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2.7.4.1: ATP-polyphosphate phosphotransferase

This is an abbreviated version!
For detailed information about ATP-polyphosphate phosphotransferase, go to the full flat file.

Word Map on EC 2.7.4.1

Reaction

ATP
+
(Phosphate)n
=
ADP
+
(phosphate)n+1

Synonyms

AAur2811, AjPPK, ATP:polyphosphate phosphotransferase, AUJ86_09545, CHU0107, CHU_0107, class I PPK2, class II polyphosphate kinase, class III PPK2, Deipr_1912, DR0132, DR_0132, ES2, family-2 polyphosphate kinase, FTT_1564, glycogen-bound polyphosphate kinase, GmSuSy, I601_4057, K649_10090, kinase, polyphosphate (phosphorylating), More, MrH2468, Mrub_2488, MXAN_0056, NCg12620, OsIPK2, PA0141, PA3455 protein, paPpx, plyphosphate kinase 1, poly P kinase 1, poly(P) kinase 1, poly(P) kinase 2, poly-P kinase, poly-P kinase 1, polyP kinase, polyphosphate kinase, polyphosphate kinase 1, polyphosphate kinase 2, polyphosphate kinase 3, polyphosphate kinase-1, polyphosphate kinase-2, polyphosphate-dependent nucleoside diphosphate kinase, polyphosphate: ADP phosphotransferase, polyphosphate:ADP phosphotransferase, polyphosphate:nucleotide phosphotransferase, polyphosphoric acid kinase, PPK, PPK-2, PPK1, PPK2, PPK2-1, PPK2-2, PPK2-3, PPK2a, PPK2B, PPK2c, PPK2d, PPK2e, PPK3, Rv3232c, SMc02148, SMc02148 protein, SPO0224, SPO1256, SPO1727

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.4 Phosphotransferases with a phosphate group as acceptor
                2.7.4.1 ATP-polyphosphate phosphotransferase

