Cloned (Comment) | Organism |
---|---|
expressed in Escherichia LMG194 cells | Mycoplasma suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzymatic activity is inhibited more than 95% in the presence of 5 mM Ca2+ | Mycoplasma suis | |
EDTA | enzymatic activity is inhibited more than 70% in the presence of 5 mM EDTA | Mycoplasma suis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Mycoplasma suis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the activity of the enzyme is strongly dependent on Mg2+. High enzyme activity is found between 1 and 100 mM Mg2+ with a maximum activity at 10 mM. At pH 7.5, the maximum PPase activity is at 50 mM Mg2+ | Mycoplasma suis | |
Mn2+ | substitution of Mg2+ cations with Mn2+ results in significantly lower activity of 25.34% of the Mg2+-induced activity | Mycoplasma suis | |
Zn2+ | substitution of Mg2+ cations with Zn2+ results in significantly lower activity of 14.3% of the Mg2+-induced activity | Mycoplasma suis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18600 | - |
4 * 18600, calculated from amino acid sequence | Mycoplasma suis |
20000 | - |
4 * 20000, SDS-PAGE | Mycoplasma suis |
80000 | - |
gel filtration | Mycoplasma suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma suis | C4RA40 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Mycoplasma suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | - |
Mycoplasma suis | phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 20000, SDS-PAGE | Mycoplasma suis |
homotetramer | 4 * 18600, calculated from amino acid sequence | Mycoplasma suis |
Synonyms | Comment | Organism |
---|---|---|
inorganic pyrophosphatase | - |
Mycoplasma suis |
PPase | - |
Mycoplasma suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Mycoplasma suis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | at conditions below pH 7.5 and above pH 10.0 activity decreases considerably | Mycoplasma suis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycoplasma suis | calculated from amino acid sequence | - |
4.7 |