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3.6.1.1: inorganic diphosphatase

This is an abbreviated version!
For detailed information about inorganic diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.1

Reaction

diphosphate
+
H2O
= 2 phosphate

Synonyms

acid PPase, AT727_13205, AtPPA1, AtPPsPase1, BT2127, CBS-PPase, CBS-PPase1, chloroplast inorganic pyrophosphatase 1, class A type PPA, cobalt-dependent inorganic pyrophosphatase, CPE2055, cytosolic PPase, D1C enzyme, dhPPase, E-PPase, EcPpA, EcPPiase, ehPPase, family I inorganic pyrophosphatase, family I PPase, family I pyrophosphatase, family II inorganic pyrophosphatase, family II PPase, H+-PPase, H+-pyrophosphatase, H+-translocating/vacuolar inorganic pyrophosphatase, Hpp1 protein, HVO_0729, HvPPA, hyperthermophilic inorganic pyrophosphatase, inorganic diphosphatase, inorganic PPase, inorganic pyrophosphatase, inorganic pyrophosphatase 1, inorganic pyrophosphatase-like protein, inorganic pyrophosphorylase, IPP, IPP1, Ipp1p, IPPase, LHPPase, M-PPase, manganese-dependent inorganic pyrophosphatase, MazG, MdPPa, membrane-integral inorganic pyrophosphatase, membrane-integral pyrophosphatase, miPPase, mitochondrial pyrophosphatase, Mn2+-bound canonical Family II PPase, More, Mtb PPiase, Mthe_0236, MtPPA1, MtPPiase, nucleotide-regulated pyrophosphatase, OVP1, p26.1a, p26.1b, PfPPase, PH1907, Pho-PPase, phospholysine phosphohistidine inorganic pyrophosphate phosphatase, phosphoprotein p26.1, PPA, PPA1, PpaC, PPase, PPase 1, PPase 2, ppase-1, PPase-2, PPase1, PPase2, PPase4, PPias, PPIase, proton-translocating inorganic pyrophosphatase, PYP-1, pyrophosphatase, pyrophosphatase, inorganic, Pyrophosphate phospho-hydrolase, pyrophosphate phospho-hydrolase 1, Pyrophosphate phosphohydrolase, Pyrophosphate-energized inorganic pyrophosphatase, RmPPase, Rv3628, siPPiase, soluble inorganic pyrophosphatase, soluble inorganic pyrophosphatase 2, soluble pyrophosphatase, soluble-type yeast PPase, sPPAse, ST2226, Tc-sPPase, TC004566, TcasGA2_TC004566, TgPPase, ThPP1, Tm-PPase, TM0913, V-PPase, vacuolar H(+)-PPase, vacuolar H(+)-pyrophosphatase, vacuolar H+-pyrophosphatase, vacuolar H+-translocating inorganic pyrophosphatase, vacuolar H+-translocating pyrophosphatase, vacuolar pyrophosphatase, VHP, VP1, VSP, VVPP1

