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Literature summary for 2.7.4.1 extracted from
- metaphosphate synthesis by an enzyme from Escherichia coli (1956), Biochim. Biophys. Acta, 20, 215-227.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | - |
Escherichia coli |  |
General Stability
| General Stability | Organism |
|---|---|
| stable to repeated freeze-thawing cycles | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 0.08 mM, complete inhibition | Escherichia coli | |
| diphosphate | weak inhibition | Escherichia coli | |
| F- | 5 mM, complete inhibition | Escherichia coli | |
| additional information | not inhibited by 2,4-dinitrophenol or potassium arsenate | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| streptomycin sulfate, ammonium sulfate | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.07 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -13°C, at least 6 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (phosphate)n | incorporates gamma-phosphate of ATP into long-chain polyphosphate molecules | Escherichia coli | ADP + (phosphate)n+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, 27% loss of activity | Escherichia coli |
| 60 | - |
5 min, 93% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
phosphate buffer less effective than Tris-HCl buffer | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.2 | approx. 45% of maximal activity at pH 6.2, approx. 75% of maximal activity at pH 8.2 | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
10 min, acetate buffer treatment, 90% loss of activity at 0°C | Escherichia coli |
| 5 | - |
10 min, acetate buffer treatment, 70% loss of activity at 0°C | Escherichia coli |
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