Cloned (Comment) | Organism |
---|---|
gene SPO0224, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-3 in Escherichia coli strain BL21(DE3)-T1 | Ruegeria pomeroyi |
gene SPO1256, DNA and amino acid sequence determination and analysis, recombinant expression of STrep-tagged isoyzme PPK2-2 in Escherichia coli strain BL21(DE3)-T1 | Ruegeria pomeroyi |
gene SPO1727, DNA and amino acid sequence determination and analysis, recombinant expression of Strep-tagged isozyme PPK2-1 in Escherichia coli strain BL21(DE3)-T1 | Ruegeria pomeroyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | complete inhibition; complete inhibition; complete inhibition | Ruegeria pomeroyi | |
EDTA | low inhibition; low inhibition | Ruegeria pomeroyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
GDP | pH 8.0, 30°C, recombinant isozyme PPK2-1 | Ruegeria pomeroyi | |
2.5 | - |
ADP | pH 8.0, 30°C, recombinant isozyme PPK2-2 | Ruegeria pomeroyi | |
3.6 | - |
UDP | pH 8.0, 30°C, recombinant isozyme PPK2-3 | Ruegeria pomeroyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Ruegeria pomeroyi | |
Ca2+ | activates, best cation | Ruegeria pomeroyi | |
Mg2+ | activates | Ruegeria pomeroyi | |
Mg2+ | activates, best cation | Ruegeria pomeroyi | |
Mn2+ | activates | Ruegeria pomeroyi | |
Mn2+ | activates, best cation | Ruegeria pomeroyi | |
additional information | enzyme SPO1727 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview | Ruegeria pomeroyi | |
additional information | isozyme SPO0224 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview | Ruegeria pomeroyi | |
additional information | isozyme SPO125 requires a divalent cation, but shows no specificity, only Co2+ is not accepted, overview | Ruegeria pomeroyi | |
Ni2+ | activates | Ruegeria pomeroyi | |
Zn2+ | activates | Ruegeria pomeroyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34750 | - |
calculated from sequence, SDS-PAGE | Ruegeria pomeroyi |
36720 | - |
calculated from sequence, SDS-PAGE | Ruegeria pomeroyi |
39950 | - |
calculated from sequence, SDS-PAGE | Ruegeria pomeroyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n | Ruegeria pomeroyi | - |
ATP + (phosphate)n-1 | - |
? | |
ADP + (phosphate)n | Ruegeria pomeroyi ATCC 700808 | - |
ATP + (phosphate)n-1 | - |
? | |
CDP + (phosphate)n | Ruegeria pomeroyi | - |
CTP + (phosphate)n-1 | - |
? | |
CDP + (phosphate)n | Ruegeria pomeroyi ATCC 700808 | - |
CTP + (phosphate)n-1 | - |
? | |
GDP + (phosphate)n | Ruegeria pomeroyi | - |
GTP + (phosphate)n-1 | - |
? | |
GDP + (phosphate)n | Ruegeria pomeroyi ATCC 700808 | - |
GTP + (phosphate)n-1 | - |
? | |
UDP + (phosphate)n | Ruegeria pomeroyi | - |
UTP + (phosphate)n-1 | - |
? | |
UDP + (phosphate)n | Ruegeria pomeroyi ATCC 700808 | - |
UTP + (phosphate)n-1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruegeria pomeroyi | Q5LSN8 | gene SPO1727, isozyme PPK2-1 | - |
Ruegeria pomeroyi | Q5LU04 | gene SPO1256, isozyme PPK2-2 | - |
Ruegeria pomeroyi | Q5LX16 | gene SPO0224, isozyme PPK2-3 | - |
Ruegeria pomeroyi ATCC 700808 | Q5LSN8 | gene SPO1727, isozyme PPK2-1 | - |
Ruegeria pomeroyi ATCC 700808 | Q5LU04 | gene SPO1256, isozyme PPK2-2 | - |
Ruegeria pomeroyi ATCC 700808 | Q5LX16 | gene SPO0224, isozyme PPK2-3 | - |
Purification (Comment) | Organism |
---|---|