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.1 inorganic diphosphatase

Cloned

Cloned on EC 3.6.1.1 - inorganic diphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
At1g73010, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain Bl21 (DE3)
DNA and amino acid sequence determination and analysis, expression in Escherichia coli
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 pLysS(DE3)
DNA and amino acid sequence determination and analysis, genetic structure, functional expression of the His-tagged liver enzyme in Escherichia coli, transcription start site determination by primer extension analysis, protein-DNA interaction analysis of putative transcription factors and binding sites, overview
DQ978330
expressed as a chimaeric protein (fused with secreted invertase precursor SUC2) in Saccharomyces cerevisiae mutant strain YPC3
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells as a fusion protein with a C-terminal polyhistidine tag
-
expressed in Escherichia coli BL21(DE3) cells as a His-tagged protein
-
expressed in Escherichia coli MV1184
expressed in Escherichia coli Rosetta 2 cells
expressed in Escherichia coli Rosetta(DE3)pLysS cells
-
expressed in Escherichia coli XL2
-
expressed in Escherichia LMG194 cells
expressed in Escherichia Rosetta(DE3)pLysS cells
expresses in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli strain BL21
-
expression in Escherichia coli. Co-expression of tRNA-ARG, cognate for the rare codon AGA in Escherichia coli, improves yield
-
expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3)
expression of GST-tagged isozymes in Escherichia coli
expression of His-tagged enzyme
-
expression of His-tagged enzyme in Escherichia coli
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of mutant enzymes: P103A, P122A, S123A, H125A, S158A, R159A, G163A, H165A, P167A, and D169A in Escherichia coli. The expression level varies from 49 to 165% of that of the wild type enzyme
expression of the enzyme in Escherichia coli BL21(DE3)
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
family I and family II isozymes, phylogenetic analysis, family II PPase is laterally transferred to the Vibrionales ancestor and partially degenerated due to functional redundancy, but nevertheless remains fixed as an adjunct to the family I enzyme, overexpression of PPase1 and PPase2 in Escherichia coli
-
gene AT727_13205, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21
gene Hp0620, structure-based phylogenetic tree of diphosphatases, expression of His-tagged enzyme in Escherichia coli strain B834(DE3) resulting in a selenomethionine-enzyme
-
gene ipp, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Haloferax volcanii strain H26-pJAM2920
gene IPP1, recombinant expression in Arabidopsis thaliana fugu5 mutant guard cells
gene OVP1, transformation into rice calli, driven by the maize ubiquitin promoter, with both GUS gene and hygromycin-resistance gene as selection markers by an Agrobacterium-mediated transformation approach
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity
gene ppa, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity, expression in Escherichia coli strain XL1-blue
gene ppa, DNA and amino acid sequence determination and analysis,phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity
gene ppa, expression in rosette source leaf cytosol of Arabidopsis thaliana
-
gene ppa, phylogenetic analysis, functional complmentation of a slow-growing Escherichia coli JP5 mutant strain containing a very low level of soluble inorganic pyrophosphatase activity
-
gene ppa, recombinant expression of the His-tagged enzyme in Escherichia coli strain Rosetta-Gami B (DE3)pLysS
gene ppa1, isozyme PPase 1, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli
gene PPA1, isozyme PPase1, DNA and amino acid sequence determination and anaylsis
gene ppa1, isozyme sPPase-I, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene PPA2, isozyme PPase2, DNA and amino acid sequence determination and anaylsis, localization on chromosome 4q25, expression of a GFP-atgged PPase2 fragment in HeLa cells
gene ppa2, isozyme sPPase-II, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene pyp-1, DNA and amino acid sequence determination and analysis, genomic organization, expression in transformed adult progeny worms using the promoterless GFP vector, pPD95.75 vector
gene TcasGA2_TC004566, real-time PCR expression analysis
gene ThPP1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Oryza sativa subsp. japonica cv. Kitaake shoot and root using the transfection method via Agrobacterium tumefaciens strain GV3101, stable integration of the ThPP1 gene in the transgenic rice genome, construction of a ThPP1::16318hGFP protein expression vector and expression in in onion epidermal cells, quantitative RT-PCR expression analysis
gene VPP, DNA from leaves, DNA and amino acid sequence analysis and transcriptional profiling
gene vpp2, DNA from leaves, DNA and amino acid sequence analysis and transcriptional profiling
gene VVPP1, DNA from leaves, DNA and amino acid sequence analysis and transcriptional profiling
genes MdVHP1 and MdVHP2, phylogenetic analysis, overexpression of MdVHP1 in Solanum lycopersicum cv. Tianjinbaiguo and in Malus domesticus callus tissue, both using the Agrobacterium tumefaciens strain LBA4404 transfection method
heterologous expression in Saccharomyces cerevisiae
MdPPa is transiently expressed in Malus domestica cv. Ralls Janet pollen as GFP fusion protein driven by the tomato pollen-specific promoter Lat52, the MdPPa-GFP constructis transformed into pollen by particle bombardment. Recombinant transient expression of coding sequences (CDSs) of MdPPa and S1-, S2-, S3- and S9-RNase from pEZS-NL vector driven by CaMV 35S promoter in Zea mays protoplasts. Recombinant coexpression of MBP-tagged S1, S2, S3, S9-RNase and His6-tagged A14 in Escherichia coli
XM_008360526
overexpression of soluble wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
quantitative RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21
-
quantitative RT-PCR expression analysis, recombinant expression of MBP-His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21
quantitative tissue-specific expression analysis
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli
recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21(DE3)
subcloning in Escherichia coli strain XL1-blue, expression of wild-type and mutant enzymes in enzyme-deficient Saccharomyces cerevisiae strain BJ268
-