Engineering

Engineering on EC 2.7.4.1 - ATP-polyphosphate phosphotransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D230N
the variant has somewhat greater processivity than the wild-type enzyme
E173K
the variant has somewhat greater processivity than the wild-type enzyme
E245K
mutation leads to very high polyP accumulation in vivo but is not different from the wild type in either activity or chain length of polyP produced in vitro
F488A
-
complete loss of all 4 polyphosphate kinase activities
P507A
-
complete loss of all 4 polyphosphate kinase activities
Q674A
-
complete loss of all 4 polyphosphate kinase activities
R375A
-
complete loss of all 4 polyphosphate kinase activities
R564A
-
complete loss of all 4 polyphosphate kinase activities
R621A
-
complete loss of all 4 polyphosphate kinase activities
S380A
-
complete loss of all 4 polyphosphate kinase activities
Y468A
-
120-140% of wild-type polyphosphate, GTP, and guanosine 5'-tetraphosphate synthesis activity, 20-50% of wild-type ATP synthesis activity
D230N
-
the variant has somewhat greater processivity than the wild-type enzyme
-
E173K
-
the variant has somewhat greater processivity than the wild-type enzyme
-
E245K
-
mutation leads to very high polyP accumulation in vivo but is not different from the wild type in either activity or chain length of polyP produced in vitro
-
D117N
D192A
R118A
R178A
D192A
D62A
-
about 1000fold decrease in activity
-
E126G
about 18% of wild-type activity with substrate AMP, about 40% of wild-type activity with ADP
E126N
about 5% of wild-type activity with substrate AMP, bout 15% of wild-type activity with ADP
N121D
E126G
-
about 18% of wild-type activity with substrate AMP, about 40% of wild-type activity with ADP
-
E126N
-
about 5% of wild-type activity with substrate AMP, bout 15% of wild-type activity with ADP
-
N121D
-
7fold decrease in activity with AMP, 25fold decrease in activity with ADP
-
A615S
-
the mutant exhibits about wild type activity for ATP synthesis and polyphosphate synthesis
E681A
-
the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared o the wild type enzyme
F125A
the proteins has significantly reduced activity as compared to wild type protein
F67A
-
84% activity compared to the wild type enzyme
G69R
-
67.1% activity compared to the wild type enzyme
G72A
the activity of MBP-G72APPK-2 and wild type proteins are similar
G74R
-
7.6% activity compared to the wild type enzyme, the mutant enzyme cannot interact with nucleoside diphosphate kinase
H115A
-
49% activity compared to the wild type enzyme
H115A/H247A
-
6% activity compared to the wild type enzyme
H150A
-
the mutation causes PPK1 protein deficient but not defective in autophosphorylation and severely affects ATP or polyphosphate synthesis
H150Q
-
the mutation causes PPK1 protein deficient but not defective in autophosphorylation and mildly affects ATP or polyphosphate synthesis
H247A
-
38% activity compared to the wild type enzyme
H480A
-
the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme
H480Q
-
the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme
H510Q
-
the mutant catalytic efficiency for the polyphosphate synthesis is almost or very close to that of the wild type enzyme
K75A
-
89% activity compared to the wild type enzyme
N515A
-
the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme
R431A
-
the mutant exhibits less than 5% activity for ATP synthesis and about 78% activity for polyphosphate synthesis compared to the wild type enzyme
R461A
-
the mutant exhibits less than 2% activity for ATP synthesis and about 76% activity for polyphosphate synthesis compared to the wild type enzyme
R624A
-
the mutant exhibits less than 2% activity for ATP synthesis and about 65% activity for polyphosphate synthesis compared to the wild type enzyme
R654A
-
the mutant exhibits less than 1% activity for ATP synthesis and about 90% activity for polyphosphate synthesis compared to the wild type enzyme
S668A
-
the mutant exhibits less than 5% activity for ATP synthesis and less than 30% activity for polyphosphate synthesis compared to the wild type enzyme
W129A
the proteins has significantly reduced activity as compared to wild type protein
Y524A
-
the mutant exhibits less than 10% activity for ATP synthesis and less than 20% activity for polyphosphate synthesis compared to the wild type enzyme
F125A
-
the proteins has significantly reduced activity as compared to wild type protein
-
G72A
-
the activity of MBP-G72APPK-2 and wild type proteins are similar
-
W129A
-
the proteins has significantly reduced activity as compared to wild type protein
-
F125A
-
the proteins has significantly reduced activity as compared to wild type protein
-
F67A
-
84% activity compared to the wild type enzyme
-
G69R
-
67.1% activity compared to the wild type enzyme
-
G72A
-
the activity of MBP-G72APPK-2 and wild type proteins are similar
-
H115A
-
49% activity compared to the wild type enzyme
-
H247A
-
38% activity compared to the wild type enzyme
-
K75A
-
89% activity compared to the wild type enzyme
-
W129A
-
the proteins has significantly reduced activity as compared to wild type protein
-
D395A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
F176A
-
site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme
F176Y
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
G347A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
H491A
-
site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme
H491A/H510A
-
site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme
H510A
-
site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme
L234A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
P225A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
R230A
-
site-directed mutagenesis, highly reduced forward and reverse reaction activities compared to the wild-type enzyme
R230K
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
R309A
-
site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme
G347A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
-
H510A
-
site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme
-
R230K
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
-
G347A
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
-
H510A
-
site-directed mutagenesis, reduced forward and reverse reaction activities compared to the wild-type enzyme
-
R230K
-
site-directed mutagenesis, slightly reduced forward and reverse reaction activities compared to the wild-type enzyme
-
D307A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
D320A
-
the mutant shows reduced activity compared to the wild type enzyme
D323A
-
the mutant shows reduced activity compared to the wild type enzyme
D362A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
D437A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
E304A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
K311A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
K473A
-
the mutant shows reduced activity compared to the wild type enzyme
Q416A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
R316A
-
the mutant shows reduced activity compared to the wild type enzyme
R325A
-
the mutant shows reduced activity compared to the wild type enzyme
R363A
-
the mutant shows almost no activity compared to the wild type enzyme
R419A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
R423A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
R477A
-
the mutant shows increased activity compared to the wild type enzyme
W408A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
Y447A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
A106E
mutant enzyme does not show any detectable activity
A106E/H102K
mutant enzyme does not show any detectable activity
A106E/V115T
mutant enzyme does not show any detectable activity
D148A
the mutant shows strongly reduced activity compared to the wild type enzyme
D223A
the mutant shows strongly reduced activity compared to the wild type enzyme
D93A
the mutant shows strongly reduced activity compared to the wild type enzyme
E90A
the mutant shows strongly reduced activity compared to the wild type enzyme
H102K
mutant enzyme does not show any detectable activity
H102K/A106E/V115T
mutant enzyme H102K/A106E/V115T exhibits the capability to utilize polyP(4) as phosphate donor to synthesize ATP. The wild-type enzyme can not adopt polyphosphate(4)
K97A
the mutant shows strongly reduced activity compared to the wild type enzyme
Q202A
the mutant shows strongly reduced activity compared to the wild type enzyme
R149A
the mutant shows strongly reduced activity compared to the wild type enzyme
R205A
the mutant shows strongly reduced activity compared to the wild type enzyme
R209A
the mutant shows strongly reduced activity compared to the wild type enzyme
R263A
the mutant shows almost wild type activity
V115T
mutant enzyme does not show any detectable activity
V115T/H102K
mutant enzyme does not show any detectable activity
W194A
the mutant shows strongly reduced activity compared to the wild type enzyme
Y233A
the mutant shows strongly reduced activity compared to the wild type enzyme
additional information