Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | 10fold higher rate of polyphosphate synthesis | Propionibacterium freudenreichii subsp. shermanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
native PAGE | Propionibacterium freudenreichii subsp. shermanii |
85000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DE52, hydroxylapatite | Propionibacterium freudenreichii subsp. shermanii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (phosphate)n = ADP + (phosphate)n+1 | short chain polyphosphates are incorporated at the ends of the long chain product | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.73 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Storage Stability | Organism |
---|---|
4°C, in 20 mM Tris-HCl buffer, pH 7.6, 200 mM NaCl, 10% glycerol, 1 mM EDTA, 0.7 mM 2-mercaptoethanol, at least 1 month | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (phosphate)n | strictly processive mechanism of polyphosphate elongation, phosphate or short-chains of polyphosphates serve as primers, majority of the synthesized polyphosphates is 750 residues long | Propionibacterium freudenreichii subsp. shermanii | ADP + (phosphate)n+1 | polyphosphate as primer: product with 750 residues, phosphate as primer: product with 300-2000 residues, i.e. major form with chain length of 2000 | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |