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Literature summary for 2.7.4.1 extracted from

  • Suzuki, H.; Kaneko, T.; Ikeda, Y.
    Properties of polyphosphate kinase prepared from mycobacterium smegmatis (1972), Biochim. Biophys. Acta, 268, 381-390.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP substrate inhibition Mycolicibacterium smegmatis
AMP 15% and 70% inhibition of the forward reaction at AMP/ADP ratios of 1 and 10, respectiverly Mycolicibacterium smegmatis
ATP substrate inhibition, at high concentrations Mycolicibacterium smegmatis
EDTA 10 mM, complete inhibition Mycolicibacterium smegmatis
F- 5 mM, 75% inhibition Mycolicibacterium smegmatis
Mg2+ 8 mM, complete inhibition Mycolicibacterium smegmatis
phosphate 5.5 mM and 11 mM, 33% and 49% inhibition of forward reaction Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
Polyphosphate
-
Mycolicibacterium smegmatis
1.1
-
ADP pH 6.0, 37°C Mycolicibacterium smegmatis
2.5
-
ATP pH 6.0, 37°C Mycolicibacterium smegmatis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Mycolicibacterium smegmatis
Mg2+ maximal activity at 2 mM Mycolicibacterium smegmatis
Mn2+ slightly Mycolicibacterium smegmatis
Mn2+ activation, can replace Mg2+ Mycolicibacterium smegmatis
additional information not activated by Ca2+ Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-200 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
-
Mycolicibacterium smegmatis

Storage Stability

Storage Stability Organism
-20°C, 1 month, no loss of activity Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (phosphate)n+1 substrates are polyphosphates or hexametaphosphate Mycolicibacterium smegmatis ATP + (phosphate)n
-
r
ADP + (phosphate)n+1 no activity with GDP, diphosphate or tripolyphosphate Mycolicibacterium smegmatis ATP + (phosphate)n
-
r
ATP + (phosphate)n
-
Mycolicibacterium smegmatis ADP + (phosphate)n+1
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 6.5
-
Mycolicibacterium smegmatis

pH Range

pH Minimum pH Maximum Comment Organism
5.8 7 approx. half-maximal activity at pH 5.8, approx. 85% of maximal activity at pH 7.0 Mycolicibacterium smegmatis