Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | substrate inhibition | Mycolicibacterium smegmatis | |
AMP | 15% and 70% inhibition of the forward reaction at AMP/ADP ratios of 1 and 10, respectiverly | Mycolicibacterium smegmatis | |
ATP | substrate inhibition, at high concentrations | Mycolicibacterium smegmatis | |
EDTA | 10 mM, complete inhibition | Mycolicibacterium smegmatis | |
F- | 5 mM, 75% inhibition | Mycolicibacterium smegmatis | |
Mg2+ | 8 mM, complete inhibition | Mycolicibacterium smegmatis | |
phosphate | 5.5 mM and 11 mM, 33% and 49% inhibition of forward reaction | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Polyphosphate | - |
Mycolicibacterium smegmatis | |
1.1 | - |
ADP | pH 6.0, 37°C | Mycolicibacterium smegmatis | |
2.5 | - |
ATP | pH 6.0, 37°C | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Mycolicibacterium smegmatis | |
Mg2+ | maximal activity at 2 mM | Mycolicibacterium smegmatis | |
Mn2+ | slightly | Mycolicibacterium smegmatis | |
Mn2+ | activation, can replace Mg2+ | Mycolicibacterium smegmatis | |
additional information | not activated by Ca2+ | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, Sephadex G-200 | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.18 | - |
- |
Mycolicibacterium smegmatis |
Storage Stability | Organism |
---|---|
-20°C, 1 month, no loss of activity | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n+1 | substrates are polyphosphates or hexametaphosphate | Mycolicibacterium smegmatis | ATP + (phosphate)n | - |
r | |
ADP + (phosphate)n+1 | no activity with GDP, diphosphate or tripolyphosphate | Mycolicibacterium smegmatis | ATP + (phosphate)n | - |
r | |
ATP + (phosphate)n | - |
Mycolicibacterium smegmatis | ADP + (phosphate)n+1 | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | 6.5 | - |
Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7 | approx. half-maximal activity at pH 5.8, approx. 85% of maximal activity at pH 7.0 | Mycolicibacterium smegmatis |