recombinant Strep-tagged isozyme PPK2-1 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography | Ruegeria pomeroyi |
recombinant Strep-tagged isozyme PPK2-2 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography | Ruegeria pomeroyi |
recombinant Strep-tagged isozyme PPK2-3 from Escherichia coli strain BL21(DE3) by streptavidin affinity chromatography | Ruegeria pomeroyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (phosphate)n | - |
Ruegeria pomeroyi | ATP + (phosphate)n-1 | - |
? | |
ADP + (phosphate)n | - |
Ruegeria pomeroyi ATCC 700808 | ATP + (phosphate)n-1 | - |
? | |
CDP + (phosphate)n | - |
Ruegeria pomeroyi | CTP + (phosphate)n-1 | - |
? | |
CDP + (phosphate)n | - |
Ruegeria pomeroyi ATCC 700808 | CTP + (phosphate)n-1 | - |
? | |
GDP + (phosphate)n | - |
Ruegeria pomeroyi | GTP + (phosphate)n-1 | - |
? | |
GDP + (phosphate)n | - |
Ruegeria pomeroyi ATCC 700808 | GTP + (phosphate)n-1 | - |
? | |
additional information | all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity | Ruegeria pomeroyi | ? | - |
? | |
additional information | all PPK isozymes from Ruegeria pomeroyi accept all NDPs similarly and exhibit low NDP selectivity | Ruegeria pomeroyi ATCC 700808 | ? | - |
? | |
UDP + (phosphate)n | - |
Ruegeria pomeroyi | UTP + (phosphate)n-1 | - |
? | |
UDP + (phosphate)n | - |
Ruegeria pomeroyi ATCC 700808 | UTP + (phosphate)n-1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34750, recombinant PPK2-3, sequence calculation and SDS-PAGE | Ruegeria pomeroyi |
monomer | 1 * 36720, recombinant PPK2-1, sequence calculation and SDS-PAGE | Ruegeria pomeroyi |
monomer | 1 * 39950, recombinant PPK2-2, sequence calculation and SDS-PAGE | Ruegeria pomeroyi |
Synonyms | Comment | Organism |
---|---|---|
polyphosphate kinase 2 | - |
Ruegeria pomeroyi |
PPK2 | - |
Ruegeria pomeroyi |
PPK2-1 | - |
Ruegeria pomeroyi |
PPK2-2 | - |
Ruegeria pomeroyi |
PPK2-3 | - |
Ruegeria pomeroyi |
SPO0224 | - |
Ruegeria pomeroyi |
SPO1256 | - |
Ruegeria pomeroyi |
SPO1727 | - |
Ruegeria pomeroyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ruegeria pomeroyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.18 | - |
GDP | pH 8.0, 30°C, recombinant isozyme PPK2-1 | Ruegeria pomeroyi | |
4.9 | - |
UDP | pH 8.0, 30°C, recombinant isozyme PPK2-3 | Ruegeria pomeroyi | |
5.22 | - |
ADP | pH 8.0, 30°C, recombinant isozyme PPK2-2 | Ruegeria pomeroyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Ruegeria pomeroyi |
8 | - |
optimum 1 | Ruegeria pomeroyi |
9 | - |
- |
Ruegeria pomeroyi |
10 | - |
optimum 2 | Ruegeria pomeroyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | activity range, profile overview | Ruegeria pomeroyi |
General Information | Comment | Organism |
---|---|---|
evolution | in general, all PPKs are ancient enzymes, and phylogenetic analysis indicates that the polyphosphate degradation process is older than polyphosphate synthesis. Additionally, PPK2 proteins are older than PPK1 enzymes | Ruegeria pomeroyi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.37 | - |
UDP | pH 8.0, 30°C, recombinant isozyme PPK2-3 | Ruegeria pomeroyi | |
2.08 | - |
ADP | pH 8.0, 30°C, recombinant isozyme PPK2-2 | Ruegeria pomeroyi | |
3.48 | - |
GDP | pH 8.0, 30°C, recombinant isozyme PPK2-1 | Ruegeria pomeroyi |