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Literature summary for 2.7.4.1 extracted from
- Polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria (2007), Mol. Microbiol., 65, 261-276.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | PPK1 is necessary for survival under anaerobic conditions or oxidative stress | Mycolicibacterium smegmatis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ppk1, expression of wild-type and mutant enzymes | Mycolicibacterium smegmatis |
| gene ppk1, expression of wild-type and mutant enzymes | Mycobacterium tuberculosis |
| overexpression in Escherichia coli | Mycolicibacterium smegmatis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D395A | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| F176A | site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| F176Y | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| G347A | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| H491A | site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| H491A/H510A | site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| H510A | site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| L234A | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| additional information | PPK knockout organism is susceptible to oxidative stress | Mycolicibacterium smegmatis |
| additional information | downregulation of ppk1 leads to impaired survival of Mycobacterium tuberculosis in macrophages, the stringent response regulator ppGpp is impaired in the ppk1 knockout mutant and is restored after complementation with ppk1 of Mycobacterium tuberculosis, overview | Mycobacterium tuberculosis |
| additional information | effect of oxidative and surface stress as well as anaerobiosis on the survival of a ppk1 knockout mutant PPK-KO, overview. Effect of inactivation of ppk1 on the transcription of mprA, mprB, sigE and rel, overview | Mycolicibacterium smegmatis |
| P225A | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| R230A | site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| R230K | site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
| R309A | site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme | Mycolicibacterium smegmatis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mycolicibacterium smegmatis |  |
| Mg2+ | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81000 | - |
Western blot | Mycolicibacterium smegmatis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | Mycolicibacterium smegmatis | - |
ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | Mycobacterium tuberculosis | - |
ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | Mycobacterium tuberculosis H37Rv | - |
ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | Mycolicibacterium smegmatis mc(2)155 | - |
ADP + (phosphate)n+1 | - |
r | |
| additional information | Mycolicibacterium smegmatis | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | ? | - |
? | |
| additional information | Mycobacterium tuberculosis | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview | ? | - |
? | |
| additional information | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | ? | - |
? | |
| additional information | Mycobacterium tuberculosis H37Rv | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview | ? | - |
? | |
| additional information | Mycolicibacterium smegmatis mc(2)155 | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WHV9 | gene ppk | - |
| Mycobacterium tuberculosis H37Rv | P9WHV9 | gene ppk | - |
| Mycolicibacterium smegmatis | - |
- |
- |
| Mycolicibacterium smegmatis | - |
gene ppk1 | - |
| Mycolicibacterium smegmatis mc(2)155 | - |
- |
- |
| Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
gene ppk1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant His x6-tagged protein | Mycolicibacterium smegmatis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative activity of mutant enzymes compared to the wild-type in both reaction directions, overview | Mycolicibacterium smegmatis |
| 0.7 | - |
synthesis of ATP, reversed reaction | Mycolicibacterium smegmatis |
| 4.2 | - |
of the recombinant protein | Mycolicibacterium smegmatis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | - |
Mycolicibacterium smegmatis | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Mycobacterium tuberculosis | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Mycobacterium tuberculosis H37Rv | ADP + (phosphate)n+1 | - |
r | |
| ATP + (phosphate)n | - |
Mycolicibacterium smegmatis mc(2)155 | ADP + (phosphate)n+1 | - |
r | |
| additional information | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | Mycolicibacterium smegmatis | ? | - |
? | |
| additional information | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
| additional information | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium tuberculosis, overview | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| additional information | polyphosphate kinase is involved in stress-induced mprAB-sigE-rel signalling in mycobacteria, mprAB-sigE-rel signalling cascade, overview. PPK1 is required for the stringent response, under conditions of stress polyP acts as a preferred donor for MprB-mediated phosphorylation of MprA facilitating transcription of the sigE gene thereby leading finally to the enhancement of the transcription of rel in Mycobacterium smegmatis, overview | Mycolicibacterium smegmatis mc(2)155 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Mycolicibacterium smegmatis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP:polyphosphate phosphotransferase | - |
Mycolicibacterium smegmatis |
| kinase, polyphosphate (phosphorylating) | - |
Mycolicibacterium smegmatis |
| polyphosphate kinase | - |
Mycolicibacterium smegmatis |
| polyphosphate kinase 1 | - |
Mycolicibacterium smegmatis |
| polyphosphate kinase 1 | - |
Mycobacterium tuberculosis |
| PPK1 | - |
Mycolicibacterium smegmatis |
| PPK1 | - |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycolicibacterium smegmatis | |
| ATP | - |
Mycobacterium tuberculosis | |
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