Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Aquifex aeolicus |
Application | Comment | Organism |
---|---|---|
molecular biology | cycle sequencing methods | Aquifex aeolicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MV1184 | Aquifex aeolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Aquifex aeolicus | |
Cu2+ | - |
Aquifex aeolicus | |
EDTA | - |
Aquifex aeolicus | |
Hg2+ | - |
Aquifex aeolicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | minor activation | Aquifex aeolicus | |
Mg2+ | required | Aquifex aeolicus | |
Mn2+ | minor activation | Aquifex aeolicus | |
Zn2+ | about the same activation as with Mg2+ | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
gel filtration | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | Aquifex aeolicus | important for energy metabolism, provides energy for biosynthetic reactions | 2 phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67501 | hyperthermophilic bacterium | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.13 | - |
purified enzyme, pH 8.0, 80°C | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Aquifex aeolicus | 2 phosphate | - |
? | |
diphosphate + H2O | important for energy metabolism, provides energy for biosynthetic reactions | Aquifex aeolicus | 2 phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 85 | - |
Aquifex aeolicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | - |
Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
50% of activity lost within 90 min | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Aquifex aeolicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Aquifex aeolicus |