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Literature summary for 2.7.4.1 extracted from
- Studies on biosynthesis of polymetaphosphate by an enzyme from Corynebacterium xerosis (1961), Biochim. Biophys. Acta, 54, 121-132.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against 100 mM NaCl or 25 mM Tris-HCl buffer, pH 7.4 at 0-2°C for 15 h completely inactivates, phosphate, boiled cell extract or complex salt mixture does not restore activity | Corynebacterium xerosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | 40 mM, almost complete inhibition | Corynebacterium xerosis |  |
| ADP | - |
Corynebacterium xerosis | |
| AMP | at high concentrations | Corynebacterium xerosis | |
| additional information | not inhibited by NaCl | Corynebacterium xerosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Corynebacterium xerosis | |
| Mg2+ | maximal activity at 12-13 mM | Corynebacterium xerosis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium xerosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, alumina gel, acetone | Corynebacterium xerosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
- |
Corynebacterium xerosis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 6-8 weeks, no loss of activity | Corynebacterium xerosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Corynebacterium xerosis | ADP + (phosphate)n+1 | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Corynebacterium xerosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Corynebacterium xerosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8.8 | approx. half-maximal activity at pH 6.8 and pH 8.8 | Corynebacterium xerosis |
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Release 2023.1 (January 2023)
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