosphate25
-
pH 8.0, 37°C, truncated enzyme
4.28
polyphosphate25
-
pH 8.0, 37°C, full-length enzyme
0.29
polyphosphate65
-
pH 8.0, 37°C, truncated enzyme
-
3.93
polyphosphate65
-
pH 8.0, 37°C, full-length enzyme
-
0.117
UDP
-
pH 7.2, 37°C
4.9
UDP
pH 8.0, 30°C, recombinant isozyme PPK2-3